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Centrifugal Partition Chromatography
Centrifugal partition chromatography is a special chromatographic technique where both stationary and mobile phase are liquid, and the stationary phase is immobilized by a strong centrifugal force. Centrifugal partition chromatography consists of a series-connected network of extraction cells, which operates as elemental extractors, and the efficiency is guaranteed by the cascade. History In the 1940s Craig invented the first apparatus to conduct countercurrent partitioning; he called this the countercurrent distribution Craig apparatus. The apparatus consists of a series of glass tubes that are designed and arranged such that the lighter liquid phase is transferred from one tube to the next. The next major milestone was droplet countercurrent chromatography (DCCC). It uses only gravity to move the mobile phase through the stationary phase which is held in long vertical tubes connected in series. The modern era of CCC began with the development of the planetary centrifuge by Ito w ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromatography
In chemical analysis, chromatography is a laboratory technique for the Separation process, separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the ''stationary phase'' is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation. Chromatography may be ''preparative'' or ''analytical' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Stokes' Law
In fluid dynamics, Stokes' law gives the frictional force – also called drag force – exerted on spherical objects moving at very small Reynolds numbers in a viscous fluid. It was derived by George Gabriel Stokes in 1851 by solving the Stokes flow limit for small Reynolds numbers of the Navier–Stokes equations.Batchelor (1967), p. 233. Statement of the law The force of viscosity on a small sphere moving through a viscous fluid is given by: :_ = - 6 \pi \mu R where (in SI units): * _ is the frictional force – known as Stokes' drag – acting on the interface between the fluid and the particle (newtons, kg m s−2); * (some authors use the symbol ) is the dynamic viscosity ( Pascal-seconds, kg m−1 s−1); * is the radius of the spherical object (meters); * is the flow velocity relative to the object (meters per second). Note the minus sign in the equation, the drag force points in the opposite direction to the relative velocity: drag opposes the motion. Stokes' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Surface Tension
Surface tension is the tendency of liquid surfaces at rest to shrink into the minimum surface area possible. Surface tension (physics), tension is what allows objects with a higher density than water such as razor blades and insects (e.g. Gerridae, water striders) to float on a water surface without becoming even partly submerged. At liquid–air interfaces, surface tension results from the greater attraction of liquid molecules to each other (due to Cohesion (chemistry), cohesion) than to the molecules in the air (due to adhesion). There are two primary mechanisms in play. One is an inward force on the surface molecules causing the liquid to contract. Second is a tangential force parallel to the surface of the liquid. This ''tangential'' force is generally referred to as the surface tension. The net effect is the liquid behaves as if its surface were covered with a stretched elastic membrane. But this analogy must not be taken too far as the tension in an elastic membrane i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Countercurrent Chromatography
Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid Stationary phase (chemistry), stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force and is used to separate, identify, and quantify the chemical components of a mixture. In its broadest sense, countercurrent chromatography encompasses a collection of related liquid chromatography techniques that employ two Miscibility, immiscible liquid phases without a solid support. The two liquid phases come in contact with each other as at least one phase is pumped through a Chromatography column, column, a hollow tube or a series of chambers connected with channels, which contains both phases. The resulting dynamic mixing and settling action allows the components to be separated by their respective solubilities in the two phases. A wide variety of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laboratory
A laboratory (; ; colloquially lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratories are found in a variety of settings such as schools, universities, privately owned research institutions, corporate research and testing facilities, government regulatory and forensic investigation centers, physicians' offices, clinics, hospitals, regional and national referral centers, and even occasionally personal residences. Overview The organisation and contents of laboratories are determined by the differing requirements of the specialists working within. A physics laboratory might contain a particle accelerator or vacuum chamber, while a metallurgy laboratory could have apparatus for casting or refining metals or for testing their strength. A chemist or biologist might use a wet laboratory, while a psychologist's laboratory might be a room with one-way mirrors and hidden ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Radial Chromatography
Radial chromatography is a form of chromatography, a preparatory technique for separating chemical mixtures. It can also be referred to as centrifugal thin-layer chromatography. It is a common technique for isolating compounds and can be compared to column chromatography as a similar process. A common device used for this technique is a Chromatotron. Here the solvent travels from the center of the circular chromatography silica layered on a plate towards the periphery. The entire system is kept covered in order to prevent evaporation of solvent while developing a chromatogram In chemical analysis, chromatography is a laboratory technique for the Separation process, separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it .... The wick at the center of system drips solvent into the system which the provides the mobile phase and moves the sample radially to form the sample spots of d ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
