V8 Medium
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V8 medium is a kind of
agar Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from " ogonori" and " tengusa". As found in nature, agar is a mixture of two components, t ...
medium Medium may refer to: Aircraft *Medium bomber, a class of warplane * Tecma Medium, a French hang glider design Arts, entertainment, and media Films * ''The Medium'' (1921 film), a German silent film * ''The Medium'' (1951 film), a film vers ...
which is used to cultivate the spore of
Phytophthora sojae ''Phytophthora sojae'' is an oomycete and a soil-borne plant pathogen that causes stem and root rot of soybean. This is a prevalent disease in most soybean growing regions, and a major cause of crop loss. In wet conditions the pathogen produces ...
. Phytophthora sojae causes
soybean The soybean, soy bean, or soya bean (''Glycine max'') is a species of legume native to East Asia, widely grown for its edible bean. Soy is a staple crop, the world's most grown legume, and an important animal feed. Soy is a key source o ...
blight Blight is a specific symptom affecting plants in response to infection by a pathogenic organism. Description Blight is a rapid and complete chlorosis, browning, then death of plant tissues such as leaves, branches, twigs, or floral organs. A ...
(Phytophthora root rot of soybeans). Soybean blight can affect the output and quality of soybeans seriously. The spore of phytophthora sojae is difficult to culture in potato dextrose agar; it is generally cultured by V8 medium and lima bean agar at home and abroad. V8 medium can cultivate, separate, reproduce and conserve many kinds spore of phytophthora sojae, but it is not suitable for cultivating the pathogen of potato late blight.


References

* Sinclair, J.B.; Dhingra, O.D. (1995). Basic Plant Pathology Methods. Taylor & Francis. p. 36. . * Santamaria, L. (2007). Evaluation of Lima Bean (Phaseolus Lunatus) Germplasm for Resistance to Downy Mildew, and Epidemiological and Biological Studies of Its Causal Agent, Phytophthora Phaseoli. University of Delaware. pp. 39–40. . {{Growth media Cell culture media Microbiological media