BRB-seq
Bulk RNA barcoding and sequencing (BRB-seq) is an ultra-high-throughput bulk 3' mRNA-seq technology that uses early-stage sample barcoding and Unique molecular identifier, unique molecular identifiers (UMIs) to allow the pooling of up to 384 samples in one tube early in the sequencing library preparation workflow. The transcriptomic technology is compatible with both Illumina, Inc., Illumina and MGI (company), MGI short-read sequencing instruments. In standard RNA-Seq, RNA-seq, a sequencing library must be prepared for each RNA sample individually. In contrast, in BRB-seq, all samples are pooled early in the workflow for simultaneous processing to reduce the cost and hands-on time associated with the library preparation stage As BRB-seq is a 3' mRNA-seq technique, Short-read sequencing, short reads are generated only for the 3' region of polyadenylated mRNA molecules instead of the full length of transcripts like in standard RNA-Seq, RNA-seq. This means that BRB-seq requires a fa ...
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