Type I site-specific deoxyribonuclease (, ''type I restriction enzyme'', ''deoxyribonuclease (ATP- and S-adenosyl-L-methionine-dependent)'', ''restriction-modification system'', ''deoxyribonuclease (adenosine triphosphate-hydrolyzing)'', ''adenosine triphosphate-dependent deoxyribonuclease'', ''ATP-dependent DNase'', ''type 1 site-specific deoxyribonuclease'') is an
enzyme
An enzyme () is a protein that acts as a biological catalyst by accelerating chemical reactions. The molecules upon which enzymes may act are called substrate (chemistry), substrates, and the enzyme converts the substrates into different mol ...
.
This enzyme
catalyses the following
chemical reaction
A chemical reaction is a process that leads to the chemistry, chemical transformation of one set of chemical substances to another. When chemical reactions occur, the atoms are rearranged and the reaction is accompanied by an Gibbs free energy, ...
: Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed
They have an absolute requirement for ATP (or dATP) and
S-adenosyl-L-methionine
''S''-Adenosyl methionine (SAM), also known under the commercial names of SAMe, SAM-e, or AdoMet, is a common cosubstrate involved in methyl group transfers, transsulfuration, and aminopropylation. Although these anabolic reactions occur throug ...
.
See also
*
Restriction enzyme
A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class o ...
References
External links
*
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EC 3.1.21