|
CRISPR
CRISPR (; acronym of clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. Each sequence within an individual prokaryotic CRISPR is derived from a DNA fragment of a bacteriophage that had previously infected the prokaryote or one of its ancestors. These sequences are used to detect and destroy DNA from similar bacteriophages during subsequent infections. Hence these sequences play a key role in the antiviral (i.e. anti-phage) defense system of prokaryotes and provide a form of heritable, acquired immunity. CRISPR is found in approximately 50% of sequenced bacterial genomes and nearly 90% of sequenced archaea. Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology kno ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Crispr
CRISPR (; acronym of clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. Each sequence within an individual prokaryotic CRISPR is derived from a DNA fragment of a bacteriophage that had previously infected the prokaryote or one of its ancestors. These sequences are used to detect and destroy DNA from similar bacteriophages during subsequent infections. Hence these sequences play a key role in the antiviral (i.e. anti- phage) defense system of prokaryotes and provide a form of heritable, acquired immunity. CRISPR is found in approximately 50% of sequenced bacterial genomes and nearly 90% of sequenced archaea. Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
The New York Times
''The New York Times'' (''NYT'') is an American daily newspaper based in New York City. ''The New York Times'' covers domestic, national, and international news, and publishes opinion pieces, investigative reports, and reviews. As one of the longest-running newspapers in the United States, the ''Times'' serves as one of the country's Newspaper of record, newspapers of record. , ''The New York Times'' had 9.13 million total and 8.83 million online subscribers, both by significant margins the List of newspapers in the United States, highest numbers for any newspaper in the United States; the total also included 296,330 print subscribers, making the ''Times'' the second-largest newspaper by print circulation in the United States, following ''The Wall Street Journal'', also based in New York City. ''The New York Times'' is published by the New York Times Company; since 1896, the company has been chaired by the Ochs-Sulzberger family, whose current chairman and the paper's publ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Archaeoglobus Fulgidus
''Archaeoglobus'' is a genus of archaeans in the phylum Euryarchaeota. ''Archaeoglobus'' can be found in high-temperature oil fields where they may contribute to oil field souring. Metabolism ''Archaeoglobus'' grow anaerobically at extremely high temperatures between 60 and 95 °C, with optimal growth at 83 °C (ssp. ''A. fulgidus'' VC-16). They are sulfate-reducing archaea, coupling the reduction of sulfate to sulfide with the oxidation of many different organic carbon sources, including complex polymers. ''A. lithotrophicus'' live chemolitho-autotrophically from hydrogen, sulfate and carbon dioxide. Also ''A. profundus'' grow lithotrophically, but while this species needs acetate and CO2 for biosynthesis they are heterotroph. The complete ''A. fulgidus'' genome sequence revealed the presence of a nearly complete set of genes for methanogenesis. The function of these genes in ''A. fulgidus'' remains unknown, while the lack of the enzyme methyl-CoM reductase does ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ruud Jansen
Ruud and Rud are surnames of Norwegian origin. Both are also Norwegian place names of numerous farmsteads named Rud or Ruud from Old Norse ''ruð'' meaning clearing. Ruud is also a Dutch masculine given name meaning "famous wolf" although it is also often short for Rudolf. Ruud * Asbjørn Ruud (1919–1986), Norwegian ski jumper * Barrett Ruud (born 1983), American football linebacker * Birger Ruud (1911–1998), Norwegian ski jumper * Bo Ruud (born 1984), American football linebacker * Casper Ruud (born 1998), Norwegian tennis player * Christian Ruud (born 1972), Norwegian tennis player * David Ruud (born 1980), Swedish motorcycle speedway rider * Edwin Ruud (1854–1932), Norwegian-American mechanical engineer and inventor * Espen Ruud (born 1984), Norwegian football defender * Joseph Ruud (born 1981), American professional wrestler better known as (Erick) Rowan * Maria Ruud (born 1960), American politician and a member of the Minnesota House of Representatives * Mi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Haloferax Volcanii
''Haloferax volcanii'' is a species of archaean in the genus '' Haloferax'' in the Archaea. Description and significance ''Haloferax volcanii'' is a halophilic mesophile archaeon that can be isolated from hypersaline environments such as: the Dead Sea, the Great Salt Lake, and oceanic environments with high sodium chloride concentrates. ''Haloferax volcanii'' is noteworthy because it can be cultured without much difficulty, rare for an extremophile. ''H. volcanii'' is chemoorganotrophic, metabolizing sugars as a carbon source. It is primarily aerobic, but is capable of anaerobic respiration under anoxic conditions. Recently an isolate of this species was studied by researchers at University of California, Berkeley, as part of a project on the survival of haloarchaea on Mars. Genome structure The genome of ''H. volcanii'' consists of a large (4 Mb), multicopy chromosome and several megaplasmids. The complete genome of the wild-type strain of ''H. volcanii'' (DS2) consists ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Haloarcula
''Haloarcula'' (common abbreviation ''Har.'') is a genus of extreme halophilic Archaeon, Archaea in the class of Halobacteria. Cell structure ''Haloarcula'' species can be distinguished from other genera in the family Halobacteriaceae by the presence of specific derivatives of TGD-2 polar lipids. ''H. quadrata'' has predominantly flat, square-shaped, somewhat pleomorphic cells. Metabolism ''Haloarcula quadrata'' was first isolated when researchers were attempting to culture ''Haloquadratum walsbyi'', a haloarchaeon that was thought to be unculturable until 2004. Similar to other halophilic archaea, ''Haloarcula'' species grow optimally at 40–45 °C. Growth appears in sheets of up to 65 cells often in the shape of a square or triangle. Taxonomy The genus of ''Haloarcula'' was long grouped with other halophilic archaea such as ''Halobacterium'' until genomic analysis prompted to reorder this genus in the new family of Haloarculaceae. Phylogeny The currently accepted taxono ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Haloferax
''Haloferax'' (common abbreviation: ''Hfx.'') is a genus of halobacteria in the order Haloferacaceae. Genetic exchange Cells of ''H. mediterranei'' and cells of the related species '' H. volcanii'' can undergo a process of genetic exchange between two cells which involves cell fusion resulting in a heterodiploid cell (containing two different chromosomes in one cell). Although this genetic exchange ordinarily occurs between two cells of the same species, it can also occur at a lower frequency between an ''H. mediterranei'' and an ''H. volcani'' cell. These two species have an average nucleotide sequence identity of 86.6%. During this exchange process, a diploid cell is formed that contains the full genetic repertoire of both parental cells, and genetic recombination is facilitated. Subsequently, the cells separate, giving rise to recombinant cells. Taxonomy As of 2022, 13 species are validly published under the genus ''Haloferax''. ;Proposed species Several species and novel ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
University Of Alicante
The University of Alicante (, ; , ; also known by the acronym ''UA'') was established in 1979 on the basis of the Center for University Studies (CEU), which was founded in 1968. The university main campus is located in San Vicente del Raspeig/Sant Vicent del Raspeig, bordering the city of Alicante to the north. As of 2011/12 academic year, there are approximately 27,500 students studying there. History The university inherits the legacy of the University of Orihuela that was established by Papal Bull in 1545 and remained open for two centuries (1610–1808). Studies The University of Alicante offers courses in more than fifty degrees. It comprises over seventy departments and research groups in areas of Social Science and Law, Experimental science, Technology, Liberal Arts, Education and Health Sciences, and five research institutes. Almost all classes are taught in Spanish language, some are in English, in particular, in computer science and in business degrees, and a few ar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Francisco Mojica
Francisco Juan Martínez Mojica (born 5 October 1963) is a Spanish molecular biologist and microbiologist at the University of Alicante in Spain. He is known for his discovery of repetitive, functional DNA sequences in bacteria which he named CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). These were later developed into the first widespread genome editing tool, CRISPR-Cas9. Early life and education Mojica was born in Elche, Spain, on 5 October 1963. He attended Los Andes elementary school, Vázquez de Mella school, and Instituto Carrus high school. He enrolled first at the University of Murcia to study biology and later moved to the University of Valencia ( BS, 1986) and University of Alicante (PhD, 1993). During his doctoral studies, he visited Paris-Sud University. He then received post-doctoral training at the University of Utah and the University of Oxford. Since 1994, Mojica has been a faculty member at the University of Alicante, where he has focused ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Direct Repeat
Direct repeats are a type of genetic sequence that consists of two or more repeats of a specific sequence. In other words, the direct repeats are nucleotide sequences present in multiple copies in the genome. Generally, a direct repeat occurs when a sequence is repeated with the same pattern downstream. There is and no reverse complement associated with a direct repeat. It may or may not have intervening nucleotides. The nucleotide sequence written in bold characters signifies the repeated sequence. ::: ::: Linguistically, a typical direct repeat is comparable to saying "bye-bye". Types There are several types of repeated sequences: *Interspersed (or dispersed) DNA repeats (interspersed repetitive sequences) are copies of transposable elements interspersed throughout the genome. *Flanking (or terminal) repeats (terminal repeat sequences) are sequences that are repeated on both ends of a sequence, for example, the long terminal repeats (LTRs) on retroviruses. Direct terminal repea ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mycobacterium Tuberculosis
''Mycobacterium tuberculosis'' (M. tb), also known as Koch's bacillus, is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, ''M. tuberculosis'' has an unusual, waxy coating on its cell surface primarily due to the presence of mycolic acid. This coating makes the cells impervious to Gram staining, and as a result, ''M. tuberculosis'' can appear weakly Gram-positive. Acid-fastness, Acid-fast stains such as Ziehl–Neelsen stain, Ziehl–Neelsen, or Fluorescence, fluorescent stains such as Auramine O, auramine are used instead to identify ''M. tuberculosis'' with a microscope. The physiology of ''M. tuberculosis'' is highly aerobic organism, aerobic and requires high levels of oxygen. Primarily a pathogen of the mammalian respiratory system, it infects the lungs. The most frequently used diagnostic methods for tuberculosis are the Mantoux test, tuberculin skin test, Acid-Fast Stain, aci ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
