''Arthrobacter luteus'' (ALU) is a species of
gram-positive bacteria
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall.
Gram-positive bact ...
in the genus ''
Arthrobacter''. ''A. luteus'' is
facultatively anaerobic,
pleomorphic, branching,
non-motile,
non-sporulating, non-
acid-fast
Acid-fastness is a physical property of certain bacterial and eukaryotic cells, as well as some sub-cellular structures, specifically their resistance to decolorization by acids during laboratory staining procedures. Once stained as part of a s ...
,
catalase-positive, and
rod-shaped
A bacillus (), also called a bacilliform bacterium or often just a rod (when the context makes the sense clear), is a rod-shaped bacterium or archaeon. Bacilli are found in many different taxonomic groups of bacteria. However, the name '' Bacil ...
(0.6–1.0 μm × 0.8–10.0 μm).
A
restriction endonuclease
A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class ...
enzyme is extracted from the bacterium and acts at the centre of a
palindromic tetranucleotide sequence to give even-ended
duplex
Duplex (Latin, 'double') may refer to:
Arts and entertainment
* ''Duplex'' (film), or ''Our House'', a 2003 American black comedy film
* Duplex (band), a Dutch electronic music duo
* Duplex (Norwegian duo)
* Duplex!, a Canadian children's music ...
DNA fragments phosphorylated at the
5'-end
Directionality, in molecular bi