Thermococcus Celer

''Thermococcus celer'' is a Gram-negative, spherical-shaped archaeon of the genus ''Thermococcus''.Achenbach-Richter, L., R. Gupta, W. Zillig, C. R. Woese. 1988. Rooting the Archaebacterial Tree: The Pivitol Role of Thermococcus celer in Archaebacterial Evolution. Syst. Appl. Microbial. 10:231-240. Print. The discovery of ''T. celer'' played an important role in rerooting the tree of life when ''T. celer'' was found to be more closely related to methanogenic Archaea than to other phenotypically similar thermophilic species. ''T. celer'' was the first archaeon discovered to house a circularized genome. Several type strains of ''T. celer'' have been identified: Vu13, ATCC 35543, and DSM 2476.

Isolation

''T. celer'' was discovered by Dr. Wolfram Zillig in 1983. The organism was isolated on the beaches of Vulcano, Italy, from a sulfur-rich shallow volcanic crater.
Original samples were isolated from the depths of the marine holes and inoculated into 10-ml anaerobic tubes.Zillig, W., K. O. Stetter, W. Schafer, D. Janekovic, S. Wunderl, I. Holz, and P. Palm. "Thermoproteales: Novel Order of Archaebacteria." Zentralblatt für Bakteriologie Mikrobiologie und Hygiene 2 (1981): 205-27. Print. The tubes contained 100 mg of elemental sulfur and a solution of 95% N₂ and 5% H₂S. The pH was subsequently adjusted to a range of 5-6 through the addition of CaCO₃. To ensure that no oxygen had permeated the sample, researchers used the oxygen indicator resazurin. Growth was achieved by enrichment with Brock’s ''Sulfolobus'' medium, which contains elemental sulfur and yeast, both of which are required by ''T. celer'' for optimal growth. Following enrichment, the samples were plated onto polyacrylamide gel and then incubated at 85°C in an anaerobic environment. Once colony growth had been observed, the cells were subjected to centrifugation prior to purification in a TA buffer solution (0.05 mol/L Tris HCl, 0.022 mol/L NH₄Cl, 0.01 mol/L β-mercaptoethanol).

Taxonomy and phylogeny

Following Sanger sequencing of the 16s rRNA, both parsimony and distance matrix analyses were performed to determine the position of ''T. celer'' on the tree of life. ''T. celer'' was found to be more closely related to the methanogenic archaebacteria than the thermophilic archaebacteria. This discovery resulted in a rerooting of the archaebacterial tree and subsequently placed ''T. celer'' in a clade with the methanogens based upon their close phylogenetic relationship. This placement was further supported following analysis of the organizational genome structure of the both species’ rRNA genes. Both ''Thermococcus'' and methanogenic archaebacteria have a tRNA spacer gene located between the 16s rRNA gene and 23s This spacer gene is not found in any other thermophilic archaebacteria species.

''T. celer'' is related to ''Pyrococcus woesei">RNA gene. This spacer gene is not found in any other thermophilic archaebacteria species.

''T. celer'' is related to ''Pyrococcus woesei'', both belonging to the order Thermococcales.Blamey, J., M. Chiong, C. Lopez, and E. Smith. 1999. Optimization of the growth conditions of the extremely thermophilic microorganisms Thermococcus celer and Pyrococcus woesei. Journal of Microbiological methods. Vol: 38:1-2:169-175. Print. Both are strictly anaerobic and sulphur-reducing. ''T. celer'' also shares a close relationship with ''Thermococcus litoralis'', both belonging to the same genus, but ''T. celer'' has shown to be much more Sulphur-dependent than ''T. littorals''.

'' T. celer'' is currently classified as a thermophilic Archaeon. Since the discovery of ''T. celer'', the term archaebacteria has been replaced with Archaea as to reflect the most current phylogenetic relationships discovered between the organisms.

Characterization

''Thermococcus'' is constructed from two Greek nouns: ''therme'' (Greek, meaning heat), and ''kokkos'' (Greek, meaning grain or seed). ''Celer'' is derived from the Greek, meaning fast, in reference to the species' high growth rates.

Morphology

''T. celer'' is a Gram-negative, spherical organism around 1 μm diameter. Observation using electron microscopy revealed that '' T. celer '' uses a monopolar polytrichous flagellum for movement. During replication, ''T. celer'' is condensed to a diploform shape as seen by phase contrast microscopy.

The ''T. celer'' plasma membrane possesses large amounts of glycerol diether lipids compared to relatively small amounts of diglycerol tetraether lipids.Boone, David R., and Richard W. Castenholz. Bergey's Manual® of Systematic Bacteriology Volume One The Archaea and the Deeply Branching and Phototrophic Bacteria. Second ed. New York, NY: Springer New York, 2001. 341-344. Print.
Within glycerol diether lipids, phytanyl (C₂₀) is the hydrocarbon component, and within diglycerol tetraether lipids, biphytanyl (C₄₀) is the hydrocarbon component. The cell wall, or S-layer, of ''T. celer'' functions as protection from cell lysis as a result of changes in osmotic gradients. The envelope S-layer consists of glycoprotein subunits arranged into a two-dimensional paracrystalline hexagonal structure.
The ''T. celer'' cell envelope lacks muramic acid, indicating resistance to penicillin and vancomycin.

Metabolism

''T. celer'' is a strict anaerobe that uses organotrophic metabolism in the form of peptides (i.e. from yeast extract, peptone, or tryptone) and proteins (i.e. casein) as a carbon source which are oxidized to carbon dioxide via sulphur respiration. ''T. celer'' is unable to use carbohydrates as a carbon source and is considered a sulphur-dependent organism, as it depends upon the reduction of sulphur to hydrogen sulfide for optimal growth. Though it is less efficient, ''T. celer'' is also able to use fermentation. Unlike most prokaryotes, ''T. celer'' is able to perform respiration via the Embden–Meyerhof pathway ( glycolysis), though it uses an alternative route. Gadd, Geoffrey M. "EMP Pathway." Bacterial Physiology and Metabolism. By Byung H. Kim. New York: Cambridge, 2008. 65-67. Print.

Ecology

Characteristic of the hyperthermophilic species, ''T. celer'' thrives in extremely hot temperatures. More specifically, it is found only in sulphur-rich, shallow volcanic craters of Vulcano, Italy. Temperatures in this specific habitat reach up to 90 °C The maximum temperature at which ''T. celer'' can grow at is 93 °C, optimum growth temperature being 88 °C. It grows best at a pH of 5.8, implying that it is mildly acidophilic. Optimal growth is also dependent on the presence of a NaCl concentration of 40 g/L, further demonstrating the high level of adaptation ''T. celer'' has evolved for its thermal marine environment.

Genomics

Construction of a physical map of ''T. celer'' Vu13 by way of restriction enzyme fragments revealed a length of 1,890 + 27 kilobases (kb). The molecular ratio of guanine to cytosine bases is roughly 56.6%. This value was determined by averaging both the GC-content acquired by high-performance liquid chromatography and melting point (T_M) calculations. ''T. celer'' is considered to be one of the slowest evolving archaeon species, indicating that the genome could be used as a model organism for those studying early genome characteristics.

In 1989, ''T. celer'' was the first archaeon discovered to house a circularized genome. Genome shape was determined through three separate experiments, all using restriction enzymes. The ''T. celer'' genome was digested with restriction enzymes ''Nhe'', ''Spe'', and ''Xba''. Following digestion, hybridization analyses were used to determine genome shape. Probes were synthesized from cloned genes of the 16S rRNA and 23S rRNA. Both ''Spe'' and ''Nhe'' produced five fragments, all of similar shape and size. Digestion with ''Xba'' produced eight fragments. Using overlap patterns, the shape of the genome was determined to be circular.

Application

The domain Archaea is currently split into three major groups consisting of the extreme thermophiles, the extreme halophiles, and the extreme thermophiles that are able to reduce sulfur (methanogens). These three groups are not believed to have arisen independently, but instead evolved from one to another.

The discovery of ''T. celer'' turned the position of the archaebacterial phylogenetic tree. It was discovered to share a closer phylogenetic relationship with methanogenic archaebacteria, as opposed its phenotypic analogue, extremely thermophilic archaebacteria. This discovery was made through sequence analysis of the 16S rRNA and resulted in a rerooting of the phylogenetic tree.

This discovery suggests that extreme thermophiles could be the earliest archaeon ancestor when considering their slow evolution patterns, as well as the distribution of extreme thermophiles into both their own grouping, as well as that of the methanogens.

References

Further reading

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External links

Type strain of ''Thermococcus celer'' at Bac''Dive'' - the Bacterial Diversity Metadatabase

{{Taxonbar, from=Q7783145

Euryarchaeota
Archaea described in 1983