Type III site-specific deoxyribonuclease (, ''type III restriction enzyme'', ''restriction-modification system'') is an
enzyme
An enzyme () is a protein that acts as a biological catalyst by accelerating chemical reactions. The molecules upon which enzymes may act are called substrate (chemistry), substrates, and the enzyme converts the substrates into different mol ...
.
This enzyme
catalyses the following
chemical reaction
A chemical reaction is a process that leads to the chemistry, chemical transformation of one set of chemical substances to another. When chemical reactions occur, the atoms are rearranged and the reaction is accompanied by an Gibbs free energy, ...
: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-
phosphates
Phosphates are the naturally occurring form of the element phosphorus.
In chemistry, a phosphate is an anion, salt, functional group or ester derived from a phosphoric acid. It most commonly means orthophosphate, a derivative of orthophosphor ...
This group of enzymes has an absolute requirement for ATP, but does not hydrolyse it.
See also
*
Restriction enzyme
A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class o ...
References
External links
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EC 3.1.21