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Agglomerin
Agglomerins are bacterial natural products, identified as metabolites of ''Pantoea agglomerans'' which was isolated in 1989 from river water in Kobe, Japan. They belong to the class of tetronate antibiotics, which include tetronomycin, tetronasin, and Atrop-abyssomicin C, abyssomicin C. The members of the agglomerins differ only in the composition of the acyl chain attached to the tetronate ring. They possess antibiotic activity against anaerobic bacteria and weak activity against aerobic bacteria ''in vitro''. The structures were solved in 1990. Agglomerin A is the major component (38%), followed by agglomerin B (30%), agglomerin C (24%), and agglomerin D (8%). Biosynthesis The biosynthetic gene cluster for agglomerins is 12 kb, and codes for 7 open reading frames. The glyceryl-S-ACP is derived from D-1,3-bisphosphoglycerate by Agg2 (glyceryl-''S''-ACP synthase) and Agg3 (acyl carrier protein). The acyl chain is taken from primary metabolism as a 3-oxoacyl-CoA thioester. The ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Atrop-abyssomicin C
''Atrop''-abyssomicin C is a polycyclic polyketide-type natural product that is the atropisomer of abyssomicin C. It is a spirotetronate that belongs to the class of tetronate antibiotics, which includes compounds such as tetronomycin, agglomerin, and chlorothricin. In 2006, the Nicolaou group discovered ''atrop''-abyssomicin C while working on the total synthesis of abyssomicin C. Then in 2007, Süssmuth and co-workers isolated ''atrop''-abyssomicin C from '' Verrucosispora maris'' AB-18-032, a marine actinomycete found in sediment of the Japanese sea. They found that ''atrop''-abyssomicin C was the major metabolite produced by this strain, while abyssomicin C was a minor product. The molecule displays antibacterial activity by inhibiting the enzyme PabB ( 4-amino-4-deoxychorismate synthase), thereby depleting the biosynthesis of '' p'' -aminobenzoate. Structure ''Atrop''-abyssomicin C has a complex, yet intriguing structural topography. The compound contains an oxabicycl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic re ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Natural Product
A natural product is a natural compound or substance produced by a living organism—that is, found in nature. In the broadest sense, natural products include any substance produced by life. Natural products can also be prepared by chemical synthesis (both semisynthesis and total synthesis) and have played a central role in the development of the field of organic chemistry by providing challenging synthetic targets. The term natural product has also been extended for commercial purposes to refer to cosmetics, dietary supplements, and foods produced from natural sources without added artificial ingredients. Within the field of organic chemistry, the definition of natural products is usually restricted to organic compounds isolated from natural sources that are produced by the pathways of primary or secondary metabolism. Within the field of medicinal chemistry, the definition is often further restricted to secondary metabolites. Secondary metabolites (or specialized metabo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Metabolite
In biochemistry, a metabolite is an intermediate or end product of metabolism. The term is usually used for small molecules. Metabolites have various functions, including fuel, structure, signaling, stimulatory and inhibitory effects on enzymes, catalytic activity of their own (usually as a cofactor to an enzyme), defense, and interactions with other organisms (e.g. pigments, odorants, and pheromones). A primary metabolite is directly involved in normal "growth", development, and reproduction. Ethylene exemplifies a primary metabolite produced large-scale by industrial microbiology. A secondary metabolite is not directly involved in those processes, but usually has an important ecological function. Examples include antibiotics and pigments such as resins and terpenes etc. Some antibiotics use primary metabolites as precursors, such as actinomycin, which is created from the primary metabolite tryptophan. Some sugars are metabolites, such as fructose or glucose, which ar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pantoea Agglomerans
''Pantoea agglomerans'' is a Gram-negative bacterium that belongs to the family Erwiniaceae. It was formerly called ''Enterobacter agglomerans'', or ''Erwinia herbicola'' and is a ubiquitous bacterium commonly isolated from plant surfaces, seeds, fruit, and animal or human feces and can be found throughout a honeybee's environment. Levan produced by ''Pantoea agglomerans'' ZMR7 was reported to decrease the viability of rhabdomyosarcoma (RD) and breast cancer (MDA) cells compared with untreated cancer cells. In addition, it has high antiparasitic activity against the promastigote of '' Leishmania tropica''. Plant Disease Biocontrol ''Pantoea agglomerans'' can serve as a biocontrol organism for the management of plant diseases. It has been used to control fire blight, a plant disease caused by bacterium '' Erwinia amylovora,'' that is a common problem in pear and apple crops. After coming in contact with ''Erwinia amylovora'', ''Pantoea agglomerans'' produces antibiotic compound ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Kobe
Kobe ( , ; officially , ) is the capital city of Hyōgo Prefecture Japan. With a population around 1.5 million, Kobe is Japan's seventh-largest city and the third-largest port city after Tokyo and Yokohama. It is located in Kansai region, which makes up the southern side of the main island of Honshū, on the north shore of Osaka Bay. It is part of the Keihanshin metropolitan area along with Osaka and Kyoto. The Kobe city centre is located about west of Osaka and southwest of Kyoto. The earliest written records regarding the region come from the '' Nihon Shoki'', which describes the founding of the Ikuta Shrine by Empress Jingū in AD 201.Ikuta Shrine official website – "History of Ikuta Shrine" (Japanese) [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Japan
Japan ( ja, 日本, or , and formally , ''Nihonkoku'') is an island country in East Asia. It is situated in the northwest Pacific Ocean, and is bordered on the west by the Sea of Japan, while extending from the Sea of Okhotsk in the north toward the East China Sea, Philippine Sea, and Taiwan in the south. Japan is a part of the Ring of Fire, and spans an archipelago of 6852 islands covering ; the five main islands are Hokkaido, Honshu (the "mainland"), Shikoku, Kyushu, and Okinawa. Tokyo is the nation's capital and largest city, followed by Yokohama, Osaka, Nagoya, Sapporo, Fukuoka, Kobe, and Kyoto. Japan is the eleventh most populous country in the world, as well as one of the most densely populated and urbanized. About three-fourths of the country's terrain is mountainous, concentrating its population of 123.2 million on narrow coastal plains. Japan is divided into 47 administrative prefectures and eight traditional regions. The Greater Tokyo Ar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
In Vitro
''In vitro'' (meaning in glass, or ''in the glass'') studies are performed with microorganisms, cells, or biological molecules outside their normal biological context. Colloquially called "test-tube experiments", these studies in biology and its subdisciplines are traditionally done in labware such as test tubes, flasks, Petri dishes, and microtiter plates. Studies conducted using components of an organism that have been isolated from their usual biological surroundings permit a more detailed or more convenient analysis than can be done with whole organisms; however, results obtained from ''in vitro'' experiments may not fully or accurately predict the effects on a whole organism. In contrast to ''in vitro'' experiments, '' in vivo'' studies are those conducted in living organisms, including humans, and whole plants. Definition ''In vitro'' ( la, in glass; often not italicized in English usage) studies are conducted using components of an organism that have been isolated f ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Open Reading Frame
In molecular biology, open reading frames (ORFs) are defined as spans of DNA sequence between the start and stop codons. Usually, this is considered within a studied region of a Prokaryote, prokaryotic DNA sequence, where only one of the #Six-frame translation, six possible reading frames will be "open" (the "reading", however, refers to the RNA produced by Transcription (biology), transcription of the DNA and its subsequent interaction with the ribosome in Translation (biology), translation). Such an ORF may contain a start codon (usually AUG in terms of RNA) and by definition cannot extend beyond a stop codon (usually UAA, UAG or UGA in RNA). That start codon (not necessarily the first) indicates where translation may start. The transcription terminator, transcription termination site is located after the ORF, beyond the Translation (biology), translation stop codon. If transcription were to cease before the stop codon, an incomplete protein would be made during translation. In ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
