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Atomic Fluorescence Spectroscopy
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy. In the special case of single molecule fluorescence spectroscopy, intensity fluctuations from the emitted light are measured from either single fluorophores, or pairs of fluorophores. Devices that measure fluorescence are called fluorometers. Theory Molecules have various states referred to as energy levels. Fluorescence spectroscopy is primarily concerned with electronic and vibrational states. Generally, the species being examined has a ground electronic state (a low energy state) of interest, and an excited electronic state of higher energy. Within each of these elect ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
PSA 2
PSA, PsA, Psa, or psa may refer to: Biology and medicine * Posterior spinal artery * Primary systemic amyloidosis, a disease caused by the accumulation of abnormal proteins * Prostate-specific antigen, an enzyme used as a blood tracer for prostate cancer * Psoriatic arthritis, an inflammatory disease * ''Pseudomonas aeruginosa'', a species of bacterium * Pseudomonas syringae pv. actinidiae, ''Pseudomonas syringae'' pv. ''actinidiae'', a pathovar of a bacterium that attacks kiwifruit Chemistry * Polar surface area, the surface sum over all polar atoms of a molecule * Pressure swing adsorption, a technology for separating, or purifying, gases Computing * Professional services automation, software for automating project and billing management for professional service firms * PSA Certified, Platform Security Architecture, a security certification for the Internet of Things * Plesk Server Administrator, a commercial web hosting automation program * Persistent staging area, a st ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Monochromator
A monochromator is an optics, optical device that transmits a mechanically selectable narrow band of wavelengths of light or other radiation chosen from a wider range of wavelengths available at the input. The name is . Uses A device that can produce monochromatic light has many uses in science and in optics because many optical characteristics of a material are dependent on wavelength. Although there are a number of useful ways to select a narrow band of wavelengths (which, in the visible range, is perceived as a pure color), there are not as many other ways to easily select any wavelength band from a wide range. See #Applications, below for a discussion of some of the uses of monochromators. In hard X-ray and neutron radiation, neutron optics, crystal monochromators are used to define wave conditions on the instruments. Techniques A monochromator can use either the phenomenon of optical dispersion in a Prism (optics), prism, or that of diffraction using a diffraction gratin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Virtual State (physics)
In quantum physics, a virtual state is a very short-lived, unobservable quantum state. In many quantum processes a virtual state is an intermediate state, sometimes described as "imaginary" in a multi-step process that mediates otherwise forbidden transitions. Since virtual states are not eigenfunctions of any operator, normal parameters such as occupation, energy and lifetime need to be qualified. No measurement of a system will show one to be occupied, but they still have lifetimes derived from uncertainty relations. While each virtual state has an associated energy, no direct measurement of its energy is possible but various approaches have been used to make some measurements (for example see and related work on virtual state spectroscopy) or extract other parameters using measurement techniques that depend upon the virtual state's lifetime. The concept is quite general and can be used to predict and describe experimental results in many areas including Raman spectroscopy, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Raman Scattering
In chemistry and physics, Raman scattering or the Raman effect () is the inelastic scattering of photons by matter, meaning that there is both an exchange of energy and a change in the light's direction. Typically this effect involves vibrational energy being gained by a molecule as incident photons from a visible laser are shifted to lower energy. This is called ''normal Stokes-Raman scattering''. Light has a certain probability of being scattered by a material. When photons are scattered, most of them are elastic scattering, elastically scattered (Rayleigh scattering), such that the scattered photons have the same energy (frequency, wavelength, and therefore color) as the incident photons, but different direction. Rayleigh scattering usually has an intensity in the range 0.1% to 0.01% relative to that of a radiation source. An even smaller fraction of the scattered photons (about 1 in a million) can be scattered ''inelastically'', with the scattered photons having an energy di ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rayleigh Scattering
Rayleigh scattering ( ) is the scattering or deflection of light, or other electromagnetic radiation, by particles with a size much smaller than the wavelength of the radiation. For light frequencies well below the resonance frequency of the scattering medium (normal dispersion relation, dispersion regime), the amount of scattering is inversely proportional to the fourth power of the wavelength (e.g., a blue color is scattered much more than a red color as light propagates through air). The phenomenon is named after the 19th-century British physicist Lord Rayleigh (John William Strutt). Rayleigh scattering results from the electric polarizability of the particles. The oscillating electric field of a light wave acts on the charges within a particle, causing them to move at the same frequency. The particle, therefore, becomes a small radiating dipole whose radiation we see as scattered light. The particles may be individual atoms or molecules; it can occur when light travels throu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorophore
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to macromolecules, serving as a markers (or dyes, or tags, or reporters) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, such as fluorescent imaging and spectroscopy. Fluorescein, via its amine-reactive isothiocyanate derivative fluorescein isothiocyanate (FITC), has been one of the most popular fluorophores ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Concentration
In chemistry, concentration is the abundance of a constituent divided by the total volume of a mixture. Several types of mathematical description can be distinguished: '' mass concentration'', '' molar concentration'', '' number concentration'', and '' volume concentration''. The concentration can refer to any kind of chemical mixture, but most frequently refers to solutes and solvents in solutions. The molar (amount) concentration has variants, such as normal concentration and osmotic concentration. Dilution is reduction of concentration, e.g. by adding solvent to a solution. The verb to concentrate means to increase concentration, the opposite of dilute. Etymology ''Concentration-'', ''concentratio'', action or an act of coming together at a single place, bringing to a common center, was used in post-classical Latin in 1550 or earlier, similar terms attested in Italian (1589), Spanish (1589), English (1606), French (1632). Qualitative description Often in informal, non- ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Intensity (physics)
In physics and many other areas of science and engineering the intensity or flux of radiant energy is the Power (physics), power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy. In the SI system, it has units watts per square metre (W/m2), or kilogram, kg⋅second, s−3 in SI base unit, base units. Intensity is used most frequently with waves such as acoustic waves (sound), matter waves such as electrons in electron microscopes, and electromagnetic waves such as light or radio waves, in which case the time averaging, ''average'' power transfer over one Period (physics), period of the wave is used. ''Intensity'' can be applied to other circumstances where energy is transferred. For example, one could calculate the intensity of the kinetic energy carried by drops of water from a garden sprinkler. The word "intensity" as used here is not synonymous with "wikt:strength, strength", "wikt:amplitude, amplitude ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Stray Light
Stray light is light in an optical system which was not intended in the design. The light may be from the intended source, but follow paths other than intended, or it may be from a source other than that intended. This light will often set a working limit on the dynamic range of the system; it limits the signal-to-noise ratio or contrast ratio, by limiting how dark the system can be. Ocular straylight is stray light in the human eye. Optical systems Monochromatic light Optical measuring instruments that work with monochromatic light, such as spectrophotometers, define stray light as light in the system at wavelengths (colors) other than the one intended. The stray light level is one of the most critical specifications of an instrument. For instance, intense, narrow absorption bands can easily appear to have a peak absorption less than the true absorption of the sample because the ability of the instrument to measure light transmission through the sample is limited by the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Collimated
A collimated beam of light or other electromagnetic radiation has parallel rays, and therefore will spread minimally as it propagates. A laser beam is an archetypical example. A perfectly collimated light beam, with no divergence, would not disperse with distance. However, diffraction prevents the creation of any such beam. Light can be approximately collimated by a number of processes, for instance by means of a collimator. Perfectly collimated light is sometimes said to be ''focused at infinity''. Thus, as the distance from a point source increases, the spherical wavefronts become flatter and closer to plane waves, which are perfectly collimated. Other forms of electromagnetic radiation can also be collimated. In radiology, X-rays are collimated to reduce the volume of the patient's tissue that is irradiated, and to remove stray photons that reduce the quality of the x-ray image ("film fog"). In scintigraphy, a gamma ray collimator is used in front of a detector to allow only ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
