Topic summary
stem-cell lines
Extracted from the Wikipedia article Stem-cell line.
Methods of culture
Stem-cell lines are grown and maintained at specific temperature and atmospheric conditions (37 degrees Celsius and 5% CO2) in incubators. Culture conditions such as the cell growth medium and surface on which cells are grown vary widely depending on the specific stem cell line. Different biochemical factors can be added to the medium to control the cell phenotype—for example to keep stem cells in a pluripotent state or to differentiate them to a specific cell type.