Symbiobacterium Thermophilum
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Symbiobacterium Thermophilum
''Symbiobacterium thermophilum'' is a symbiotic thermophile that depends on co-culture with a ''Bacillus'' strain for growth. It is Gram-negative and tryptophanase-positive, with type strain T(T) (= IAM 14863T). It is the type species of its genus. ''Symbiobacterium'' is related to the Gram-positive Bacillota and Actinomycetota The ''Actinomycetota'' (or ''Actinobacteria'') are a phylum of all gram-positive bacteria. They can be terrestrial or aquatic. They are of great economic importance to humans because agriculture and forests depend on their contributions to soi ..., but belongs to a lineage that is distinct from both.''S. thermophilum'' has a bacillus shaped cell structure with no flagella. This bacterium is located throughout the environment in soils and fertilizers. Cell Structure Although Gram staining ''S. thermophilum'' shows a negative lab result, there are key Gram-negative membrane biosynthesis proteins that it lacks, such as LPS:glycosyltransferase and poly ...
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Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one Cell (biology), biological cell. They constitute a large domain (biology), domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, Hot spring, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the nitrogen fixation, fixation of nitrogen from the Earth's atmosphere, atmosphere. The nutrient cycle includes the decomposition of cadaver, dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulp ...
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Bacillota
The Bacillota (synonym Firmicutes) are a phylum of bacteria, most of which have gram-positive cell wall structure. The renaming of phyla such as Firmicutes in 2021 remains controversial among microbiologists, many of whom continue to use the earlier names of long standing in the literature. The name "Firmicutes" was derived from the Latin words for "tough skin," referring to the thick cell wall typical of bacteria in this phylum. Scientists once classified the Firmicutes to include all gram-positive bacteria, but have recently defined them to be of a core group of related forms called the low- G+C group, in contrast to the Actinomycetota. They have round cells, called cocci (singular coccus), or rod-like forms (bacillus). A few Firmicutes, such as '' Megasphaera'', '' Pectinatus'', ''Selenomonas'' and ''Zymophilus'', have a porous pseudo-outer membrane that causes them to stain gram-negative. Many Bacillota (Firmicutes) produce endospores, which are resistant to desiccation and c ...
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Clostridia
The Clostridia are a highly polyphyletic class of Bacillota, including ''Clostridium'' and other similar genera. They are distinguished from the Bacilli by lacking aerobic respiration. They are obligate anaerobes and oxygen is toxic to them. Species of the class ''Clostridia'' are often but not always Gram-positive (see '' Halanaerobium'') and have the ability to form spores. Studies show they are not a monophyletic group, and their relationships are not entirely certain. Currently, most are placed in a single order called Clostridiales, but this is not a natural group and is likely to be redefined in the future. Most species of the genus ''Clostridium'' are saprophytic organisms that ferment plant polysaccharides and are found in many places in the environment, most notably the soil. However, the genus does contain some human pathogens (outlined below). The toxins produced by certain members of the genus ''Clostridium'' are among the most dangerous known. Examples are tetan ...
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Eubacteriales
The Eubacteriales are an order of bacteria placed within the class Clostridia. Families Eubacteriales comprises the following families: * " Betainaceae" Jones et al. 2019 * " Bianqueaceae" Liu et al. 2021 * " Borkfalkiaceae" Hildebrand, Pallen & Bork 2020 * Caldicoprobacteraceae Yokoyama et al. 2010 * Christensenellaceae Morotomi, Nagai & Watanabe 2012 * Clostridiaceae Pribram 1933 * Defluviitaleaceae Jabari et al. 2012 * Eubacteriaceae Ludwig et al. 2010 * " Feifaniaceae" Liu et al. 2021 * " Galloscillospiraceae" Gilroy et al. 2021 * Gottschalkiaceae Poehlein et al. 2017c * Hungateiclostridiaceae Zhang et al. 2018b * Lachnospiraceae Rainey 2010 * " Mageeibacillaceae" Hildebrand, Pallen & Bork 2020 * " Mogibacteriaceae" Wylensek et al. 2020 * Oscillospiraceae Peshkoff 1940 * Peptoniphilaceae Johnson et al. 2014 * Peptostreptococcaceae Ezaki 2010 * Ruminococcaceae Rainey 2010 * " Pumilibacteraceae" Afrizal et al. 2021 * Thermohalobacteraceae Spring 2021 * Tissierellacea ...
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Thermophile
A thermophile is an organism—a type of extremophile—that thrives at relatively high temperatures, between . Many thermophiles are archaea, though they can be bacteria or fungi. Thermophilic eubacteria are suggested to have been among the earliest bacteria. Thermophiles are found in various geothermally heated regions of the Earth, such as hot springs like those in Yellowstone National Park (see image) and deep sea hydrothermal vents, as well as decaying plant matter, such as peat bogs and compost. Thermophiles can survive at high temperatures, whereas other bacteria or archaea would be damaged and sometimes killed if exposed to the same temperatures. The enzymes in thermophiles function at high temperatures. Some of these enzymes are used in molecular biology, for example the ''Taq'' polymerase used in PCR. "Thermophile" is derived from the el, θερμότητα (''thermotita''), meaning heat, and el, φίλια (''philia''), love. Classification Thermophiles can be ...
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Bacillus
''Bacillus'' (Latin "stick") is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum '' Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-shaped bacteria; and the plural ''Bacilli'' is the name of the class of bacteria to which this genus belongs. ''Bacillus'' species can be either obligate aerobes which are dependent on oxygen, or facultative anaerobes which can survive in the absence of oxygen. Cultured ''Bacillus'' species test positive for the enzyme catalase if oxygen has been used or is present. ''Bacillus'' can reduce themselves to oval endospores and can remain in this dormant state for years. The endospore of one species from Morocco is reported to have survived being heated to 420 °C. Endospore formation is usually triggered by a lack of nutrients: the bacterium divides within its cell wall, and one side then engulfs the other. They are not true spores (i.e., not an offspring). Endospore formatio ...
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Gram-negative
Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. They are characterized by their cell envelopes, which are composed of a thin peptidoglycan cell wall sandwiched between an inner cytoplasmic cell membrane and a bacterial outer membrane. Gram-negative bacteria are found in virtually all environments on Earth that support life. The gram-negative bacteria include the model organism ''Escherichia coli'', as well as many pathogenic bacteria, such as ''Pseudomonas aeruginosa'', '' Chlamydia trachomatis'', and '' Yersinia pestis''. They are a significant medical challenge as their outer membrane protects them from many antibiotics (including penicillin), detergents that would normally damage the inner cell membrane, and lysozyme, an antimicrobial enzyme produced by animals that forms part of the innate immune system. Additionally, the outer leaflet of this membrane comprises a comp ...
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Tryptophanase
The enzyme tryptophanase () catalysis, catalyzes the chemical reaction :L-tryptophan + H2O \rightleftharpoons indole + pyruvate + NH3 This enzyme belongs to the family of lyases, specifically in the "catch-all" class of carbon-carbon lyases. The List of enzymes, systematic name of this enzyme class is L-tryptophan indole-lyase (deaminating; pyruvate-forming). Other names in common use include L-tryptophanase, and L-tryptophan indole-lyase (deaminating). This enzyme participates in tryptophan metabolism and nitrogen metabolism. It has 2 cofactor (biochemistry), cofactors: pyridoxal phosphate, and potassium. Structural studies As of late 2007, 3 tertiary structure, structures have been solved for this class of enzymes, with Protein Data Bank, PDB accession codes 1AX4, 2C44, and 2OQX. Retrieved from Protein Data Bank (PDB) References External links

* EC 4.1.99 Pyridoxal phosphate enzymes Potassium enzymes Enzymes of known structure {{4.1-enzyme-stub ...
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Actinomycetota
The ''Actinomycetota'' (or ''Actinobacteria'') are a phylum of all gram-positive bacteria. They can be terrestrial or aquatic. They are of great economic importance to humans because agriculture and forests depend on their contributions to soil systems. In soil they help to decompose the organic matter of dead organisms so the molecules can be taken up anew by plants. While this role is also played by fungi, ''Actinomycetota'' are much smaller and likely do not occupy the same ecological niche. In this role the colonies often grow extensive mycelia, like a fungus would, and the name of an important order of the phylum, ''Actinomycetales'' (the actinomycetes), reflects that they were long believed to be fungi. Some soil actinomycetota (such as '' Frankia'') live symbiotically with the plants whose roots pervade the soil, fixing nitrogen for the plants in exchange for access to some of the plant's saccharides. Other species, such as many members of the genus ''Mycobacterium ...
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Megabase
A base pair (bp) is a fundamental unit of double-stranded nucleic acids consisting of two nucleobases bound to each other by hydrogen bonds. They form the building blocks of the DNA double helix and contribute to the folded structure of both DNA and RNA. Dictated by specific hydrogen bonding patterns, "Watson–Crick" (or "Watson–Crick–Franklin") base pairs (guanine–cytosine and adenine–thymine) allow the DNA helix to maintain a regular helical structure that is subtly dependent on its nucleotide sequence. The complementary nature of this based-paired structure provides a redundant copy of the genetic information encoded within each strand of DNA. The regular structure and data redundancy provided by the DNA double helix make DNA well suited to the storage of genetic information, while base-pairing between DNA and incoming nucleotides provides the mechanism through which DNA polymerase replicates DNA and RNA polymerase transcribes DNA into RNA. Many DNA-binding prot ...
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