A virucide (alternatively spelled viricide) is any physical or chemical agent that deactivates or destroys viruses. The substances are not only virucidal but can be also

bactericidal A bactericide or bacteriocide, sometimes abbreviated Bcidal, is a substance which kills bacteria. Bactericides are disinfectants, antiseptics, or antibiotics. However, material surfaces can also have bactericidal properties based solely on their p ...

fungicidal Fungicides are pesticides used to kill parasitic fungi or their spores. Fungi can cause serious damage in agriculture, resulting in losses of yield and quality. Fungicides are used both in agriculture and to fight fungal infections in animals, ...

, sporicidal or tuberculocidal. Virucides are to be used outside the human body, and as such fall into the category of

disinfectant A disinfectant is a chemical substance or compound used to inactivate or destroy microorganisms on inert surfaces. Disinfection does not necessarily kill all microorganisms, especially resistant bacterial spores; it is less effective than ...

s (applied not to the human body) and

antiseptic An antiseptic ( and ) is an antimicrobial substance or compound that is applied to living tissue to reduce the possibility of sepsis, infection, or putrefaction. Antiseptics are generally distinguished from ''antibiotics'' by the latter's abil ...

s (applied to the surface of skin) for those safe enough. Overall, the notion of virucide differs from an

antiviral drug Antiviral drugs are a class of medication used for treating viral infections. Most antivirals target specific viruses, while a broad-spectrum antiviral is effective against a wide range of viruses. Antiviral drugs are a class of antimicrobials ...

such as

Aciclovir Aciclovir, also known as acyclovir, is an antiviral medication. It is primarily used for the treatment of herpes simplex virus infections, chickenpox, and shingles. Other uses include the prevention of cytomegalovirus infections following tran ...

, which inhibits the proliferation of the virus inside the body. CDC's Disinfection and Sterilization list of ''Chemical Disinfectants'' mentions and discusses substances such as: alcohol, chlorine and chlorine compounds, formaldehyde, glutaraldehyde, hydrogen peroxide, iodophors, ortho-phthalaldehyde (OPA), peracetic acid, peracetic acid and hydrogen peroxide, phenolics, quaternary ammonium compounds, with different, but usually potent ''microbicidal activity''. Other inactivating agents such as

UV light Ultraviolet radiation, also known as simply UV, is electromagnetic radiation of wavelengths of 10–400 nanometers, shorter than that of visible light, but longer than X-rays. UV radiation is present in sunlight and constitutes about 10% of t ...

, metals, and ozone exist.

Definitions

According to the

Centers for Disease Control and Prevention The Centers for Disease Control and Prevention (CDC) is the National public health institutes, national public health agency of the United States. It is a Federal agencies of the United States, United States federal agency under the United S ...

(CDC), a virucide is "An agent that kills viruses to make them noninfective." According to a definition by

Robert Koch Institute The Robert Koch Institute (RKI) is a German federal government agency and research institute responsible for disease control and prevention. It is located in Berlin and Wernigerode. As an upper federal agency, it is subordinate to the Federa ...

Germany and further institutions, virucide means effective against enveloped and non-enveloped viruses. Due to the complexity of the subject, in Germany, Robert-Koch-Institute introduced sub-definitions such as "limited virucidal" or "limited virucidal plus" (translated from German) to differentiate its meaning further. Note that the meaning of virus inactivation or viral clearance is specific for the medical process industry, i.e., to remove HIV from blood.

Functioning

Different substances have interactions between microbicides and viruses such as: * Alteration of the viral envelope * Structural alteration * Alteration of viral markers or * Alteration of the viral genome The exact mechanisms, for example of iodine (PVP-I), are still not clear, but it is targeting the bacterial protein synthesis due to disruption of electron transport, DNA denaturation or disruptive effects on the virus membrane.

Registration

The U.S. Centers for Disease Control and Prevention administers a regulatory framework for disinfectants and sterilants. To earn virucidal registration, extensive data on harder-to-kill viruses demonstrating long-lasting virucidal efficacy need to be provided.

Regulations

* Europe: Biocide products regulation EN 528/2012

Testing

* EN 14476:2019 (suspensions test) * EN 16777:2018 (surfaces test) * EN 1500 (hand rub test) * ISO 18184:2019 (textile products) * ISO 21702:2019 (plastics and non-porous surfaces)

Other related tests

A specific protocol for hand-hygiene testing has been researched and established by microbiologist Prof.

Graham Ayliffe Graham Arthur John Ayliffe (2 March 1926 – 22 May 2017) was a British medical microbiologist and Emeritus Professor in Medical Microbiology, University of Birmingham, United Kingdom. He was instrumental in founding the International Federation ...

Safety

Virucides are not intended for use inside the body, and most are disinfectants that are not intended for use on the surface of the body. Most substances are toxic. None of the listed substances replaces

vaccination Vaccination is the administration of a vaccine to help the immune system develop immunity from a disease. Vaccines contain a microorganism or virus in a weakened, live or killed state, or proteins or toxins from the organism. In stimulating ...

s, if available. Virucides are usually labeled with instructions for safe, effective use. The correct use and scope of disinfectants is very important. Potential serious side-effects with using "quats" (''Quaternary ammonium compounds'') exist, and over-use "can have a negative impact on your customers' septic systems." Mouth-rinsing or gargling can reduce virus load, however experts warn that "Viruses in the nose, lungs or trachea that are released when speaking, sneezing and coughing are unlikely to be reached because the effect is based on physical accessibility of the surface mucous membrane". According to Deutsche Dermatologische Gesellschaft, medical practitioners recommend that disinfectants are gentler on the skin compared to soap-washing. The disinfected hands should then also be creamed to support the regeneration of the skin barrier. Skin care does not reduce the antiseptic effect of the alcoholic disinfectants. The "explosive" use of antibacterial cleansers has led the CDC to monitor substances in adults. On April 5, 2021, a Press Briefing by ''White House COVID-19 Response Team and Public Health Officials'' mentions that "Cleaning with household cleaners containing soap or detergent will physically remove germs from surfaces. This process does not necessarily kill germs, but reduces the risk of infection by removing them. Disinfecting uses a chemical product, which is a process that kills the germs on the surfaces. In most situations, regular cleaning of surfaces with soap and detergent, not necessarily disinfecting those surfaces, is enough to reduce the risk of COVID-19 spread. Disinfection is only recommended in indoor settings — schools and homes — where there has been a suspected or confirmed case of COVID-19 within the last 24 hours. In most situations, regular cleaning of surfaces with soap and detergent, not necessarily disinfecting those surfaces, is enough to reduce the risk of COVID-19 spread." The CDC issued a special report "Knowledge and Practices Regarding Safe Household Cleaning and Disinfection for COVID-19 Prevention" due to the increased number of calls to poison centers regarding exposures to cleaners and disinfectants since the onset of the COVID-19 pandemic, concluding that "Public messaging should continue to emphasize evidence-based, safe cleaning and disinfection practices to prevent SARS-CoV-2 transmission in households, including hand hygiene and cleaning and disinfection of high-touch surfaces." CDC provides a Guideline for Disinfection and Sterilization in Healthcare Facilities.

Microbicidal activity

Each mentioned item in the list has different microbicidal activity, i.e. some viruses can be more or less resistant. For example,

Poliovirus Poliovirus, the causative agent of polio (also known as poliomyelitis), is a serotype of the species '' Enterovirus C'', in the family of '' Picornaviridae''. There are three poliovirus serotypes, numbered 1, 2, and 3. Poliovirus is composed ...

is resistant to a solution of 3% H₂O₂ even after a contact time of 10 minutes, however 7.5% H₂O₂ takes 30 minutes to inactivate over 99.9% of Poliovirus. Generally, hydrogen peroxide is considered as a potent virucide in appropriate concentrations, specifically in other forms such as

gaseous Gas is a state of matter that has neither a fixed volume nor a fixed shape and is a compressible fluid. A ''pure gas'' is made up of individual atoms (e.g. a noble gas like neon) or molecules of either a single type of atom ( elements such as ...

. Another example is

povidone-iodine Povidone-iodine (PVP-I), also known as iodopovidone, is an antiseptic used for skin disinfection before and after surgery. It may be used both to disinfect the hands of healthcare providers and the skin of the person they are caring for. It may ...

(PVP-I), which is found to be effective against

herpes simplex virus Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two members of the Herpesviridae#Human herpesvirus types, human ''Herpesviridae'' family, a set of viruses that produce Viral disease, viral infections in the majority of humans. Both HSV-1 a ...

or SARS-CoV-2, and other viruses, but

coxsackievirus Coxsackieviruses are a few related enteroviruses that belong to the ''Picornaviridae'' family of viral envelope, nonenveloped, linear, positive-sense single-stranded RNA viruses, as well as its genus ''Enterovirus'', which also includes poliovi ...

and polio was rather resistant or less sensitive to inactivation.

SARS-CoV-2 (COVID-19)

In the beginning of the COVID-19 pandemic, then-US President Donald Trump delivered a very dangerous message to the public on the use of disinfectants, which was immediately rejected and refuted by health professionals. In essence, and as mentioned above, virucides are usually toxic depending on concentrations, mixture, etc., and can be deadly not just to viruses, but also if inside a human or animal body or on surface of body. With regards to the COVID-19 pandemic, some of the mentioned agents are still under research about their microbicidal activity and effectivity against SARS-CoV-2, e.g., on surfaces, as mouth-washes, hand-washing, etc. A mixture of 62–71% ethanol, 0.5% hydrogen peroxide or 0.1%

sodium hypochlorite Sodium hypochlorite is an alkaline inorganic chemical compound with the formula (also written as NaClO). It is commonly known in a dilute aqueous solution as bleach or chlorine bleach. It is the sodium salt of hypochlorous acid, consisting of ...

is found to be able to deactivate the novel Coronavirus on surfaces within 1 minute. A 2020

systematic review A systematic review is a scholarly synthesis of the evidence on a clearly presented topic using critical methods to identify, define and assess research on the topic. A systematic review extracts and interprets data from published studies on ...

on hydrogen peroxide (H₂O₂) mouth-washes concludes, that they don't have an effect on virucidal activity, recommending that "dental care protocols during the COVID-19 pandemic should be revised." Additional research with relation to the Coronavirus virucidal efficacy is on-going. Various information and overview of light-based strategies (

UV-C Ultraviolet radiation, also known as simply UV, is electromagnetic radiation of wavelengths of 10–400 nanometers, shorter than that of visible light, but longer than X-rays. UV radiation is present in sunlight and constitutes about 10% of the ...

and other types of light sources; see also

Ultraviolet germicidal irradiation Ultraviolet germicidal irradiation (UVGI) is a disinfection technique employing ultraviolet (UV) light, particularly UV-C (180–280 nm), to kill or inactivate microorganisms. UVGI primarily inactivates microbes by damaging their genetic ma ...

) to combat the COVID-19 pandemic are available. As systematic review of 16 studies by Cochrane on Antimicrobial mouthwashes (gargling) and nasal sprays concludes that "there is currently no evidence relating to the benefits and risks of patients with COVID‐19 using antimicrobial mouthwashes or nasal sprays."

SARS-CoV

Treatment of

SARS-CoV Severe acute respiratory syndrome–related coronavirus (SARSr-CoV or SARS-CoV'', Betacoronavirus pandemicum'')The terms ''SARSr-CoV'' and ''SARS-CoV'' are sometimes used interchangeably, especially prior to the discovery of SARS-CoV-2. This m ...

for 2 min with Isodine ( PVP-I) is found to strongly reduce the virus infectivity.

Research

The International Society of Antimicrobial Chemotherapy (ISAC) is one of the major umbrella organizations for education, research and development in the area of therapy of infections. Its members are national organizations, currently 86 and over 50,000 individual members.

List of virucides

Note that many of the substances, if sold commercially, are usually combinations and mixtures with varying molecular contents. Also note that most products have a limited viricide efficacy. A specific test-protocol is applied. The lists' scope is limited. For further products refer to other lists. Other factors such as stability of the concentrate, application concentration, exposure time, timing of the solution, hydrogen ion concentration (pH value), temperature, etc. play an certain role for the effectivity of a virucide. EPA is providing a public listing called "List N"

General substance listing of active component or compound

* 1-Docosanol * Alcohols: **

Ethanol Ethanol (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound with the chemical formula . It is an Alcohol (chemistry), alcohol, with its formula also written as , or EtOH, where Et is the ps ...

Isopropyl alcohol Isopropyl alcohol (IUPAC name propan-2-ol and also called isopropanol or 2-propanol) is a colorless, flammable, organic compound with a pungent alcoholic odor. Isopropyl alcohol, an organic polar molecule, is miscible in water, ethanol, an ...

** ''n''-propanol *

Benzalkonium chloride Benzalkonium chloride (BZK, BKC, BAK, BAC), also known as alkyldimethylbenzylammonium chloride (ADBAC) is a type of cationic surfactant. It is an organic salt classified as a quaternary ammonium compound. ADBACs have three main categories of use: ...

s, e.g., ** Alkyl dimethyl benzyl ammonium chlorides (C12-16) ** Alkyl dimethyl benzyl ammonium chloride (C14 60%, C16 30%, C12 5%, C18 5%) ** Alkyl dimethyl ethylbenzyl ammonium chloride (C12-14) ** Alkyl dimethyl ethylbenzyl ammonium chlorides (C12-18) *

Bleach Bleach is the generic name for any chemical product that is used industrially or domestically to remove color from (i.e. to whiten) fabric or fiber (in a process called bleaching) or to disinfect after cleaning. It often refers specifically t ...

(

Sodium hypochlorite Sodium hypochlorite is an alkaline inorganic chemical compound with the formula (also written as NaClO). It is commonly known in a dilute aqueous solution as bleach or chlorine bleach. It is the sodium salt of hypochlorous acid, consisting of ...

) **

Sodium hypochlorite washes Sodium hypochlorite washes are skin cleansers formulated with sodium hypochlorite (NaOCl) and surfactants. These cleansing liquids or gels are lathered onto wet skin and rinsed off. They are recommended for inflammatory skin conditions (eczema, ato ...

* Didecyldimethylammonium chloride *

Hand washing Hand washing (or handwashing), also known as hand hygiene, is the act of cleaning one's hands with soap, soap or handwash and water to remove viruses, bacteria, microorganisms, dirt, grease, and other harmful or unwanted substances stuck to th ...

(see also Surfactants) ** Hand washing is a mechanical process of removing germs and viruses, and chemicals. ** Hand washing with, e.g., ethanol added to a hand disinfectant shows virucidal effects, but caution is given (small children) and it is not recommended over "proper hand washing". ** Hand gels are often found to not comply with EN 1500 standards to meet antimicrobial efficacy. ** Prof.

's hand-cleaning and disinfection technique is promoted nowadays by the WHO and is similar to German standard DIN EN 1500 (hygienic hand disinfection). *

Hydrogen peroxide Hydrogen peroxide is a chemical compound with the formula . In its pure form, it is a very pale blue liquid that is slightly more viscosity, viscous than Properties of water, water. It is used as an oxidizer, bleaching agent, and antiseptic, usua ...

* Oral rinse (see Cochrane systematic review in case of SARS-CoV-2) **

Chlorhexidine Chlorhexidine is a disinfectant and antiseptic which is used for skin disinfection before surgery and to disinfect surgical instruments. It is also used for cleaning wounds, preventing dental plaque, treating yeast infections of the mouth, and ...

(CHX) - mainly against ''enveloped'' viruses. ** Dequalinium *

Povidone-iodine Povidone-iodine (PVP-I), also known as iodopovidone, is an antiseptic used for skin disinfection before and after surgery. It may be used both to disinfect the hands of healthcare providers and the skin of the person they are caring for. It may ...

(Isodine, PVP-I), ** High potency for virucidal activity has been observed against viruses of significant global concern, including hepatitis A and influenza, as well as the Middle-East Respiratory Syndrome and Sudden Acute Respiratory Syndrome coronaviruses. ** Application types and names: Isodine, Scrub, Isodine Nodo Fresh *

Surfactant Surfactants are chemical compounds that decrease the surface tension or interfacial tension between two liquids, a liquid and a gas, or a liquid and a solid. The word ''surfactant'' is a Blend word, blend of "surface-active agent", coined in ...

s **

Soap Soap is a salt (chemistry), salt of a fatty acid (sometimes other carboxylic acids) used for cleaning and lubricating products as well as other applications. In a domestic setting, soaps, specifically "toilet soaps", are surfactants usually u ...

Triton X-100 Triton X-100 (''n'') is a nonionic surfactant that has a hydrophilic polyethylene oxide chain (on average it has 9.5 ethylene oxide units) and an aromatic hydrocarbon lipophilic or hydrophobic group. The hydrocarbon group is a 4-( 1,1,3,3-tetr ...

Example products

* Betadine products and medical variants by Avrio Health (part of

Purdue Pharma Purdue Pharma L.P., formerly the Purdue Frederick Company (1892–2019), was an American privately held pharmaceutical company founded by John Purdue Gray. It was sold to Arthur Sackler, Arthur, Mortimer Sackler, Mortimer, and Raymond Sackler in 1 ...

) ** Ingredients: Povidon-iodine etc. ** As of June 2021, not recommended by manufacturer to "kill" coronaviruses. * Bleach products: **

Clorox The Clorox Company (formerly Clorox Chemical Company) is an American multinational manufacturer and marketer of consumer and professional products. As of 2024, the Oakland, California-based company had approximately 8,000 employees worldwide. N ...

** Cyosan ** Zonrox Bleach *

Henkel Henkel AG & Co. KGaA, commonly known as Henkel, is a German multinational chemical and consumer goods company headquartered in Düsseldorf, Germany. Founded in 1876, the DAX company is organized into two globally operating business units (Cons ...

products: ** biff Hygiene Total *** Ingredients:

and

formic acid Formic acid (), systematically named methanoic acid, is the simplest carboxylic acid. It has the chemical formula HCOOH and structure . This acid is an important intermediate in chemical synthesis and occurs naturally, most notably in some an ...

*** Tested against SARS-CoV-2 according to producer statement on website. ** Bref Power Bakterien & Schimmel **

Purex PUREX (plutonium uranium reduction extraction) is a chemical method used to purify fuel for nuclear reactors or nuclear weapons. It is based on liquid–liquid extraction ion-exchange. PUREX is the '' de facto'' standard aqueous nuclear reproc ...

* Heitmann Hygiene & Care products: ** Universal Hygiene Laundry Rinse 1.5 *** Ingredients: Didecyldimethylammonium chloride ** Hygiene Spray *** Ingredients: Ethanol, 2-propanol *** According to manufacturer is effective against SARS-CoV-2 *

Listerine Listerine (, ) is an American brand of antiseptic mouthwash that is promoted with the slogan "Kills germs that cause bad breath". Named after Joseph Lister, who pioneered antiseptic surgery at the Glasgow Royal Infirmary in United Kingdom, L ...

** Ingredients: Alcohol, sodium fluoride, essential oils (specifically in case of management of inflammatory

periodontal disease Periodontal disease, also known as gum disease, is a set of inflammatory conditions affecting the tissues surrounding the teeth. In its early stage, called gingivitis, the gums become swollen and red and may bleed. It is considered the main c ...

s) ** Unknown or limited virucidal activity *

Lysol Lysol (, ; spelled Lizol in India) is an American brand of cleaning and Disinfectant, disinfecting products distributed by Reckitt, which markets the similar Dettol or Sagrotan in other markets. The line includes liquid solutions for hard and s ...

** Ingredient:

** Some of the products having been tested against SARS-CoV-2. * Sterillium ** Ingredients: 1-Propanol,

2-propanol Isopropyl alcohol (IUPAC name propan-2-ol and also called isopropanol or 2-propanol) is a colorless, flammable, organic compound with a pungent alcoholic odor. Isopropyl alcohol, an organic polar molecule, is miscible in water, ethanol, an ...

and mecetronium ethylsulfate ** By former Bode Chemie, now Hartmann AG, one of Germany's major health-care brands available in 50 countries, and according to website "the world's most scientifically researched hand disinfectant with approximately 60 scientific publications in trade journals in 2015."

Other substances, drugs, proteins, therapeutics, research-level topics

Antimicrobial peptides Antimicrobial peptides (AMPs), also called host defence peptides (HDPs) are part of the innate immune response found among all classes of life. Fundamental differences exist between Prokaryote, prokaryotic and eukaryota, eukaryotic cells that may ...

* Auriclosene (NVC-422) - ''see also

Keratoconjunctivitis Keratoconjunctivitis is a term used to describe inflammation of both the cornea (the clear, front part of the eye) and the conjunctiva (the thin, transparent membrane covering the white part of the eye and lining the inside of the eyelids). This co ...

'' *

Bacteriocin Bacteriocins are proteinaceous or peptide, peptidic toxins produced by bacteria to inhibit the growth of similar or closely related bacterial strain(s). They are similar to yeast and paramecium killing factors, and are structurally, functionally ...

Chlorine dioxide Chlorine dioxide is a chemical compound with the formula ClO2 that exists as yellowish-green gas above 11 °C, a reddish-brown liquid between 11 °C and −59 °C, and as bright orange crystals below −59 °C. It is usually ...

Copper alloys Copper alloys are metal Alloy, alloys that have copper as their principal component. They have high resistance against corrosion. Of the large number of different types, the best known traditional types are bronze, where tin is a significant addi ...

* CLR01 ( Molecular tweezers) found to inhibit Ebola, Zika or possibly SARS-CoV-2 * Cyanovirin-N * General so called "

Drug repurposing A drug is any chemical substance other than a nutrient or an essential dietary ingredient, which, when administered to a living organism, produces a biological effect. Consumption of drugs can be via inhalation, injection, smoking, ingestion, ...

" for example in case of SARS-CoV-2/COVID-19 * Griffithsin *

Interferon Interferons (IFNs, ) are a group of signaling proteins made and released by host cells in response to the presence of several viruses. In a typical scenario, a virus-infected cell will release interferons causing nearby cells to heighten ...

Nanomedicine Nanomedicine is the medical application of nanotechnology, translating historic nanoscience insights and inventions into practical application. Nanomedicine ranges from the medical applications of nanomaterials and biological devices, to n ...

s * "Novel Anti-Infectives" research by Helmholtz Centre for Infection Research *

Peracetic acid Peracetic acid (also known as peroxyacetic acid, or Percidine) is an organic compound with the formula CH3CO3H. This peroxy acid is a colorless liquid with a characteristic acrid odor reminiscent of acetic acid. It can be highly corrosive. Perac ...

Scytovirin Scytovirin is a 95-amino acid antiviral protein isolated from the cyanobacteria '' Scytonema varium''. It has been cultured in ''E. coli'' and its structure investigated in detail. Scytovirin is thought to be produced by the bacteria to protect its ...

Urumin Urumin is a naturally occurring 27-amino acid virucidal host defense peptide against the human influenza A virus. It was discovered and isolated from the skin of '' Hydrophylax bahuvistara'', a species of frog found in South India, by a team of ...

Agricultural, veterinary

* Vanproz V-Bind * Virkon * Turnip yellow mosaic virus (TuYV) resistant products, such as

Bayer AG Bayer AG (English: , commonly pronounced ; ) is a German multinational pharmaceutical and biotechnology company and is one of the largest pharmaceutical companies and biomedical companies in the world. Headquartered in Leverkusen, Bayer's ...

's DK Excited