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Plating Efficiency
Plating efficiency ("PE") is a measure of the number of colonies originating from single cells. It is a very sensitive test and is often used for determining the nutritional requirements of cells, testing serum lots, measuring the effects of growth factors, and for toxicity testing. Plating Efficiency is the number of cells that grow into colonies per 100 cells inoculated. That is, it is the proportion of cells that attach and grow to the number of cells originally plated, expressed as a percentage. PE can be determined by the following formulae: :\mathrm = \frac \times 100 or :\mathrm = \frac{\#\, \text{cells inoculated \times 100 The first method is more accurate. Cell growth in culture generally undergoes a decline after plating, and graphically, PE is the global minimum (lowest point) of the growth curve at day one, after which growth rises again. The decrease in viable cells after plating is due to "anchorage-dependence"--cells must attach to the bottom of the culture ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Colony (biology)
In biology, a colony is composed of two or more conspecific individuals living in close association with, or connected to, one another. This association is usually for mutual benefit such as stronger defense or the ability to attack bigger prey. Colonies can form in various shapes and ways depending on the organism involved. For instance, the bacterial colony is a cluster of identical cells (clones). These colonies often form and grow on the surface of (or within) a solid medium, usually derived from a single parent cell. Colonies, in the context of development, may be composed of two or more unitary (or solitary) organisms or be modular organisms. Unitary organisms have determinate development (set life stages) from zygote to adult form and individuals or groups of individuals (colonies) are visually distinct. Modular organisms have indeterminate growth forms (life stages not set) through repeated iteration of genetically identical modules (or individuals), and it can be diffi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell (biology)
The cell is the basic structural and functional unit of life forms. Every cell consists of a cytoplasm enclosed within a membrane, and contains many biomolecules such as proteins, DNA and RNA, as well as many small molecules of nutrients and metabolites.Cell Movements and the Shaping of the Vertebrate Body in Chapter 21 of Molecular Biology of the Cell '' fourth edition, edited by Bruce Alberts (2002) published by Garland Science. The Alberts text discusses how the "cellular building blocks" move to shape developing s. It is also common ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Serous Fluid
In physiology, serous fluid or serosal fluid (originating from the Medieval Latin word ''serosus'', from Latin ''serum'') is any of various body fluids resembling serum, that are typically pale yellow or transparent and of a benign nature. The fluid fills the inside of body cavities. Serous fluid originates from serous glands, with secretions enriched with proteins and water. Serous fluid may also originate from mixed glands, which contain both mucous and serous cells. A common trait of serous fluids is their role in assisting digestion, excretion, and respiration. In medical fields, especially cytopathology, serous fluid is a synonym for effusion fluids from various body cavities. Examples of effusion fluid are pleural effusion and pericardial effusion. There are many causes of effusions which include involvement of the cavity by cancer. Cancer in a serous cavity is called a serous carcinoma. Cytopathology evaluation is recommended to evaluate the causes of effusions in these cav ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Growth Factors
A growth factor is a naturally occurring substance capable of stimulating cell proliferation, wound healing, and occasionally cellular differentiation. Usually it is a secreted protein or a steroid hormone. Growth factors are important for regulating a variety of cellular processes. Growth factors typically act as signaling molecules between cells. Examples are cytokines and hormones that bind to specific receptors on the surface of their target cells. They often promote cell differentiation and maturation, which varies between growth factors. For example, epidermal growth factor (EGF) enhances osteogenic differentiation, while fibroblast growth factors and vascular endothelial growth factors stimulate blood vessel differentiation ( angiogenesis). Comparison to cytokines ''Growth factor'' is sometimes used interchangeably among scientists with the term ''cytokine.'' Historically, cytokines were associated with hematopoietic (blood and lymph forming) cells and immun ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Toxicity
Toxicity is the degree to which a chemical substance or a particular mixture of substances can damage an organism. Toxicity can refer to the effect on a whole organism, such as an animal, bacterium, or plant, as well as the effect on a substructure of the organism, such as a cell ( cytotoxicity) or an organ such as the liver (hepatotoxicity). By extension, the word may be metaphorically used to describe toxic effects on larger and more complex groups, such as the family unit or society at large. Sometimes the word is more or less synonymous with poisoning in everyday usage. A central concept of toxicology is that the effects of a toxicant are dose-dependent; even water can lead to water intoxication when taken in too high a dose, whereas for even a very toxic substance such as snake venom there is a dose below which there is no detectable toxic effect. Toxicity is species-specific, making cross-species analysis problematic. Newer paradigms and metrics are evolving to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell Growth
Cell growth refers to an increase in the total mass of a cell, including both cytoplasmic, nuclear and organelle volume. Cell growth occurs when the overall rate of cellular biosynthesis (production of biomolecules or anabolism) is greater than the overall rate of cellular degradation (the destruction of biomolecules via the proteasome, lysosome or autophagy, or catabolism). Cell growth is not to be confused with cell division or the cell cycle, which are distinct processes that can occur alongside cell growth during the process of cell proliferation, where a cell, known as the mother cell, grows and divides to produce two daughter cells. Importantly, cell growth and cell division can also occur independently of one another. During early embryonic development ( cleavage of the zygote to form a morula and blastoderm), cell divisions occur repeatedly without cell growth. Conversely, some cells can grow without cell division or without any progression of the cell cycl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell Culture
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions the need to be kept at body temperature (37 °C) in an incubator. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or rich medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases ( CO2, O2), and regulates the physio-chemical environment ( pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Petri Dish
A Petri dish (alternatively known as a Petri plate or cell-culture dish) is a shallow transparent lidded dish that biologists use to hold growth medium in which cells can be cultured,R. C. Dubey (2014): ''A Textbook Of Biotechnology For Class-XI'', 4th edition, p. 469. originally, cells of bacteria, fungi and small mosses. The container is named after its inventor, German bacteriologist Julius Richard Petri. It is the most common type of culture plate. The Petri dish is one of the most common items in biology laboratories and has entered popular culture. The term is sometimes written in lower case, especially in non-technical literature. What was later called Petri dish was originally developed by German physician Robert Koch in his private laboratory in 1881, as a precursor method. Petri, as assistant to Koch, at Berlin University made the final modifications in 1887 as used today. Penicillin, the first antibiotic, was discovered in 1929 when Alexander Fleming noticed th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Doubling Time
The doubling time is the time it takes for a population to double in size/value. It is applied to population growth, inflation, resource extraction, consumption of goods, compound interest, the volume of malignant tumours, and many other things that tend to grow over time. When the relative growth rate (not the absolute growth rate) is constant, the quantity undergoes exponential growth and has a constant doubling time or period, which can be calculated directly from the growth rate. This time can be calculated by dividing the natural logarithm of 2 by the exponent of growth, or approximated by dividing 70 by the percentage growth rate (more roughly but roundly, dividing 72; see the rule of 72 for details and derivations of this formula). The doubling time is a characteristic unit (a natural unit of scale) for the exponential growth equation, and its converse for exponential decay is the half-life. For example, given Canada's net population growth of 0.9% in the year 2006, d ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Generation Time
In population biology and demography, generation time is the average time between two consecutive generations in the lineages of a population. In human populations, generation time typically ranges from 22 to 33 years. Historians sometimes use this to date events, by converting generations into years to obtain rough estimates of time. Definitions and corresponding formulas The existing definitions of generation time fall into two categories: those that treat generation time as a renewal time of the population, and those that focus on the distance between individuals of one generation and the next. Below are the three most commonly used definitions: The time it takes for the population to grow by a factor of its net reproductive rate The net reproductive rate \textstyle R_0 is the number of offspring an individual is expected to produce during its lifetime (a net reproductive rate of 1 means that the population is at its demographic equilibrium). This definition envisions the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Plenum Press
Springer Science+Business Media, commonly known as Springer, is a German multinational publishing company of books, e-books and peer-reviewed journals in science, humanities, technical and medical (STM) publishing. Originally founded in 1842 in Berlin, it expanded internationally in the 1960s, and through mergers in the 1990s and a sale to venture capitalists it fused with Wolters Kluwer and eventually became part of Springer Nature in 2015. Springer has major offices in Berlin, Heidelberg, Dordrecht, and New York City. History Julius Springer founded Springer-Verlag in Berlin in 1842 and his son Ferdinand Springer grew it from a small firm of 4 employees into Germany's then second largest academic publisher with 65 staff in 1872.Chronology ". Springer Science+Business Media. In 1964, Springer expanded its business internationally, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
New York City
New York, often called New York City or NYC, is the most populous city in the United States. With a 2020 population of 8,804,190 distributed over , New York City is also the most densely populated major city in the United States, and is more than twice as populous as second-place Los Angeles. New York City lies at the southern tip of New York State, and constitutes the geographical and demographic center of both the Northeast megalopolis and the New York metropolitan area, the largest metropolitan area in the world by urban landmass. With over 20.1 million people in its metropolitan statistical area and 23.5 million in its combined statistical area as of 2020, New York is one of the world's most populous megacities, and over 58 million people live within of the city. New York City is a global cultural, financial, entertainment, and media center with a significant influence on commerce, health care and life sciences, research, technology, educa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
