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Agrobacterium Skierniewicense
''Agrobacterium'' is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. ''Agrobacterium tumefaciens'' is the most commonly studied species in this genus. ''Agrobacterium'' is well known for its ability to transfer DNA between itself and plants, and for this reason it has become an important tool for genetic engineering. Nomenclatural history Leading up to the 1990s, the genus ''Agrobacterium'' was used as a wastebasket taxon. With the advent of 16S sequencing, many ''Agrobacterium'' species (especially the marine species) were reassigned to genera such as '' Ahrensia'', '' Pseudorhodobacter'', ''Ruegeria'', and '' Stappia''. The remaining ''Agrobacterium'' species were assigned to three biovars: biovar 1 (''Agrobacterium tumefaciens''), biovar 2 (''Agrobacterium rhizogenes''), and biovar 3 (''Agrobacterium vitis''). In the early 2000s, ''Agrobacterium'' was synonymized with the genus ''Rhizobium''. This move ...
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Agrobacterium Radiobacter
''Agrobacterium radiobacter'' is the type species of the genus ''Agrobacterium'', a genus of Gram-negative bacteria that cause tumors in plants. It was incorrectly synonymized with ''Agrobacterium tumefaciens'' until 2022. This species is widely found in soil, in plant rhizospheres, and in human clinical specimens. Some members such as B6 harbor a tumor-inducing (Ti) plasmid and cause crown gall disease in plants. The type strain ATCC 19358 does not carry such a plasmid and is nonpathogenic. The incorrect synonimization with ''A. tumefaciens'' was due to a strain B6 (ATCC 23308) being assigned as the type strain of ''A. tumefaciens'' in 1980, when B6 should be classified as ''A. radiobacter''. One consequence is that this B6 strain is very well-studied in the lab. Another consequence is that research done on "''A. tumefaciens''" between 1980 and 2022 are possibly describing ''A. radiobacter'' instead. To determine the actual species, one should look up the 16S or genomic sequenc ...
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Harold J
Harold may refer to: People * Harold (given name), including a list of persons and fictional characters with the name * Harold (surname), surname in the English language * András Arató, known in meme culture as "Hide the Pain Harold" Arts and entertainment * ''Harold'' (film), a 2008 comedy film * ''Harold'', an 1876 poem by Alfred, Lord Tennyson * ''Harold, the Last of the Saxons'', an 1848 book by Edward Bulwer-Lytton, 1st Baron Lytton * '' Harold or the Norman Conquest'', an opera by Frederic Cowen * ''Harold'', an 1885 opera by Eduard Nápravník * Harold, a character from the cartoon ''The Grim Adventures of Billy & Mandy'' * Harold & Kumar, a US movie; Harold/Harry is the main actor in the show. Places ;In the United States * Alpine, Los Angeles County, California, an erstwhile settlement that was also known as Harold * Harold, Florida, an unincorporated community * Harold, Kentucky, an unincorporated community * Harold, Missouri, an unincorporated commun ...
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Synapomorphy
In phylogenetics, an apomorphy (or derived trait) is a novel Phenotypic trait, character or character state that has evolution, evolved from its ancestral form (or Plesiomorphy and symplesiomorphy, plesiomorphy). A synapomorphy is an apomorphy shared by two or more taxon, taxa and is therefore Hypothesis#Scientific hypothesis, hypothesized to have evolved in their most recent common ancestor. ) In cladistics, synapomorphy implies Homology (biology), homology. Examples of apomorphy are the presence of Terrestrial locomotion#Posture, erect gait, fur, Evolution of mammalian auditory ossicles, the evolution of three middle ear bones, and mammary glands in mammals but not in other vertebrate animals such as amphibians or reptiles, which have retained their ancestral traits of a Terrestrial locomotion#Posture, sprawling gait and lack of fur. Thus, these derived traits are also synapomorphies of mammals in general as they are not shared by other vertebrate animals. Etymology The word ...
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Monophyly
In biological cladistics for the classification of organisms, monophyly is the condition of a taxonomic grouping being a clade – that is, a grouping of organisms which meets these criteria: # the grouping contains its own most recent common ancestor (or more precisely an ancestral population), i.e. excludes non-descendants of that common ancestor # the grouping contains all the descendants of that common ancestor, without exception Monophyly is contrasted with paraphyly and polyphyly as shown in the second diagram. A ''paraphyletic'' grouping meets 1. but not 2., thus consisting of the descendants of a common ancestor, excepting one or more monophyletic subgroups. A ''polyphyletic'' grouping meets neither criterion, and instead serves to characterize convergent relationships of biological features rather than genetic relationships – for example, night-active primates, fruit trees, or aquatic insects. As such, these characteristic features of a polyphyletic grouping are ...
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Rhizobium
''Rhizobium'' is a genus of Gram-negative soil bacteria that fix nitrogen. ''Rhizobium'' species form an endosymbiotic nitrogen-fixing association with roots of (primarily) legumes and other flowering plants. The bacteria colonize plant cells to form root nodules, where they convert atmospheric nitrogen into ammonia using the enzyme nitrogenase. The ammonia is shared with the host plant in the form of organic nitrogenous compounds such as glutamine or ureides. The plant, in turn, provides the bacteria with organic compounds made by photosynthesis. This mutually beneficial relationship is true of all of the rhizobia, of which the genus ''Rhizobium'' is a typical example. ''Rhizobium'' is also capable of solubilizing phosphate. History Martinus Beijerinck was the first to isolate and cultivate a microorganism from the nodules of legumes in 1888. He named it ''Bacillus radicicola'', which is now placed in '' Bergey's Manual of Determinative Bacteriology'' under the gen ...
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Stappia
''Stappia'' is a genus of bactera in the order Hyphomicrobiales The Hyphomicrobiale' (synonym Rhizobiales) are an order of Gram-negative Alphaproteobacteria. The rhizobia, which fix nitrogen and are symbiotic with plant roots, appear in several different families. The four families '' Nitrobacteraceae'', ' .... Some members of the genus (now transferred to '' Labrenzia'') oxidize carbon monoxide (CO) aerobically. Stappia indica is a diatom associated bacterium which is known to inhibit the growth of diatoms such as Thalassiosira pseudonana. References Further reading Scientific journals * * Scientific books * Bacteria genera Hyphomicrobiales {{Hyphomicrobiales-stub ...
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Ruegeria
''Ruegeria'' is a genus of bacteria in the family Rhodobacteraceae. This genus was formerly known as the marine ''Agrobacterium'' before they were reclassified in 1998. It bears in fact the name of Hans-Jürgen Rüger, a German microbiologist, for his contribution to the taxonomy of marine species of ''Agrobacterium''. Characteristics The genus is characterised by members who are: * Gram-negative (like all Proteobacteria) * ovoid to rod-shaped cells 0.6–1.6 × 1.0–4.0 μm * motile by polar flagella, or nonmotile * non-sporeformers * aerobic * oxidase and catalase positive * are incapable of photosynthetic growth * bacteriochlorophyll a is absent * major quinone is ubiquinone 10 * GC-content, mol% G+C of the DNA is 55–59 * member of the family ''Rhodobacteraceae'', which is phenotypically, metabolically, ecologically diverse and defined based on 16S sequences data Phylogenetically it is very close to the genus ''Silicibacter'', but the two remain separate species due to ...
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Pseudorhodobacter
''Pseudorhodobacter'' is a genus of bacteria in the family Rhodobacteraceae.See the NCBI The National Center for Biotechnology Information (NCBI) is part of the National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH). It is approved and funded by the government of the United States. The NCBI is loca ...br>webpage on Pseudorhodobacter Data extracted from the References Further reading * Rhodobacteraceae Bacteria genera {{Rhodobacterales-stub ...
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Ahrensia
''Ahrensia'' is a genus of bacteria in the order Hyphomicrobiales.See the NCBIbr>webpage on Ahrensia Data extracted from the ''Ahrensia'' is named after the German microbiologist R. Ahrens. The cells are rod-shaped and motile. They are strictly aerobic.Garrity, George M.; Brenner, Don J.; Krieg, Noel R.; Staley, James T. (eds.) (2005). Bergey's Manual of Systematic Bacteriology, Volume Two: The Proteobacteria, Part C: The Alpha-, Beta-, Delta-, and Epsilonproteobacteria. New York, New York: Springer. . See also * List of bacterial genera named after personal names Many bacterial species are named after people, either the discoverer or a famous person in the field of microbiology. For example, ''Salmonella'' is named after D.E. Salmon, who discovered it (albeit as "''Bacillus typhi''"). For the generic epit ... References Further reading * Hyphomicrobiales Bacteria genera {{Hyphomicrobiales-stub ...
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16S Ribosomal RNA
16S ribosomal RNA (or 16 S rRNA) is the RNA component of the 30S subunit of a prokaryotic ribosome ( SSU rRNA). It binds to the Shine-Dalgarno sequence and provides most of the SSU structure. The genes coding for it are referred to as 16S rRNA genes and are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. Carl Woese and George E. Fox were two of the people who pioneered the use of 16S rRNA in phylogenetics in 1977. Multiple sequences of the 16S rRNA gene can exist within a single bacterium. Terminology The descriptor ''16S'' refers to the size of these ribosomal subunits as reflected indirectly by the speed at which they sediment when samples are centrifuged. Thus ''16S'' means 16 Svedburg units. Functions * Like the large (23S) ribosomal RNA, it has a structural role, acting as a scaffold defining the positions of the ribosomal proteins. * The 3-end contains the anti- Shine-Dalgarno sequence, which binds upstream ...
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Wastebasket Taxon
Wastebasket taxon (also called a wastebin taxon, dustbin taxon or catch-all taxon) is a term used by some taxonomists to refer to a taxon that has the purpose of classifying organisms that do not fit anywhere else. They are typically defined by either their designated members' often superficial similarity to each other, or their ''lack'' of one or more distinct character states or by their ''not'' belonging to one or more other taxa. Wastebasket taxa are by definition either paraphyletic or polyphyletic, and are therefore not considered valid taxa under strict cladistic rules of taxonomy. The name of a wastebasket taxon may in some cases be retained as the designation of an evolutionary grade, however. Examples There are many examples of paraphyletic groups, but true "wastebasket" taxa are those that are known not to, and perhaps not intended to, represent natural groups, but are nevertheless used as convenient groups of organisms. The acritarchs are perhaps the most famous ex ...
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Genetic Engineering
Genetic engineering, also called genetic modification or genetic manipulation, is the modification and manipulation of an organism's genes using technology. It is a set of Genetic engineering techniques, technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating and copying the genetic material of interest using recombinant DNA methods or by Artificial gene synthesis, artificially synthesising the DNA. A Vector (molecular biology), construct is usually created and used to insert this DNA into the host organism. The first recombinant DNA molecule was made by Paul Berg in 1972 by combining DNA from the monkey virus SV40 with the Lambda phage, lambda virus. As well as inserting genes, the process can be used to remove, or "Gene knockout, knock out", genes. The new DNA can either be inserted randomly or Gene targeting, targeted to a spe ...
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