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Acid-fast Staining
Acid-fastness is a physical property of certain bacterial and eukaryotic cells, as well as some sub-cellular structures, specifically their resistance to decolorization by acids during laboratory staining procedures. Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name ''acid-fast''. The mechanisms of acid-fastness vary by species although the most well-known example is in the genus ''Mycobacterium'', which includes the species responsible for tuberculosis and leprosy. The acid-fastness of ''Mycobacteria'' is due to the high mycolic acid content of their cell walls, which is responsible for the staining pattern of poor absorption followed by high retention. Some bacteria may also be partially acid-fast, such as ''Nocardia''. Acid-fast organisms are difficult to characterize using standard microbiological techniques, though they can be stained using concentrated dyes, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mycobacterium Tuberculosis Ziehl-Neelsen Stain 640
''Mycobacterium'' is a genus of over 190 species in the phylum Actinomycetota, assigned its own family, Mycobacteriaceae. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (''Mycobacterium tuberculosis, M. tuberculosis'') and leprosy (''Mycobacterium leprae, M. leprae'') in humans. The Greek language, Greek prefix ''myco-'' means 'fungus', alluding to this genus' Mold (fungus), mold-like colony surfaces. Since this genus has cell walls with a waxy lipid-rich outer layer containing high concentrations of mycolic acid, acid-fast staining is used to emphasize their resistance to acids, compared to other cell types. Mycobacterial species are generally aerobic, non-motile, and capable of growing with minimal nutrition. The genus is divided based on each species' pigment production and growth rate. While most ''Mycobacterium'' species are non-pathogenic, the genus' characteristic complex cell wall contributes to evasion from host defenses. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Auramine Phenol Stain
Auramine phenol stain is a stain used in clinical microbiology and histology to identify tuberculosis mycobacteria. There are two types of auramine phenol stains, 1 and 2 to stain mycobacterium species and cryptosporidium respectively. Both are fluorescent Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation. When exposed to ultraviolet radiation, many substances will glow (fluoresce) with color ... stains. The bacteria or the parasites appear brilliant greenish yellow against dark background. Mycolic acids of the mycobacteria keep this stain when decolorising with the acid alcohol. The method is more rapid and sensitive than ZN technique. Method * Smears are prepared just like that for ZN staining * Stain with Auramine-Phenol for 20 mins * Rinse with water * Decolourise in acid alcohol * Rinse with water * Counterstain with 0.1% potassium permanganate for 30 seconds * Rinse ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ellis And Zabrowarny Stain
Ellis is a surname of Welsh and English origin. Retrieved 21 January 2014 An independent French origin of the surname is said to derive from the phrase fleur-de-lis. It has also been noted to be a Jewish surname. People with the surname include: A * Adam Ellis, American webcomic artist * Adam Ellis (born 1996), British grasstrack and speedway rider * Adam Gibb Ellis, Chief Justice of Jamaica * Adrienne Ellis (born 1944), American-Canadian actress * Albert Ellis (other), multiple people * Alexander Ellis (other), multiple people * Allan Ellis (other) * Alton Ellis (1938–2008), Jamaican musician * Andrew Ellis (other), multiple people * Anita Ellis, Canadian-born American singer and actress * Annette Ellis (born 1946), Australian politician * Arthur Ellis (other), multiple people * Atom Ellis (born 1966), American musician *Aunjanue Ellis (born 1969), American actress B * Ben Ellis (other), multiple people * Bill Ellis (191 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Wade Fite Stain
Wade, WADE, or Wades may refer to: Places in the United States * Wade, Maine, a town * Wade, Mississippi, a census-designated place * Wade, North Carolina, a town * Wade, Ohio, an unincorporated community * Wade Township, Clinton County, Illinois * Wade Township, Jasper County, Illinois * Wade County, Choctaw Nation, a former political subdivision * Wades Branch, a river in Tennessee People, fictional characters and mythological creatures * Wade (given name), a list of people and fictional characters * Wade (surname), including a list of people and fictional characters * Wade (folklore), a being from Germanic mythology and folklore Other uses * ''Wade'' (film), a 2020 Indian animated short film * World Alliance for Decentralized Energy (WADE) * Wade Ceramics, manufacturers of porcelain and earthenware; known for making "Whimsies" * WADE (AM), a radio station in Wadesboro, North Carolina, United States * Wade–Giles, a method of Romanisation of Chinese, sometimes a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fite Stain
Fite may refer to: People * Bobby Fite (born 1968), American film and television actor * Charlene Fite (born 1950), American politician * Gilbert Fite (1918–2010), American historian * Harvey Fite (1903–1976), American sculptor and painter * Lanny Fite (born c. 1949), American politician * Lea Fite (1955–2009), American state legislator from Alabama * Linda Fite, American comics writer and editor * Mark Fite, American actor and comedian * Nina Maria Fite, American diplomat *Rankin Fite (1916–1980), American state legislator and attorney * Samuel McClary Fite (1816–1875), American politician *Tim Fite, American musician * Warner Fite (1867–1955), American philosopher * Wendell Fite (born 1965), American DJ, producer and rapper known as DJ Hurricane Other uses * Anita Fite, a fictional character in DC Comics * Fite, Republic of Dagestan, Russia * FITE TV, a video streaming service for ring sports live programming * Henry Fite House The "Henry Fite House", located ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Polyglycol Ether
Glycol ethers are a class of chemical compounds consisting of alkyl ethers that are based on glycols such as ethylene glycol or propylene glycol. They are commonly used as solvents in paints and cleaners. They have good solvent properties while having higher boiling points than the lower-molecular-weight ethers and alcohols. History The name "Cellosolve" was registered in 1924 as a United States trademark by Carbide & Carbon Chemicals Corporation (a division of Union Carbide Corporation) for "Solvents for Gums, Resins, Cellulose Esters, and the Like". "Ethyl Cellosolve" or simply "Cellosolve" consists mainly of ethylene glycol monoethyl ether and was introduced as a lower-cost solvent alternative to ethyl lactate. "Butyl Cellosolve" ( ethylene glycol monobutyl ether) was introduced in 1928, and "Methyl Cellosolve" ( ethylene glycol monomethyl ether) in 1929. Types Glycol ethers are designated "E-series" or "P-series" for those made from ethylene oxide or propylene oxide, resp ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Schaeffer–Fulton Stain
The Schaeffer–Fulton stain is a technique designed to isolate endospores by staining any present endospores green, and any other bacterial bodies red. The primary stain is malachite green, and the counterstain is safranin, which dyes any other bacterial bodies red. Endospores cannot be stained by normal staining procedures because their walls are practically impermeable to all chemicals. The Schaeffer- Fulton endospore stain uses heat to drive the primary stain(malachite green) into the endospore. After cooling, the slide is decolorized with water and counterstained with safranin. Procedure Using an aseptic technique, bacteria are placed on a slide and heat fixed. The slide is then suspended over a water bath with some sort of porus paper over it, so that the slide is steamed. Malachite green is applied to the slide, which can penetrate the tough walls of the endospores, staining them green. After five minutes, the slide is removed from the steam, and the paper towel is remove ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Moeller Stain
Moeller staining involves the use of a steamed dye reagent in order to increase the stainability of endospores. Carbol fuchsin is the primary stain used in this method. Endospores are stained red, while the counterstain methylene blue stains the vegetative bacteria blue. Endospores are surrounded by a highly resistant spore coat, which is highly resistant to excessive heat, freezing, desiccation, as well as chemical agents. More importantly, for identification, spores are resistant to commonly employed staining techniques; therefore alternative staining methods are required. Method Carbol fuchsin is applied to a heat-fixed slide. The slide is then heated over a bunsen burner, or suspended over a hot water bath, covered with a paper towel, and steamed for 3 minutes. The slide is rinsed with acidified ethanol, and counter-stained with Methylene blue. An improved method involves the addition of the surfactant Tergitol 7 to the carbol fuchsin stain, and the omission of the steaming ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methylene Blue
Methylthioninium chloride, commonly called methylene blue, is a salt used as a dye and as a medication. As a medication, it is mainly used to treat methemoglobinemia. It has previously been used for treating cyanide poisoning and urinary tract infections, but this use is no longer recommended. Methylene blue is typically given by injection into a vein. Common side effects include headache, nausea, and vomiting. Methylene blue was first prepared in 1876, by Heinrich Caro. It is on the World Health Organization's List of Essential Medicines. Medical uses Methemoglobinemia Methylene blue is used to treat methemoglobinemia by chemically reducing the ferric iron in hemoglobin to ferrous iron. Methemoglobinemia can arise from ingestion of certain pharmaceuticals, toxins, or broad beans in those susceptible. Specifically, it is used to treat methemoglobin levels that are greater than 30% or in which there are symptoms despite oxygen therapy. Normally, through the NADH- or ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
