Zymolyase (also known as lyticase, sometimes misspelled as Zymolase) is an enzyme mixture used to degrade the cell wall of
yeast
Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom (biology), kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. They are est ...
and form
spheroplasts.
Essential activities of zymolyase include β-1,3-glucan laminaripentao-hydrolase activity and β-1,3-glucanase activity. A common source of zymolyase is the Actinobacteria ''
Arthrobacter luteus
''Arthrobacter luteus'' (ALU) is a species of gram-positive bacteria in the genus ''Arthrobacter''. ''A. luteus'' is facultatively anaerobic, Pleomorphism (microbiology), pleomorphic, branching, Motility, non-motile, Spore, non-sporulating, non- ...
''.
Commercial sources of zymolyase may have some residual protease activity.
Fungi susceptible to zymolyase can be found in the order
Saccharomycetales
Saccharomycetales belongs to the kingdom of Fungi and the division Ascomycota. It is the only order in the class Saccharomycetes. There are currently 13 families recognized as belonging to Saccharomycetales. GBIF also includes; Alloascoideaceae ...
.
Saccharomycetales genera that zymolyase is known to have activity against include:
''
Eremothecium'', ''
Candida
Candida, or Cándida (Spanish), may refer to:
Biology and medicine
* ''Candida'' (fungus), a genus of yeasts
** Candidiasis, an infection by ''Candida'' organisms
* Malvasia Candida, a variety of grape
Places
* Candida, Campania, a ''comu ...
'', ''
Debaryomyces
''Debaryomyces'' is a genus of yeast
Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom (biology), kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 speci ...
'', ''
Eremothecium'', ''
Endomyces'', ''
Hansenula'', ''
Hanseniaspora'', ''Kloekera'', ''
Kluyveromyces
''Kluyveromyces'' is a genus of ascomycetous yeasts in the family Saccharomycetaceae. Some of the species, such as ''Kluyveromyces marxianus, K. marxianus'', are the teleomorphs of ''Candida (genus), Candida species''.
The genus name of ''Kluyve ...
'', ''
Lipomyces'', ''Metschikowia'', ''
Pichia
''Pichia'' (''Hansenula'' and '' Hyphopichia'' are obsolete synonyms) is a genus of yeasts in the family Pichiaceae with spherical, elliptical, or oblong acuminate cells. ''Pichia'' is a teleomorph, and forms hat-shaped, hemispherical, or roun ...
'', ''Pullularia'', ''
Saccharomyces
''Saccharomyces'' is a genus of fungi that includes many species of yeasts. ''Saccharomyces'' is from Greek σάκχαρον (sugar) and μύκης (fungus) and means ''sugar fungus''. Many members of this genus are considered very important in f ...
'', ''
Saccharomycopsis'', ''Schizosaccahromyces'', ''
Torulopsis''
References
{{reflist
Enzymes
Actinomycetota