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Competition-ChIP
Competition-ChIP is variant of the Chip-Sequencing ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated prot ... protocol, used to measure relative binding dynamics of a transcription factor (TF) on DNA. Since TF occupancy measures are thought to be a poor predictor of TF function at a given locus, Competition-ChIP is much more strongly linked to function than occupancy. The technique was originally developed in Jason D. Lieb's Lab in 2011. References {{Reflist Protein methods Molecular biology techniques ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chip-Sequencing
ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest. Previously, ChIP-on-chip was the most common technique utilized to study these protein–DNA relations. Uses ChIP-seq is primarily used to determine how transcription factors and other chromatin-associated proteins influence phenotype-affecting mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states. This epigenetic information is complementary to genotype and expression analysis. ChIP-seq technology is currently seen primarily as an alternative to ChIP-chip which requires a hybridization array. This introduces some bias, as an array is restri ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Jason D
Jason ( ; ) was an ancient Greek mythological hero and leader of the Argonauts, whose quest for the Golden Fleece featured in Greek literature. He was the son of Aeson, the rightful king of Iolcos. He was married to the sorceress Medea. He was also the great-grandson of the messenger god Hermes, through his mother's side. Jason appeared in various literary works in the classical world of Greece and Rome, including the epic poem ''Argonautica'' and the tragedy ''Medea''. In the modern world, Jason has emerged as a character in various adaptations of his myths, such as the 1963 film '' Jason and the Argonauts'' and the 2000 TV miniseries of the same name. Persecution by Pelias Pelias (Aeson's half-brother) was power-hungry and sought to gain dominion over all of Thessaly. Pelias was the progeny of a union between their shared mother, Tyro ("high born Tyro"), the daughter of Salmoneus, and the sea god Poseidon. In a bitter feud, he overthrew Aeson (the rightful king), kill ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Protein Methods
Protein methods are the techniques used to study proteins. There are experimental methods for studying proteins (e.g., for detecting proteins, for isolating and purifying proteins, and for characterizing the structure and function of proteins, often requiring that the protein first be purified). Computational methods typically use computer programs to analyze proteins. However, many experimental methods (e.g., mass spectrometry) require computational analysis of the raw data. Genetic methods Experimental analysis of proteins typically requires expression and purification of proteins. Expression is achieved by manipulating DNA that encodes the protein(s) of interest. Hence, protein analysis usually requires DNA methods, especially cloning. Some examples of genetic methods include conceptual translation, Site-directed mutagenesis, using a fusion protein, and matching allele with disease states. Some proteins have never been directly sequenced, however by translating codons from kno ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
