EMAGE (e-Mouse Atlas of Gene ExpressionHistorically, EMAGE was an acronym for

Edinburgh Edinburgh is the capital city of Scotland and one of its 32 Council areas of Scotland, council areas. The city is located in southeast Scotland and is bounded to the north by the Firth of Forth and to the south by the Pentland Hills. Edinburgh ...

Mouse Atlas of Gene Expression.) is an online

biological database Biological databases are libraries of biological sciences, collected from scientific experiments, published literature, high-throughput experiment technology, and computational analysis. They contain information from research areas including geno ...

gene expression Gene expression is the process (including its Regulation of gene expression, regulation) by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, ...

data in the developing mouse (''

Mus musculus The house mouse (''Mus musculus'') is a small mammal of the rodent family Muridae, characteristically having a pointed snout, large rounded ears, and a long and almost hairless tail. It is one of the most abundant species of the genus ''Mus (genu ...

'') embryo. The data held in EMAGE is spatially annotated to a framework of 3D mouse embryo models produced b
EMAP
(e-Mouse Atlas Project). These spatial annotations allow users to query EMAGE by spatial pattern as well as by gene name, anatomy term or Gene Ontology (GO) term. EMAGE is a freely available web-based resource funded by the

Medical Research Council (UK) The Medical Research Council (MRC) is responsible for co-coordinating and funding medical research in the United Kingdom. It is part of United Kingdom Research and Innovation (UKRI), which came into operation 1 April 2018, and brings together ...

and based at the

MRC Human Genetics Unit The Medical Research Council (UK) Human Genetics Unit is situated at the Western General Hospital in Edinburgh. It is one of the largest MRC research establishments, housing over two hundred scientists, support staff, research fellows, PhD stude ...

in the Institute of Genetics and Molecular Medicine, Edinburgh, UK.

EMAGE contains ''in situ'' hybridisation,

immunohistochemistry Immunohistochemistry is a form of immunostaining. It involves the process of selectively identifying antigens in cells and tissue, by exploiting the principle of Antibody, antibodies binding specifically to antigens in biological tissues. Alber ...

, and ''in situ'' reporter (e.g. knock-in and

gene trap Gene trapping is a high-throughput approach that is used to introduce insertional mutations across an organism's genome. Method Trapping is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a prom ...

) data. It includes wholemount data,

section Section, Sectioning, or Sectioned may refer to: Arts, entertainment and media * Section (music), a complete, but not independent, musical idea * Section (typography), a subdivision, especially of a chapter, in books and documents ** Section sig ...

data and full 3D OPT ( Optical Projection Tomography) data. The gene expression patterns are mapped into or onto the standard models by a team of

biocurator Biocuration is the field of List of life sciences, life sciences dedicated to organizing biomedical data, information and knowledge into structured formats, such as spreadsheets, Table (information), tables and Resource Description Framework, knowle ...

s, using bespoke mapping software. In addition to the spatial annotations, EMAGE data is also text annotated to provide a text based description of the expression patterns. This text annotation is carried out in collaboration with th
MGI Gene Expression Database (GXD)
using the EMAP mouse anatomy ontology. EMAGE data comes primarily from peer reviewed, published journal articles, and from large scale screens, but also from direct submissions from researches working in the field. Data does not need to be published to be included in EMAGE, however EMAGE is a curated database. Biocurators check the accuracy of the meta-data included in the database entries and as well as performing the spatial annotations of the data. EMAGE entries are designed to adhere to the Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE) specifications, and as such contain information about the submitter/author publication, detection reagent, assay specimen preparation, and experimental procedures as well as the original data images and the spatial and text annotations. EMAGE entries also contain links to a variety of related resources based on the either the gene being assayed, or the assay itself.

References

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External links

EMAGE resource home page
Genetics databases Genetic engineering in the United Kingdom Science and technology in Edinburgh

Contents

See also

References

External links