Ion Semiconductor Sequencing

Ion semiconductor sequencing is a method of DNA sequencing based on the detection of hydrogen ions that are released during the polymerization of DNA. This is a method of "sequencing by synthesis", during which a complementary strand is built based on the sequence of a template strand.

A microwell containing a template DNA strand to be sequenced is flooded with a single species of deoxyribonucleotide triphosphate (dNTP). If the introduced dNTP is complementary to the leading template nucleotide, it is incorporated into the growing complementary strand. This causes the release of a hydrogen ion that triggers an ion-sensitive field-effect transistor (ISFET) sensor, which indicates that a reaction has occurred. If homopolymer repeats are present in the template sequence, multiple dNTP molecules will be incorporated in a single cycle. This leads to a corresponding number of released hydrogens and a proportionally higher electronic signal.

This technology differs from other sequencing-by-synthesis technologies in that no modified nucleotides or optics are used. Ion semiconductor sequencing may also be referred to as Ion Torrent sequencing, pH-mediated sequencing, silicon sequencing, or semiconductor sequencing.

Technology development history

The technology was licensed from DNA Electronics Ltd,Bio-IT World, Davies, K.
Powering Preventative Medicine
. Bio-IT World 2011GenomeWe
DNA Electronics Licenses IP to Ion Torrent
August 2010 developed by Ion Torrent Systems Inc. and was released in February 2010.Rusk, N. (2011)

Nat Meth 8(1): 44-44. Ion Torrent have marketed their machine as a rapid, compact and economical sequencer that can be utilized in a large number of laboratories as a bench top machine.Ion Torrent Official Webpage
. Roche's 454 Life Sciences is partnering with DNA Electronics on the development of a long-read, high-density semiconductor sequencing platform using this technology.GenomeWe
Roche Partners with DNA Electronics to Help Migrate 454 Platform to Electrochemical Detection
November 2010

Technology

Sequencing chemistry

In nature, the incorporation of a deoxyribonucleoside triphosphate (dNTP) into a growing DNA strand involves the formation of a covalent bond and the release of pyrophosphate and a positively charged hydrogen ion.Purushothaman, S, Toumazou, C, Ou, C-
Protons and single nucleotide polymorphism detection: a simple use for the ion sensitive field effect transistor
/ref> A dNTP will only be incorporated if it is complementary to the leading unpaired template nucleotide. Ion semiconductor sequencing exploits these facts by determining if a hydrogen ion is released upon providing a single species of dNTP to the reaction.

Microwells on a semiconductor chip that each contain many copies of one single-stranded template DNA molecule to be sequenced and DNA polymerase are sequentially flooded with unmodified A, C, G or T dNTP.Perkel, J.
"Making contact with sequencing's fourth generation"
. Biotechniques, 2011. If an introduced dNTP is complementary to the next unpaired nucleotide on the template strand it is incorporated into the growing complementary strand by the DNA polymerase.Alberts B
Molecular Biology of the Cell
5th Edition ed. 2008, New York: Garland Science. If the introduced dNTP is not complementary there is no incorporation and no biochemical reaction. The hydrogen ion that is released in the reaction changes the pH of the solution, which is detected by an ISFET. The unattached dNTP molecules are washed out before the next cycle when a different dNTP species is introduced.

Signal detection

Beneath the layer of microwells is an ion sensitive layer, below which is an ISFET ion sensor. All layers are contained within a CMOS semiconductor chip, similar to that used in the electronics industry.Karow, J. (2009
Ion Torrent Patent App Suggests Sequencing Tech Using Chemical-Sensitive Field-Effect Transistors
In Sequence.

Each chip contains an array of microwells with corresponding ISFET detectors.
Each released hydrogen ion then triggers the ISFET ion sensor. The series of electrical pulses transmitted from the chip to a computer is translated into a DNA sequence, with no intermediate signal conversion required.Bio-IT World, Davies, K

. Bio-IT World 2010. Because nucleotide incorporation events are measured directly by electronics, the use of labeled nucleotides and optical measurements are avoided. Signal processing and DNA assembly can then be carried out in software.

Sequencing characteristics

The per base accuracy achieved on th
Ion Torrent
Ion semiconductor sequencer as of February 2011 was 99.6% based on 50 base reads, with 100 Mb per run.Karow, J. (2009
At AGBT, Ion Torrent Customers Provide First Feedback; Life Tech Outlines Platform's Growth
In Sequence. The read-length as of February 2011 was 100 base pairs. The accuracy for homopolymer repeats of 5 repeats in length was 98%. Later releases show a read length of 400 base pairs These figures have not yet been independently verified outside of the company.

Strengths

The major benefits of ion semiconductor sequencing are rapid sequencing speed and low upfront and operating costs. This has been enabled by the avoidance of modified nucleotides and optical measurements.

Because the system records natural polymerase-mediated nucleotide incorporation events, sequencing can occur in real-time. In reality, the sequencing rate is limited by the cycling of substrate nucleotides through the system.Eid, J., et al.
"Real-time DNA sequencing from single polymerase molecules"
Science, 2009. 323(5910): p. 133-8. Ion Torrent Systems Inc., the developer of the technology, claims that each incorporation measurement takes 4 seconds and each run takes about one hour, during which 100-200 nucleotides are sequenced.Karow, J. (2010
Ion Torrent Systems Presents $50,000 Electronic Sequencer at AGBT
In Sequence. If the semiconductor chips are improved (as predicted by Moore’s law), the number of reads per chip (and therefore per run) should increase.

The cost of acquiring a pH-mediated sequencer at time of launch was priced at around $50,000 USD, excluding sample preparation equipment and a server for data analysis. The cost per run is also significantly lower than that of alternative automated sequencing methods, at roughly $1,000.

Limitations

If homopolymer repeats of the same nucleotide (e.g. ) are present on the template strand (strand to be sequenced) then multiple introduced nucleotides are incorporated and more hydrogen ions are released in a single cycle. This results in a greater pH change and a proportionally greater electronic signal. This is a limitation of the system in that it is difficult to enumerate long repeats. This limitation is shared by other techniques that detect single nucleotide additions such as pyrosequencing.Metzker, M.L.
"Emerging technologies in DNA sequencing"
Genome Res, 2005. 15(12): p. 1767-76. Signals generated from a high repeat number are difficult to differentiate from repeats of a similar but different number; ''e.g.'', homorepeats of length 7 are difficult to differentiate from those of length 8.

Another limitation of this system is the short read length compared to other sequencing methods such as Sanger sequencing or pyrosequencing. Longer read lengths are beneficial for '' de novo'' genome assembly. Ion Torrent semiconductor sequencers produce an average read length of approximately 400 nucleotides per read.

The throughput is currently lower than that of other high-throughput sequencing technologies, although the developers hope to change this by increasing the density of the chip.

Application

The developers of Ion Torrent semiconductor sequencing have marketed it as a rapid, compact and economical sequencer that can be utilized in a large number of laboratories as a bench top machine. The company hopes that their system will take sequencing outside of specialized centers and into the reach of hospitals and smaller laboratories.Pollack, A.
Taking DNA Sequencing to the Masses
in New York Times. 2011: New York. A January 2011 New York Times article

underlines these ambitions.

Due to the ability of alternative sequencing methods to achieve a greater read length (and therefore being more suited to whole genome analysis) this technology may be best suited to small scale applications such as microbial genome sequencing, microbial transcriptome sequencing, targeted sequencing, amplicon sequencing, or for quality testing of sequencing libraries.

References

{{Reflist

External links

Ion Torrent webpage

DNA Electronics Ltd. webpage



Biotechnology
DNA sequencing methods
Molecular biology