Tree Injection
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Trunk injection or endotherapy also known as vegetative endotherapy, is a method of target-precise application of
pesticides Pesticides are substances that are used to pest control, control pest (organism), pests. They include herbicides, insecticides, nematicides, fungicides, and many others (see table). The most common of these are herbicides, which account for a ...
, plant resistance activators, or fertilizers into the
xylem Xylem is one of the two types of transport tissue (biology), tissue in vascular plants, the other being phloem; both of these are part of the vascular bundle. The basic function of the xylem is to transport water upward from the roots to parts o ...
vascular tissue of a tree with the purpose of protecting the tree from pests, or to inject
nutrients A nutrient is a substance used by an organism to survive, grow and reproduce. The requirement for dietary nutrient intake applies to animals, plants, fungi and protists. Nutrients can be incorporated into cells for metabolic purposes or excret ...
to correct for nutrient deficiencies. This method largely relies on harnessing the tree's vascular system to translocate and distribute the active compounds into the wood, canopy and roots where protection or nutrition is needed. Trunk injection/endotherapy is currently the most popular method for control of damaging insects, pathogens, and nematodes in landscape tree care. Endotherapy is the concept when treatments using the appropriate material formulation are carried out from the inside out through xylem translocation in the trunk/stem of plants during the photosynthetic cycle. Trunk injection is an older term that only reflects how the technique is performed.


Description

Endotherapy has been developed primarily for use on large size trees and in proximity of urban areas where ground- and air-spray applications are impractical due to substantial drift-driven pesticide losses or not allowed due to potential human exposure. However, the prime driver of tree injection use has been a wide spread need for control of many invasive tree pathogens and insects pests. The most infamous examples are that of fungi in the genus ''Ophiostoma'' that cause Dutch Elm Disease (DED) and the insect known as the
emerald ash borer The emerald ash borer (''Agrilus planipennis''), also known by the abbreviation EAB, is a green buprestid or jewel beetle native to north-eastern Asia that feeds on ash trees, ash species (''Fraxinus'' spp.). Females lay eggs in bark crevices o ...
(''Agrilus planipennis'') which have specific biologies that lead to severe internal damage of wood and thus tree death, and which make their management extremely difficult or inefficient with classical pesticide application methods. Endotherapy for tree protection is viewed as environmentally safer alternative for pesticide application since the compound is delivered within the tree, thus allowing for selective exposure to plant pests. In landscapes and urban zones trunk injection significantly reduces the non-target exposure of water, soil, air, and wildlife to pesticides and fertilizers. In the last 20 years, tree injection is gaining momentum with the development and availability of new, efficient injection devices and injectable and xylem mobile formulations of pesticides, biopesticides and nutrients. Endotherapy works by adding a
water soluble In chemistry, solubility is the ability of a substance, the solute, to form a solution with another substance, the solvent. Insolubility is the opposite property, the inability of the solute to form such a solution. The extent of the solubi ...
chemical formulation directly into the lower trunk of the tree structure.


Applications

A number of newly occurring and fast spreading invasive insect pests and diseases such as Polyphagous Shot Hole Borer (PSHB) ('' Euwallacea'' spp.), which can vector plant pathogenic fungus ''
Fusarium ''Fusarium'' (; ) is a large genus of filamentous fungi, part of a group often referred to as hyphomycetes, widely distributed in soil and associated with plants. Most species are harmless saprobes, and are relatively abundant members of the s ...
euwallaceae'', and Sudden Oak Death (SOD) caused by an
Oomycete The Oomycetes (), or Oomycota, form a distinct phylogenetic lineage of fungus-like eukaryotic microorganisms within the Stramenopiles. They are filamentous and heterotrophic, and can reproduce both sexually and asexually. Sexual reproduction o ...
''
Phytophthora ramorum ''Phytophthora ramorum'' is the oomycete known to cause the disease sudden oak death (SOD). The disease kills oak and other species of trees and has had devastating effects on the oak populations in California and Oregon, as well as being present ...
'', establish the use of endotherapy as the most efficient tree protection technique in landscapes and urban forestry. In the past and recently, endotherapeutic treatment using agriculture products has been investigated in the perennial trees for control of pathogens and insect pests on fruit tree crops. The most investigated are diseases and pests of avocado, coconut palm, apple, and grapevine, such as Phytophthora root rot of avocado ''
Phytophthora cinnamomi ''Phytophthora cinnamomi'', also known as cinnamon fungus, is a soil-borne water mould that produces an infection which causes a condition in plants variously called "dieback", "root rot", or (in certain '' Castanea'' species), "ink disease". O ...
'' and avocado
thrips Thrips (Order (biology) , order Thysanoptera) are minute (mostly long or less), slender insects with fringed wings and unique asymmetrical mouthparts. Entomologists have species description , described approximately 7,700 species. They fly on ...
''Scirtothrips perseae'',
fire blight Fire blight, also written fireblight, is a contagious disease affecting apples, pears, and some other members of the family Rosaceae. It is a serious concern to apple and pear producers. Under optimal conditions, it can destroy an entire orchard ...
'' Erwinia amylovora'' and
apple scab Apple scab is a common disease of plants in the rose family (Rosaceae) that is caused by the ascomycete fungus ''Venturia inaequalis''. While this disease affects several plant genera, including '' Sorbus, Cotoneaster,'' and '' Pyrus'', it is ...
''
Venturia inaequalis ''Venturia inaequalis'' is an ascomycota, ascomycete fungus that causes the apple scab disease. Systematics ''Venturia inaequalis'' anamorphs have been described under the names ''Fusicladium dendriticum'' and ''Spilocaea pomi''. Whether ''V. in ...
'', oblique banded leaf roller '' Choristoneura rosaceana'' and
codling moth The codling moth (''Cydia pomonella'') is a member of the Lepidopteran family Tortricidae. They are major pests to agricultural crops, mainly fruits such as apples and pears, and a codling moth larva is often called an " apple worm". Along with ...
''Cydia pomonella'', and grapevine downy mildew '' Plasmopara viticola'' and powdery mildew ''
Uncinula necator ''Uncinula necator'' (syn. ''Erysiphe necator'') is a fungus that causes powdery mildew of grape. It is a common pathogen of Vitis species, including the wine grape, ''Vitis vinifera''. The fungus is believed to have originated in North Americ ...
''. Apple trees are especially interesting as a research model in agriculture since it is known that apple production requires intensive spray schedules for control of pathogenic fungus '' V. inaequalis'' with as many as 15-22 sprays of fungicides per season in humid climate. Endotherapy of pesticides is considered as an option for precise compound delivery which will reduce the negative impact of drift-driven pesticide losses in the environment, that occur after aerial or ground spray applications of pesticides. Besides negative consequences of frequent pesticide applications in the environment, trunk injection of grapevines is investigated in viticulture for control of pathogens with difficult biologies, such as ''
Xylella fastidiosa ''Xylella fastidiosa'' is an aerobic, Gram-negative bacterium of the genus ''Xylella''. It is a plant pathogen, that grows in the water transport tissues of plants ( xylem vessels) and is transmitted exclusively by xylem sap-feeding insects suc ...
'', which infect and destroy woody tissues and that cannot be controlled efficiently by canopy spray applications of fungicides or bactericides. To increase the efficiency of injected compounds in trees and vines, important considerations are plant anatomy, weather and soil conditions, tree physiology processes, spatial and temporal distribution of injected compound, and the chemical properties of injected compound and formulation.


References

{{Reflist, 30em Forestry