α-hemolysis
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α-hemolysis
Hemolysis is the breakdown of red blood cells. The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms. This is particularly useful in classifying streptococcal species. A substance that causes hemolysis is called a hemolysin. Types Alpha-hemolysis When alpha-hemolysis (α-hemolysis) is present, the agar under the colony is light and greenish. ''Streptococcus pneumoniae'' and a group of oral streptococci ( ''Streptococcus'' viridans or viridans streptococci) display alpha-hemolysis. This is sometimes called ''green hemolysis'' because of the color change in the agar. Other synonymous terms are ''incomplete hemolysis'' and ''partial hemolysis''. Alpha-hemolysis is caused by the bacteria's production of hydrogen peroxide, which oxidizes hemoglobin and produces the green oxidized derivative methemoglobin. Beta-hemolysis Beta-hemolysis (β-hemolysis), sometimes called ''complete hemolysis'', is a complete lysis of re ...
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Streptococcal Hemolysis
''Streptococcus'' is a genus of gram-positive bacteria, gram-positive spherical bacteria that belongs to the family Streptococcaceae, within the order Lactobacillales (lactic acid bacteria), in the phylum Bacillota. Cell division in streptococci occurs along a single coordinate system, axis, thus when growing they tend to form pairs or chains, which may appear bent or twisted. This differs from Staphylococcus, staphylococci, which divide along multiple axes, thereby generating irregular, grape-like clusters of cell (biology), cells. Most streptococci are Oxidase test, oxidase-negative and Catalase#Clinical significance and application, catalase-negative, and many are facultative anaerobic organism, facultative anaerobes (capable of growth both aerobically and anaerobically). The term was coined in 1877 by Viennese surgeon Theodor Billroth, Albert Theodor Billroth (1829–1894), by combining the prefix "strepto-" (from ), together with the suffix "-coccus" (from Modern , from .) ...
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Blood Agar
An agar plate is a Petri dish that contains a growth medium solidified with agar, used to Microbiological culture, culture microorganisms. Sometimes selective compounds are added to influence growth, such as antibiotics. Individual microorganisms placed on the plate will grow into individual colony (biology), colonies, each a cloning, clone genetically identical to the individual ancestor organism (except for the low, unavoidable rate of mutation). Thus, the plate can be used either to estimate the concentration of organisms in a Microbiological culture, liquid culture or a suitable dilution of that culture using a colony counter, or to generate genetically pure cultures from a mixed culture of genetically different organisms. Several methods are available to plate out cells. One technique is known as "Streaking (microbiology), streaking". In this technique, a drop of the culture on the end of a thin, sterilization (microbiology), sterile loop of wire, sometimes known as an inocu ...
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Streptococcal
''Streptococcus'' is a genus of gram-positive spherical bacteria that belongs to the family Streptococcaceae, within the order Lactobacillales (lactic acid bacteria), in the phylum Bacillota. Cell division in streptococci occurs along a single axis, thus when growing they tend to form pairs or chains, which may appear bent or twisted. This differs from staphylococci, which divide along multiple axes, thereby generating irregular, grape-like clusters of cells. Most streptococci are oxidase-negative and catalase-negative, and many are facultative anaerobes (capable of growth both aerobically and anaerobically). The term was coined in 1877 by Viennese surgeon Albert Theodor Billroth (1829–1894), by combining the prefix "strepto-" (from ), together with the suffix "-coccus" (from Modern , from .) In 1984, many bacteria formerly grouped in the genus ''Streptococcus'' were separated out into the genera ''Enterococcus'' and '' Lactococcus''. Currently, over 50 species are recogn ...
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Streptococcus Agalactiae
''Streptococcus agalactiae'' (also known as group B streptococcus or GBS) is a gram-positive coccus (round bacterium) with a tendency to form chains (as reflected by the genus name ''Streptococcus''). It is a beta-hemolytic, catalase-negative, and facultative anaerobe. ''S. agalactiae'' is the most common human pathogen of streptococci belonging to group B of the Rebecca Lancefield classification of streptococci. GBS are surrounded by a bacterial capsule composed of polysaccharides (exopolysaccharide). The species is subclassified into ten serotypes (Ia, Ib, II–IX) depending on the immunologic reactivity of their polysaccharide capsule. The plural term group B streptococci (referring to the serotypes) and the singular term group B streptococcus (referring to the single species) are both commonly used synonymously with ''S. agalactiae'' even though '' S. halichoeri'' and '' S. pseudoporcinus'' are also group B Streptococci. These species test positive as group B, but are not fr ...
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International Standard Serial Number
An International Standard Serial Number (ISSN) is an eight-digit to uniquely identify a periodical publication (periodical), such as a magazine. The ISSN is especially helpful in distinguishing between serials with the same title. ISSNs are used in ordering, cataloging, interlibrary loans, and other practices in connection with serial literature. The ISSN system was first drafted as an International Organization for Standardization (ISO) international standard in 1971 and published as ISO 3297 in 1975. ISO subcommittee TC 46/SC 9 is responsible for maintaining the standard. When a serial with the same content is published in more than one media type, a different ISSN is assigned to each media type. For example, many serials are published both in print and electronic media. The ISSN system refers to these types as print ISSN (p-ISSN) and electronic ISSN (e-ISSN). Consequently, as defined in ISO 3297:2007, every serial in the ISSN system is also assigned a linking ISSN ...
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Digital Object Identifier
A digital object identifier (DOI) is a persistent identifier or handle used to uniquely identify various objects, standardized by the International Organization for Standardization (ISO). DOIs are an implementation of the Handle System; they also fit within the URI system ( Uniform Resource Identifier). They are widely used to identify academic, professional, and government information, such as journal articles, research reports, data sets, and official publications. A DOI aims to resolve to its target, the information object to which the DOI refers. This is achieved by binding the DOI to metadata about the object, such as a URL where the object is located. Thus, by being actionable and interoperable, a DOI differs from ISBNs or ISRCs which are identifiers only. The DOI system uses the indecs Content Model to represent metadata. The DOI for a document remains fixed over the lifetime of the document, whereas its location and other metadata may change. Referring to ...
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McGraw Hill
McGraw Hill is an American education science company that provides educational content, software, and services for students and educators across various levels—from K-12 to higher education and professional settings. They produce textbooks, digital learning tools, and adaptive technology to enhance learning experiences and outcomes. It is one of the "big three" educational publishers along with Houghton Mifflin Harcourt and Pearson Education. McGraw Hill also publishes reference and trade publications for the medical, business, and engineering professions. Formerly a division of The McGraw Hill Companies (later renamed McGraw Hill Financial, now S&P Global), McGraw Hill Education was divested and acquired by Apollo Global Management in March 2013 for $2.4 billion in cash. McGraw Hill was sold in 2021 to Platinum Equity for $4.5 billion. History McGraw Hill was founded in 1888, when James H. McGraw, co-founder of McGraw Hill, purchased the ''American Journal of Railwa ...
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Vibrio Cholerae
''Vibrio cholerae'' is a species of Gram-negative bacteria, Gram-negative, Facultative anaerobic organism, facultative anaerobe and Vibrio, comma-shaped bacteria. The bacteria naturally live in Brackish water, brackish or saltwater where they attach themselves easily to the chitin-containing shells of crabs, shrimp, and other shellfish. Some strains of ''V. cholerae'' are pathogenic to humans and cause a deadly disease called cholera, which can be derived from the consumption of undercooked or raw marine life species or drinking contaminated water. ''V. cholerae'' was first described by Félix-Archimède Pouchet in 1849 as some kind of protozoa. Filippo Pacini correctly identified it as a bacterium and from him, the scientific name is adopted. The bacterium as the cause of cholera was discovered by Robert Koch in 1884. Sambhu Nath De isolated the cholera toxin and demonstrated the toxin as the cause of cholera in 1959. The bacterium has a flagellum (a tail like structure) at one ...
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Staphylococcus Epidermidis
''Staphylococcus epidermidis'' is a Gram-positive bacterium, and one of over 40 species belonging to the genus ''Staphylococcus''. It is part of the human flora, normal human microbiota, typically the skin flora, skin microbiota, and less commonly the mucosal microbiota and also found in marine sponges. It is a facultative anaerobic bacteria. Although ''S. epidermidis'' is not usually Pathogenic bacteria, pathogenic, patients with compromised immune systems are at risk of developing infection. These infections are generally Hospital-acquired infection, hospital-acquired. ''S. epidermidis'' is a particular concern for people with catheters or other surgical implants because it is known to form biofilms that grow on these devices. Being part of the normal skin microbiota, ''S. epidermidis'' is a frequent contaminant of specimens sent to the diagnostic laboratory. Some strains of ''S. epidermidis'' are highly salt tolerant and commonly found in marine environments. S.I. Paul et al ...
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Staphylococcus Saprophyticus
''Staphylococcus saprophyticus'' is a Gram-positive coccus belonging to the genus ''Staphylococcus''. ''S. saprophyticus'' is a common cause of community-acquired urinary tract infections. History ''Staphylococcus saprophyticus'' was not recognized as a cause of urinary tract infections until the early 1970s, more than 10 years after its original demonstration in urine specimens. Prior to this, the presence of coagulase-negative staphylococci (CoNS) in urine specimens was dismissed as contamination. Epidemiology and pathogenesis In humans, ''S. saprophyticus'' is found in the normal flora of the female genital tract and perineum. It has been isolated from other sources, too, including meat and cheese products, vegetables, the environment, and human and animal gastrointestinal tracts. ''S. saprophyticus'' causes 10–20% of urinary tract infections (UTIs). In females 17–27 years old, it is the second-most common cause of community-acquired UTIs, after ''Escherichia coli''. ...
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Enterococcus Faecalis
''Enterococcus faecalis'' – formerly classified as part of the group D '' Streptococcus,'' is a Gram-positive, commensal bacterium naturally inhabiting the gastrointestinal tracts of humans. Like other species in the genus '' Enterococcus'', ''E. faecalis'' is found in healthy humans and can be used as a probiotic. The probiotic strains such as Symbioflor1 and EF-2001 are characterized by the lack of specific genes related to drug resistance and pathogenesis. Despite its commensal role, ''E. faecalis'' is an opportunistic pathogen capable of causing severe infections, especially in the nosocomial (hospital) settings. ''Enterococcus'' spp. is among the leading causes of healthcare-associated infections ranging from endocarditis to urinary tract infections (UTIs). Hospital-acquired UTIs are associated with catheterization because catheters provide an ideal surface for biofilm formation, allowing ''E. faecalis'' to adhere, persist, and evade both the immune response and antibiot ...
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Clostridium Perfringens
''Clostridium perfringens'' (formerly known as ''C. welchii'', or ''Bacillus welchii'') is a Gram-positive, bacillus (rod-shaped), anaerobic, spore-forming pathogenic bacterium of the genus '' Clostridium''. ''C. perfringens'' is ever-present in nature and can be found as a normal component of decaying vegetation, marine sediment, the intestinal tract of humans and other vertebrates, insects, and soil. It has the shortest reported generation time of any organism at 6.3 minutes in thioglycolate medium. ''Clostridium perfringens'' is one of the most common causes of food poisoning in the United States, alongside norovirus, ''Salmonella'', '' Campylobacter'', and ''Staphylococcus aureus''. However, it can sometimes be ingested and cause no harm. Infections induced by ''C. perfringens'' are associated with tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, which is also known as clostridial myonecrosis. The specific name, ''perfringens,'' is derived ...
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