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Dieterle Stain
The Dieterle stain is a way of marking tissue for microscopic examination. The key reagent of Dieterle stain is silver nitrate. It can stain microbes like ''Treponema pallidum'' in grey or black and background in yellow. It is used to find the organisms that cause cat-scratch disease ''(Bartonella henselae'') and syphilis (''Treponema pallidum'') and sensitive for ''Mycobacterium tuberculosis''. Additional images Image:Treponema pallidum - very high mag.jpg See also *Staining Staining is a technique used to enhance contrast in samples, generally at the Microscope, microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissue (biology), tissues), in cytology (microscopic ... * Warthin–Starry stain References External linksDieterle stain{{Webarchive, url=https://web.archive.org/web/20160303194222/http://www.mondofacto.com/facts/dictionary?Dieterle%27s+stain , date=2016-03-03 - mondofacto.com. Staining ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Treponema Pallidum - Very High Mag - Extreme Crop
''Treponema'' is a genus of spiral-shaped bacteria. The major treponeme species of human pathogens is ''Treponema pallidum'', whose subspecies are responsible for diseases such as syphilis, bejel, and yaws. ''Treponema carateum'' is the cause of pinta. ''Treponema paraluiscuniculi'' is associated with syphilis in rabbits. ''Treponema succinifaciens'' has been found in the gut microbiome of traditional rural human populations. __TOC__ Phylogeny The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI). Unassigned ''Treponema'' species: * "''Ca.'' T. caballi" Gilroy et al. 2022 * ''T. calligyrum'' Noguchi 1913 * '' T. carateum'' Brumpt 1939 ( pinta-causing ''Treponema'') * "''Ca.'' T. equi" Gilroy et al. 2022 non (Novy & Napp 1906) Noguchi 1928 * "''Ca.'' T. equifaecale" Gilroy et al. 2022 * "''Ca.'' T. intracellulare" Ohkuma et al. 2015 corrig. Oren et al. 2020 * "'' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Silver Nitrate
Silver nitrate is an inorganic compound with chemical formula . It is a versatile precursor to many other silver compounds, such as those used in photography. It is far less sensitive to light than the halides. It was once called ''lunar caustic'' because silver was called ''luna'' by ancient alchemists who associated silver with the moon. In solid silver nitrate, the silver ions are three- coordinated in a trigonal planar arrangement. Synthesis and structure Albertus Magnus, in the 13th century, documented the ability of nitric acid to separate gold and silver by dissolving the silver. Indeed silver nitrate can be prepared by dissolving silver in nitric acid followed by evaporation of the solution. The stoichiometry of the reaction depends upon the concentration of nitric acid used. :3 Ag + 4 HNO3 (cold and diluted) → 3 AgNO3 + 2 H2O + NO :Ag + 2 HNO3 (hot and concentrated) → AgNO3 + H2O + NO2 The structure of silver nitrate has been examined by X-ray crystallography se ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cat-scratch Disease
Cat-scratch disease (CSD) is an infectious disease that most often results from a scratch or bite of a cat. Symptoms typically include a non-painful bump or blister at the site of injury and painful and swollen lymph nodes. People may feel tired, have a headache, or a fever. Symptoms typically begin within 3–14 days following infection. Cat-scratch disease is caused by the bacterium '' Bartonella henselae'' which is believed to be spread by the cat's saliva. Young cats pose a greater risk than older cats. Occasionally dog scratches or bites may be involved. Diagnosis is generally based on symptoms. Confirmation is possible by blood tests. The primary treatment is supportive. Antibiotics speed healing and are recommended in those with severe disease or immune problems. Recovery typically occurs within 4 months but can require a year. About 1 in 10,000 people are affected. It is more common in children. Signs and symptoms Cat-scratch disease commonly presents as tender, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Syphilis
Syphilis () is a sexually transmitted infection caused by the bacterium ''Treponema pallidum'' subspecies ''pallidum''. The signs and symptoms depend on the stage it presents: primary, secondary, latent syphilis, latent or tertiary. The primary stage classically presents with a single chancre (a firm, painless, non-itchy Ulcer_(dermatology), skin ulceration usually between 1 cm and 2 cm in diameter), though there may be multiple sores. In secondary syphilis, a diffuse rash occurs, which frequently involves the palms of the hands and soles of the feet. There may also be sores in the mouth or vagina. Latent syphilis has no symptoms and can last years. In tertiary syphilis, there are Gumma (pathology), gummas (soft, non-cancerous growths), neurological problems, or heart symptoms. Syphilis has been known as "The Great Imitator, the great imitator", because it may cause symptoms similar to many other diseases. Syphilis is most commonly spread through human sexual activi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sensitivity (test)
In medicine and statistics, sensitivity and specificity mathematically describe the accuracy of a test that reports the presence or absence of a medical condition. If individuals who have the condition are considered "positive" and those who do not are considered "negative", then sensitivity is a measure of how well a test can identify true positives and specificity is a measure of how well a test can identify true negatives: * Sensitivity (true positive rate) is the probability of a positive test result, conditioned on the individual truly being positive. * Specificity (true negative rate) is the probability of a negative test result, conditioned on the individual truly being negative. If the true status of the condition cannot be known, sensitivity and specificity can be defined relative to a " gold standard test" which is assumed correct. For all testing, both diagnoses and screening, there is usually a trade-off between sensitivity and specificity, such that higher sensiti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mycobacterium Tuberculosis
''Mycobacterium tuberculosis'' (M. tb), also known as Koch's bacillus, is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, ''M. tuberculosis'' has an unusual, waxy coating on its cell surface primarily due to the presence of mycolic acid. This coating makes the cells impervious to Gram staining, and as a result, ''M. tuberculosis'' can appear weakly Gram-positive. Acid-fastness, Acid-fast stains such as Ziehl–Neelsen stain, Ziehl–Neelsen, or Fluorescence, fluorescent stains such as Auramine O, auramine are used instead to identify ''M. tuberculosis'' with a microscope. The physiology of ''M. tuberculosis'' is highly aerobic organism, aerobic and requires high levels of oxygen. Primarily a pathogen of the mammalian respiratory system, it infects the lungs. The most frequently used diagnostic methods for tuberculosis are the Mantoux test, tuberculin skin test, Acid-Fast Stain, aci ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Staining
Staining is a technique used to enhance contrast in samples, generally at the Microscope, microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissue (biology), tissues), in cytology (microscopic study of cell (biology), cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell (biology), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Warthin–Starry Stain
The Warthin–Starry stain (WS) is a silver nitrate-based staining method (a silver stain) used in histology. It was first introduced in 1920 by American pathologists Aldred Scott Warthin (1866–1931) and Allen Chronister Starry (1890–1973), for the detection of spirochetes. It has been considered a standard stain for the detection of spirochetes, and is also used to stain ''Helicobacter pylori'', ''Lawsonia intracellularis'', ''Microsporidia'', and particulates. It is also important for confirmation of ''Bartonella henselae'', a causative organism in cat-scratch disease. Warthin–Starry stains organisms dark brown to black, and the background light golden brown/golden yellow. See also *Dieterle stain The Dieterle stain is a way of marking tissue for microscopic examination. The key reagent of Dieterle stain is silver nitrate. It can stain microbes like ''Treponema pallidum'' in grey or black and background in yellow. It is used to find the org ... References External l ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
