Variable number of tandem repeat locus (
VNTR locus) is any
DNA sequence that exist in multiple copies strung together in a variety of tandem lengths. The number of repeat copies present at a locus can be visualized by means of a Multi-locus or
Multiple Loci VNTR Analysis
Multiple loci VNTR analysis (MLVA) is a method employed for the genetic analysis of particular microorganisms, such as pathogenic bacteria, that takes advantage of the polymorphism of tandemly repeated DNA sequences. A " VNTR" is a "variable-numbe ...
(
MLVA
Multiple loci VNTR analysis (MLVA) is a method employed for the genetic analysis of particular microorganisms, such as pathogenic bacteria, that takes advantage of the polymorphism of tandemly repeated DNA sequences. A " VNTR" is a "variable-numbe ...
). In short,
oligonucleotide
Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics. Commonly made in the laboratory by solid-phase chemical synthesis, these small bits of nucleic acids ...
primers are developed for each specific tandem repeat locus, followed by
PCR PCR or pcr may refer to:
Science
* Phosphocreatine, a phosphorylated creatine molecule
* Principal component regression, a statistical technique
Medicine
* Polymerase chain reaction
** COVID-19 testing, often performed using the polymerase chain r ...
and
agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of ...
. When the length of the repeat and the size of the flanking regions is known, the number of repeats can be calculated. Analysis of multiple loci will result in a
genotype
The genotype of an organism is its complete set of genetic material. Genotype can also be used to refer to the alleles or variants an individual carries in a particular gene or genetic location. The number of alleles an individual can have in a ...
.
References
Genetics
{{Genetics-stub