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Sf9 (cells)
Sf9 cells, a Cloning, clonal isolate of ''Spodoptera frugiperda'' Sf21, Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension. Sf9 Rhabdovirus It has previously been shown that some Sf9 cell lines harbor a Negative-strand RNA virus, negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV). However, not all tested Sf9 cells appear to be infected with this virus. SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.{{cite journal, author=Maghodia AB, Jarvis DL, title=Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines. , journal=Virology , year= 2017 , volume= 512 , issue= , pages= 234–245 , pmid=29024851 , doi=10.1016/j.virol.2017.09.025 , pmc=5651996 References External linksCe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
SF9 Cells Phase Contrast Lara Rudman
SF9 (; shortened from Sensational Feeling 9) is a South Korean boy band formed by FNC Entertainment and the company's first dance boy group. The group is composed of eight members: Youngbin, Inseong, Jaeyoon, Dawon, Zuho, Yoo Taeyang, Hwiyoung, and Chani. Originally a nine-member group, Rowoon left the group on September 18, 2023, to focus on acting. They debuted on October 5, 2016, with the release of their first single album, '' Feeling Sensation''. History 2015–2016: NEOZ School and debut The group first performed in Japan on December 11, 2015 with 11 members at that time. In May 2016, the nine member participated as NEOZ Dance on the 2016 reality survival ''d.o.b (Dance or Band)'', competing against other trainees in NEOZ Band (later known as Honeyst) for the chance to debut as a group. NEOZ Dance later won the show with their first song "K.O.", which was later featured in the ''Feeling Sensation'' single album. NEOZ Dance later announced went on to debut as SF9 i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cloning
Cloning is the process of producing individual organisms with identical genomes, either by natural or artificial means. In nature, some organisms produce clones through asexual reproduction; this reproduction of an organism by itself without a mate is known as parthenogenesis. In the field of biotechnology, cloning is the process of creating cloned organisms of Cell (biology), cells and of DNA fragments. The artificial cloning of organisms, sometimes known as reproductive cloning, is often accomplished via somatic-cell nuclear transfer (SCNT), a cloning method in which a viable embryo is created from a somatic cell and an egg cell. In 1996, Dolly (sheep), Dolly the sheep achieved notoriety for being the first mammal cloned from a somatic cell. Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a single living cell is used to clone a large population of cells that contain identical DNA molecules. In bioethics, there are a vari ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spodoptera Frugiperda
The fall armyworm (''Spodoptera frugiperda'') is a species in the order Lepidoptera and one of the species of the fall armyworm moths distinguished by their larval life stage. The term "armyworm" can refer to several species, often describing the large-scale invasive behavior of the species' larval stage. It is regarded as a pest and can damage and destroy a wide variety of crops, which causes large economic damage. Its scientific name derives from ''frugiperda,'' which is Latin for ''lost fruit'', named because of the species' ability to destroy crops. Because of its propensity for destruction, the fall armyworm's habits and possibilities for crop protection have been studied in depth. It is also a notable case for studying sympatric speciation, as it appears to be diverging into two species currently. Another remarkable trait of the larva is that they consistently practice cannibalism, despite its fitness costs. The fall armyworm is active at a different time of year from the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sf21
Sf21 (officially called IPLB-Sf21-AE) is a continuous cell line developed from ovaries of the Fall Army worm, ''Spodoptera frugiperda'', a moth species that is an agricultural pest on corn and other grass species. It was originally developed in the United States at the Henry A. Wallace Beltsville Agricultural Research Center. Sf9 is a substrain ( clone) of these cells that was isolated from Sf21 by researchers at Texas A&M University. Both the clone and parent strains of the cells have been extensively used in research on viruses, especially baculovirus ''Baculoviridae'' is a family of viruses. Arthropods, among the most studied being Lepidoptera, Hymenoptera and Diptera, serve as natural hosts. Currently, 85 species are placed in this family, assigned to four genera. Baculoviruses are known ...es in their use for producing recombinant proteins. References {{cite journal , author=Vaughn, J. L. , author2=Goodwin, R. H. , author3=Tompkins, G. J. , author4=McCawl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Insect Cell Culture
The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from '' Bombyx mori'', ''Mamestra brassicae'', '' Spodoptera frugiperda'', '' Trichoplusia ni'', and ''Drosophila melanogaster ''Drosophila melanogaster'' is a species of fly (an insect of the Order (biology), order Diptera) in the family Drosophilidae. The species is often referred to as the fruit fly or lesser fruit fly, or less commonly the "vinegar fly", "pomace fly" ...'' being of particular interest. Insects cell lines are commonly used in place of prokaryotic ones because post-translational modifications of proteins are possible in insect cells whereas this mechanism is not present in prokaryotic systems. The Sf9 cell line is one of the most commonly used lines in insect cell culture. References and notes Cell culture techniques Biopharma ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Baculovirus
''Baculoviridae'' is a family of viruses. Arthropods, among the most studied being Lepidoptera, Hymenoptera and Diptera, serve as natural hosts. Currently, 85 species are placed in this family, assigned to four genera. Baculoviruses are known to infect insects, with over 600 host species having been described. Immature (larval) forms of lepidopteran species (moths and butterflies) are the most common hosts, but these viruses have also been found infecting sawflies, and mosquitoes. Although baculoviruses are capable of entering mammalian cells in culture, they are not known to be capable of replication in mammalian or other vertebrate animal cells. Starting in the 1940s, they were used and studied widely as biopesticides in crop fields. Baculoviruses contain a circular, double-stranded DNA (dsDNA) genome ranging from 80 to 180 kbp. Historical influence The earliest records of baculoviruses can be found in the literature from as early as the 16th century in reports of "wi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Negative-strand RNA Virus
Negative-strand RNA viruses (−ssRNA viruses) are a group of related viruses that have Sense (molecular biology), negative-sense, single-stranded genomes made of ribonucleic acid (RNA). They have genomes that act as complementary strands from which messenger RNA (mRNA) is synthesized by the viral enzyme RNA-dependent RNA polymerase (RdRp). During replication of the viral genome, RdRp synthesizes a positive-sense antigenome that it uses as a template to create genomic negative-sense RNA. Negative-strand RNA viruses also share a number of other characteristics: most contain a viral envelope that surrounds the capsid, which encases the viral genome, −ssRNA virus genomes are usually linear, and it is common for their genome to be segmented. Negative-strand RNA viruses constitute the phylum ''Negarnaviricota'', in the kingdom ''Orthornavirae'' and realm ''Riboviria''. They are descended from a common ancestor that was a Double-stranded RNA viruses, double-stranded RNA (dsRNA) virus ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rhabdovirus
''Rhabdoviridae'' is a family of negative-strand RNA viruses in the order ''Mononegavirales''. Vertebrates (including mammals and humans), invertebrates, plants, fungi and protozoans serve as natural hosts. Diseases associated with member viruses include rabies encephalitis caused by the rabies virus, and flu-like symptoms in humans caused by vesiculoviruses. The name is derived from Ancient Greek , meaning rod, referring to the shape of the viral particles. The family has 62 genera, most assigned to four subfamilies. Structure The individual virus particles (virions) of rhabdoviruses are composed of RNA, protein, carbohydrate and lipid. They have complex bacilliform or bullet-like shapes. All these viruses have structural similarities and have been classified as a single family. The virions are about 75 nm wide and 180 nm long. Rhabdoviruses are enveloped and have helical nucleocapsids and their genomes are linear, around 11–15 kb in length. Rhabdoviruses ca ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spodoptera Frugiperda Rhabdovirus
''Spodoptera'' is a genus of moths of the family Noctuidae erected by Achille Guenée in 1852. Many are known as pest insects. The larvae are sometimes called armyworms. The roughly thirty species are distributed across six continents. Description No tufts behind collar as in '' Euplexia'', but only tufts present on metathorax. Scales much smoother. Abdominal tufts slight. Fore tibial tufts are very developed. Cilia slightly crenulated. Antennae almost simple. Species * '' Spodoptera abyssinia'' Guenée, 1852 * '' Spodoptera albula'' (Walker, 1857) (orth. var. ''S. albulum'') * '' Spodoptera androgea'' (Stoll, 782 * '' Spodoptera angulata'' (Gaede, 1935) * '' Spodoptera apertura'' (Walker, 1865) * '' Spodoptera cilium'' Guenée, 1852 – grasslawn armyworm * '' Spodoptera compta'' (Walker, 1869) * '' Spodoptera connexa'' (Wileman, 1914) * '' Spodoptera depravata'' (Butler, 1879) * '' Spodoptera dolichos'' (Fabricius, 1794) – sweet potato armyworm * '' Spodoptera eridania'' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Insect Cell Lines
Insects (from Latin ') are Hexapoda, hexapod invertebrates of the class (biology), class Insecta. They are the largest group within the arthropod phylum. Insects have a chitinous exoskeleton, a three-part body (Insect morphology#Head, head, Thorax (insect anatomy), thorax and abdomen (insect anatomy), abdomen), three pairs of jointed Arthropod leg, legs, compound eyes, and a pair of antenna (biology), antennae. Insects are the most diverse group of animals, with more than a million described species; they represent more than half of all animal species. The insect nervous system consists of a insect brain, brain and a ventral nerve cord. Most insects reproduce Oviparous, by laying eggs. Insects Respiratory system of insects, breathe air through a system of Spiracle (arthropods), paired openings along their sides, connected to Trachea#Invertebrates, small tubes that take air directly to the tissues. The blood therefore does not carry oxygen; it is only partly contained in ves ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
