Il Vento E Le Rose
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Il Vento E Le Rose
Il Vento e le Rose ( ja, 愛するということ Ai-suru to iu koto; en, The Awakening of Love) is a 2009 Japanese-Italian movie directed by Elisa Bolognini. Set in Italy, it tells the story of an inhibited young woman from a small Italian town who discovers passion and sexuality when she meets a free-spirited and glamorous Japanese woman. Cast and characters *Kano sisters, Kanō Kyōko as Koko *Maria Cocchiarella Arismendi as Giorgia *Raffaella Panichi as Giorgia's grandmother *Antonio Matessich as gentleman *Alessandro Calabrò as Andrea *Vincent Papa (actor), Vincent Papa as Bulter *Alessio Sica as Black-haired man *Paride Moccia as The blonde *Romano Fortuna as Servant See also * List of LGBT-related films directed by women References External links * (in Japanese) (archive) ''The Awakening of Love''at Pony Canyon * ''The Awakening of Love''
at Japanese Film Database 2009 films 2009 LGBT-related films 2009 romantic drama films 2000s Italian-language films Japa ...
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Elisa Bolognini
The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a medical diagnosis, diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme and then any unbound antibodies are removed. In the final step, a substance containing the enzyme's Enzyme substrate, substrate is added. If there was binding, the subsequent reaction produces a detectable signal, most commonly a color change. Pe ...
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