Two Brothers may refer to:

Film and television

* ''Two Brothers'' (1929 film), a German silent film directed by Mikhail Dubson * ''Two Brothers'' (2004 film), a French-British film directed by Jean-Jacques Annaud * ''The Two Brothers'' (film), a 1910 American short Western film directed by D. W. Griffith * Do Bhai (disambiguation) (), various Indian films * Two Brothers Pictures, a British television production company founded in 2014

Literature and folklore

*'' Tale of Two Brothers'', an ancient Egyptian story *'' The Two Brothers'', a German fairy tale * ''The Two Brothers'' (novel), or ''La Rabouilleuse'', an 1842 novel by Honoré de Balzac *''Dois Irmãos'' (''Two Brothers''), a 2000 novel by Milton Hatoum

* " The Twa Brothers", a traditional ballad * ''Two Brothers'' (album), a 2001 album by Boxhead Ensemble

* Two Brothers, U.S. Virgin Islands, a settlement on the island of Saint Croix * Two Brothers Rocks, a rock formation in the Ionian Sea near Syracuse, Sicily

* ''Two Brothers'' (ship), a 19th-century Nantucket whaleship * ''Two Brothers'' (video game), a 2013 action role-playing game * Two Brothers Brewing, an American microbrewery * Two Brothers Roundhouse, on the former Chicago & Aurora Railroad, in Aurora, Illinois, U.S. {{disambiguation, geo