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Bacillus Krulwichiae
''Alkalihalobacillus krulwichiae'' is a Gram-positive, obligate alkaliphilic and facultatively anaerobic bacterium from the genus ''Alkalihalobacillus'' which has been isolated from soil from Tsukuba is a city located in Ibaraki Prefecture, Japan. As of January 1, 2024, the city had an estimated population of 256,526 in 121,001 households and a population density of 900 persons per km2. The percentage of the population aged over 65 wa .... References Bacillaceae Bacteria described in 2003 {{Bacilli-stub ...
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Gram-positive
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is used by microbiologists to place bacteria into two main categories, gram-positive (+) and gram-negative bacteria, gram-negative (−). Gram-positive bacteria have a thick layer of peptidoglycan within the cell wall, and gram-negative bacteria have a thin layer of peptidoglycan. Gram-positive bacteria retain the crystal violet stain used in the test, resulting in a purple color when observed through an optical microscope. The thick layer of peptidoglycan in the bacterial cell wall retains the Stain (biology), stain after it has been fixed in place by iodine. During the decolorization step, the decolorizer removes crystal violet from all other cells. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization ...
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Alkaliphilic
Alkaliphiles are a class of extremophilic microbes capable of survival in alkaline ( pH roughly 8.5–11) environments, growing optimally around a pH of 10. These bacteria can be further categorized as obligate alkaliphiles (those that require high pH to survive), facultative alkaliphiles (those able to survive in high pH, but also grow under normal conditions) and haloalkaliphiles (those that require high salt content to survive).Horikoshi, Koki. "Alkaliphiles: Some applications of their products for biotechnology." Microbiology and Molecular Biology Reviews 63.4 (1999): 735-50. Print. Background information Microbial growth in alkaline conditions presents several complications to normal biochemical activity and reproduction, as high pH is detrimental to normal cellular processes. For example, alkalinity can lead to denaturation of DNA, instability of the plasma membrane and inactivation of cytosolic enzymes, as well as other unfavorable physiological changes.Higashibata, Akira, ...
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Facultatively Anaerobic
A facultative anaerobic organism is an organism that makes ATP by aerobic respiration if oxygen is present, but is capable of switching to fermentation if oxygen is absent. Some examples of facultatively anaerobic bacteria are ''Staphylococcus'' spp., ''Escherichia coli'', ''Salmonella'', ''Listeria'' spp., '' Shewanella oneidensis'' and ''Yersinia pestis''. Certain eukaryotes are also facultative anaerobes, including pupfish, fungi such as ''Saccharomyces cerevisiae'' and many aquatic invertebrates such as nereid polychaetes. It has been observed that in mutants of ''Salmonella typhimurium'' that underwent mutations to be either obligate aerobes or anaerobes, there were varying levels of chromatin-remodeling proteins. The obligate aerobes were later found to have a defective DNA gyrase subunit A gene ('' gyrA''), while obligate anaerobes were defective in topoisomerase I (''topI''). This indicates that topoisomerase I and its associated relaxation of chromosomal DNA is requir ...
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Alkalihalobacillus
''Alkalihalobacillus'' is a genus of gram-positive or gram-variable rod-shaped bacteria in the family '' Bacillaceae'' from the order ''Bacillales''. The type species of this genus is '' Alkalihalobacillus alcalophilus.'' This genus comprises species formerly belonging to the genus ''Bacillus'', a genus that has been recognized as displaying extensive polyphyly and phylogenetic heterogeneity due to the vague criteria (such as the ability to form endospores in the presence of oxygen) previously used to assign species to this clade. Multiple studies using comparative phylogenetic analyses have been published in an attempt to clarify the evolutionary relationships between ''Bacillus'' species, resulting in the establishment of numerous novel genera such as ''Alkalihalobacillus'', ''Brevibacillus, Solibacillus'', '' Alicyclobacillus'' and '' Virgibacillus''. The genus ''Bacillus'' is now restricted to species closely related to ''Bacillus subtilis'' and '' Bacillus cereus''. The na ...
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Tsukuba
is a city located in Ibaraki Prefecture, Japan. As of January 1, 2024, the city had an estimated population of 256,526 in 121,001 households and a population density of 900 persons per km2. The percentage of the population aged over 65 was 20.3%. The total area of the city is . It is known as the location of the , a planned science park developed in the 1960s. Geography Tsukuba is located in southern Ibaraki Prefecture, approximately 50 kilometers from central Tokyo and about 40 kilometers from Narita International Airport. Mount Tsukuba, from which the city takes its name, is located in the northern part of the city. Except for the area around Mount Tsukuba, the city is a part of the Kantō Plain with an altitude of 20 to 30 meters. Mountains: Mount Tsukuba, Mount Hokyo. Rivers: Kokai River, Sakura River, Higashiyata River, Nishiyata River, Ono River, Hanamuro River, Inari River. Parks: The city has more than 100 parks and green areas to relax in. Different parks a ...
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Bacillaceae
Bacillaceae, from Latin "bacillus", meaning "little staff, wand", are a family of gram-positive, heterotrophic, rod-shaped bacteria that may produce endospores. Motile members of this family are characterized by peritrichous flagella. Some Bacillaceae are aerobic, while others are facultative or strict anaerobes. Most are not pathogenic, but ''Bacillus'' species are known to cause disease in humans. Gram-variable cell wall Some Bacillaceae, such as the genera '' Filobacillus, Lentibacillus,'' and '' Halobacillus'', stain Gram-negative or Gram-variable, but are known to have a Gram-positive cell wall.Lim, J.M., Jeon, C.O., Song, S.M., and C.J. Kim. 2005''Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea'' Int. J. Syst. Evol. Microbiol. 55:165-170. Nomenclature Taxa within this family are sometimes colloquially identified as "bacilli". However, this term is ambiguous because it does not distinguish ...
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