Optical Pooled Screening
Optical pooled screening (OPS) is a type of High-content screening, high-content Single cell#Biology, single-cell genetic screen that profiles the Phenotype, phenotypes of individual Cell (biology), cells by Optical microscope, optical microscopy. The phenotypic profile of each cell is linked to one or several genetic features by Spatial transcriptomics#in situ sequencing, ''in situ'' genotyping. OPS is used to determine the effect of genetic elements on the characteristics of cells and Tissue (biology), tissues. Single-cell screening methods like OPS have been adopted by the biotechnology industry for applications in drug development. High-content pooled single-cell genetic screens became available as a functional genomics technique starting circa 2016. While the genetic intervention (also known as a "genetic perturbation" in CRISPR gene editing#CRISPR screening, CRISPR screening) can be of any type that can be associated with a genetic sequence in the cell, including modifica ...
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