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Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.
Tryptone is commonly used in
Seed germination and further development of plantlets of ''Paphiopedilum ciliolare'' Pfitz. ''in vitro''.
''Scientia Horticulturae'', 34(1), 139-153.
Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.
Tryptone is commonly used in microbiology
Microbiology () is the scientific study of microorganisms, those being unicellular (single cell), multicellular (cell colony), or acellular (lacking cells). Microbiology encompasses numerous sub-disciplines including virology, bacteriology, prot ...
to produce lysogeny broth (LB) for the growth of ''E. coli
''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus ''Escher ...
'' and other microorganisms. It provides a source of amino acids for the growing bacteria. Tryptone is similar to casamino acid Casamino acids is a mixture of amino acids and some very small peptides obtained from acid hydrolysis of casein. It is typically used in microbial growth media. It has all the essential amino acids except tryptophan, which is destroyed by digestio ...
s, both being digests of casein, but casamino acids can be produced by acid hydrolysis and typically only have free amino acids and few peptide chains; tryptone by contrast is the product of an incomplete enzymatic hydrolysis with some oligopeptides present.
Tryptone is also a component of some germination media used in plant propagation.Pierik, R. L. M., et al. (1988)Seed germination and further development of plantlets of ''Paphiopedilum ciliolare'' Pfitz. ''in vitro''.
''Scientia Horticulturae'', 34(1), 139-153.
See also
*Albumose
A proteose is any of various water-soluble compounds that are produced during in-vitro or in-vivo hydrolytic breakdown of proteins a little before producing amino acids. It forms after breaking down of polypeptides by proteases such as gastric pep ...
* Trypticase soy agar
References
Peptides Microbiological media ingredients {{Microbiology-stub