Microcrystallization
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Microcrystallization
Microcrystallization (or microcrystal test) is a method for identifying lichen product, lichen metabolites that was predominantly used before the advent of more advanced techniques such as thin-layer chromatography and high-performance liquid chromatography. Developed primarily by Yasuhiko Asahina, this approach relies on the formation of distinctive crystals from lichen extracts. Although now superseded by modern analytical chemistry, analytical methods, microcrystallization still holds importance for compound purification and analysis using X-ray crystallography. History Between 1936 and 1940, Japanese chemist and lichenologist Yasuhiko Asahina published a series of papers in the ''Journal of Japanese Botany'' detailing the microcrystallization technique. This simple and rapid method allowed for the identification of major metabolites in hundreds of lichen species, contributing significantly to taxonomy (biology), taxonomic research. The technique was introduced to western liche ...
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Norstictic Acid Crystals
Norstictic acid is a depsidone produced as a secondary metabolites in lichens. The compound contains both an aldehyde carbonyl group and an adjacent hydroxyl group in its molecular structure, which enables it to form chelation, complexes with certain metals. This property was demonstrated in studies of lichens growing on copper-rich substrates, where norstictic acid forms complexes with copper ions in the (outer layer) of the lichen thallus, resulting in a distinctive green-yellow coloration. The formation of these copper-norstictic acid complexes appears to be selective, as evidenced by comparing it to the structurally similar stictic acid, which lacks the adjacent hydroxy group and does not form copper complexes. This metal-binding capability may have implications for both lichen taxonomy and the use of lichens as biological indicators of metal mineralization, though the formation of such complexes is not the only mechanism by which lichens can tolerate high metal concentrations i ...
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Filtration
Filtration is a physical separation process that separates solid matter and fluid from a mixture using a ''filter medium'' that has a complex structure through which only the fluid can pass. Solid particles that cannot pass through the filter medium are described as ''oversize'' and the fluid that passes through is called the ''filtrate''. Oversize particles may form a filter cake on top of the filter and may also block the filter lattice, preventing the fluid phase from crossing the filter, known as ''blinding''. The size of the largest particles that can successfully pass through a filter is called the effective ''pore size'' of that filter. The separation of solid and fluid is imperfect; solids will be contaminated with some fluid and filtrate will contain fine particles (depending on the pore size, filter thickness and biological activity). Filtration occurs both in nature and in engineered systems; there are biological, geological, and industrial forms. In everyday us ...
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Spirit-lamp
A portable stove is a cooking stove specially designed to be portable and lightweight, used in camping, picnicking, backpacking, or other use in remote locations where an easily transportable means of cooking or heating is needed. Portable stoves can be used in diverse situations, such as for outdoor food service and catering and in field hospitals. Since the invention of the portable stove in the 19th century, a wide variety of designs and models have seen use in a number of different applications. Portable stoves can be broken down into several broad categories based on the type of fuel used and stove design: unpressurized stoves that use solid or liquid fuel placed in the burner before ignition; stoves that use a volatile liquid fuel in a pressurized burner; bottled gas stoves; and gravity-fed "spirit" stoves. History Early examples The shichirin, a lightweight charcoal stove, has been used in Japan in much the same form since at least the Edo period (1603-1868). Old shich ...
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Pipette
A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a Vacuum, partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid. Measurement accuracy varies greatly depending on the instrument. History The first simple pipettes were made of glass, such as Pasteur pipettes. Large pipettes continue to be made of glass; others are made of squeezable plastic for situations where an exact volume is not required. During or prior to 1877, Joseph Lister (1827 – 1912) invented the first adjustable micropipette, consisting of a "pipette with a syringe" (photograph shown at right). The micr ...
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Test Tube
A test tube, also known as a culture tube or sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom. Test tubes are usually placed in special-purpose test tube rack, racks. Types and usage Chemistry Test tubes intended for general chemical work are usually made of glass, for its relative resistance to heat. Tubes made from expansion-resistant glasses, mostly borosilicate glass or fused quartz, can withstand high temperatures up to several hundred degrees Celsius. Chemistry tubes are available in a multitude of lengths and widths, typically from 10 to 20 mm wide and 50 to 200 mm long. The top often features a flared lip to aid pouring out the contents. A chemistry test tube typically has a flat bottom, a round bottom, or a conical bottom. Some test tubes are made to accept a ground glass joint, ground glass stopper or a screw cap. They are often provided w ...
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Microscope
A microscope () is a laboratory equipment, laboratory instrument used to examine objects that are too small to be seen by the naked eye. Microscopy is the science of investigating small objects and structures using a microscope. Microscopic means being invisible to the eye unless aided by a microscope. There are many types of microscopes, and they may be grouped in different ways. One way is to describe the method an instrument uses to interact with a sample and produce images, either by sending a beam of light or electrons through a sample in its optical path, by detecting fluorescence, photon emissions from a sample, or by scanning across and a short distance from the surface of a sample using a probe. The most common microscope (and the first to be invented) is the optical microscope, which uses lenses to refract visible light that passed through a microtome, thinly sectioned sample to produce an observable image. Other major types of microscopes are the fluorescence micro ...
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Polarized Light
, or , is a property of transverse waves which specifies the geometrical orientation of the oscillations. In a transverse wave, the direction of the oscillation is perpendicular to the direction of motion of the wave. One example of a polarized transverse wave is vibrations traveling along a taut string, for example, in a musical instrument like a guitar string. Depending on how the string is plucked, the vibrations can be in a vertical direction, horizontal direction, or at any angle perpendicular to the string. In contrast, in longitudinal waves, such as sound waves in a liquid or gas, the displacement of the particles in the oscillation is always in the direction of propagation, so these waves do not exhibit polarization. Transverse waves that exhibit polarization include electromagnetic waves such as light and radio waves, gravitational waves, and transverse sound waves ( shear waves) in solids. An electromagnetic wave such as light consists of a coupled oscillating elec ...
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Acetic Acid
Acetic acid , systematically named ethanoic acid , is an acidic, colourless liquid and organic compound with the chemical formula (also written as , , or ). Vinegar is at least 4% acetic acid by volume, making acetic acid the main component of vinegar apart from water. Historically, vinegar was produced from the third century BC and was likely the first acid to be produced in large quantities. Acetic acid is the second simplest carboxylic acid (after formic acid). It is an important Reagent, chemical reagent and industrial chemical across various fields, used primarily in the production of cellulose acetate for photographic film, polyvinyl acetate for wood Adhesive, glue, and synthetic fibres and fabrics. In households, diluted acetic acid is often used in descaling agents. In the food industry, acetic acid is controlled by the E number, food additive code E260 as an acidity regulator and as a condiment. In biochemistry, the acetyl group, derived from acetic acid, is funda ...
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Ethanol
Ethanol (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound with the chemical formula . It is an Alcohol (chemistry), alcohol, with its formula also written as , or EtOH, where Et is the pseudoelement symbol for ethyl group, ethyl. Ethanol is a Volatility (chemistry), volatile, flammable, colorless liquid with a characteristic wine-like odor and pungent taste. As a psychoactive depressant, it is the active ingredient in alcoholic beverages, and the second most consumed drug globally behind caffeine. Ethanol is naturally produced by the fermentation process of sugars by yeasts or via petrochemical processes such as ethylene hydration. Historically it was used as a general anesthetic, and has modern medical applications as an antiseptic, disinfectant, solvent for some medications, and antidote for methanol poisoning and ethylene glycol poisoning. It is used as a chemical solvent and in the Chemical synthesis, synthesis of orga ...
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Glycerol
Glycerol () is a simple triol compound. It is a colorless, odorless, sweet-tasting, viscous liquid. The glycerol backbone is found in lipids known as glycerides. It is also widely used as a sweetener in the food industry and as a humectant in pharmaceutical formulations. Because of its three hydroxyl groups, glycerol is miscible with water and is Hygroscopy, hygroscopic in nature. Modern use of the word glycerine (alternatively spelled glycerin) refers to commercial preparations of less than 100% purity, typically 95% glycerol. Structure Although chirality, achiral, glycerol is prochirality, prochiral with respect to reactions of one of the two primary alcohols. Thus, in substituted derivatives, the Glycerophospholipid#Nomenclature and stereochemistry, stereospecific numbering labels the molecule with a ''sn''- prefix before the stem name of the molecule. Production Natural sources Glycerol is generally obtained from plant and animal sources where it occurs in triglycerides, est ...
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Reagent
In chemistry, a reagent ( ) or analytical reagent is a substance or compound added to a system to cause a chemical reaction, or test if one occurs. The terms ''reactant'' and ''reagent'' are often used interchangeably, but reactant specifies a substance ''consumed'' in the course of a chemical reaction. ''Solvents'', though involved in the reaction mechanism, are usually not called reactants. Similarly, ''catalysts'' are not consumed by the reaction, so they are not reactants. In biochemistry, especially in connection with enzyme-catalyzed reactions, the reactants are commonly called substrates. Definitions Organic chemistry In organic chemistry, the term "reagent" denotes a chemical ingredient (a compound or mixture, typically of inorganic or small organic molecules) introduced to cause the desired transformation of an organic substance. Examples include the Collins reagent, Fenton's reagent, and Grignard reagents. Analytical chemistry In analytical chemistry, a reag ...
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Cover Glass
Cover or covers may refer to: Packaging * Another name for a lid * Cover (philately), generic term for envelope or package * Album cover, the front of the packaging * Book cover or magazine cover ** Book design ** Back cover copy, part of copywriting * CD and DVD cover, CD and DVD packaging * Smartphone cover, a mobile phone accessory that protects a mobile phone People * Cover (surname) Arts, entertainment, and media Music Albums ;Cover * ''Cover'' (Tom Verlaine album), 1984 * ''Cover'' (Joan as Policewoman album), 2009 ;Covered * ''Covered'' (Cold Chisel album), 2011 * ''Covered'' (Macy Gray album), 2012 * ''Covered'' (Robert Glasper album), 2015 ;Covers * ''Covers'' (Beni album), 2012 * ''Covers'' (Regine Velasquez album), 2004 * ''Covers'' (Placebo album), 2003 * ''Covers'' (Show of Hands album), 2000 * ''Covers'' (James Taylor album), 2008 * ''Covers'' (Fayray album), 2005 * ''Covers'' (Deftones album), 2011 * ''Covers'' (The Smithereens album), ...
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