Plating efficiency ("PE") is a measure of the number of
colonies originating from single cells. It is a very sensitive test and is often used for determining the nutritional requirements of
cells
Cell most often refers to:
* Cell (biology), the functional basic unit of life
Cell may also refer to:
Locations
* Monastic cell, a small room, hut, or cave in which a religious recluse lives, alternatively the small precursor of a monastery w ...
, testing
serum
Serum may refer to:
*Serum (blood), plasma from which the clotting proteins have been removed
**Antiserum, blood serum with specific antibodies for passive immunity
* Serous fluid, any clear bodily fluid
* Truth serum, a drug that is likely to mak ...
lots, measuring the effects of
growth factors, and for
toxicity testing.
Plating Efficiency is the number of cells that grow into colonies per 100 cells inoculated. That is, it is the proportion of cells that attach and grow to the number of cells originally plated, expressed as a percentage. PE can be determined by the following formulae:
:
or
:
The first method is more accurate.
Cell growth
Cell growth refers to an increase in the total mass of a cell, including both cytoplasmic, nuclear and organelle volume. Cell growth occurs when the overall rate of cellular biosynthesis (production of biomolecules or anabolism) is greater than ...
in
culture generally undergoes a decline after
plating, and graphically, PE is the global minimum (lowest point) of the growth curve at day one, after which growth rises again. The decrease in viable cells after plating is due to "anchorage-dependence"--cells must attach to the bottom of the culture dish.
Plating Efficiency is one of the parameters typically used to define growth properties of cells in culture. Other common parameters are
doubling time The doubling time is the time it takes for a population to double in size/value. It is applied to population growth, inflation, resource extraction, consumption of goods, compound interest, the volume of malignant tumours, and many other things th ...
("DT") (which is an average of
generation time ("GT")), and saturation density ("SD").
References
*Mather, J.P., and P.E. Roberts, 1998. ''Introduction to Cell and Tissue Culture: Theory and Technique''.
Plenum Press.
New York City and
London.
Plant reproduction