Midline-2 is a
protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residue (biochemistry), residues. Proteins perform a vast array of functions within organisms, including Enzyme catalysis, catalysing metab ...
that in humans is encoded by the ''MID2''
gene
In biology, the word gene has two meanings. The Mendelian gene is a basic unit of heredity. The molecular gene is a sequence of nucleotides in DNA that is transcribed to produce a functional RNA. There are two types of molecular genes: protei ...
.
Function
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Recent reports indicate the involvement of MID2 in cytokinesis
.MID2 (TRIM1) ubiquitinates
Sperm-associated antigen 5 (Astrin) on K409, further promoting its degradation and proper cytokinesis.
In contrary, depletion of MID2 (TRIM1) stabilizes
Sperm-associated antigen 5 (Astrin) whose inappropriate accumulation at the midbody triggers cytokinetic arrest, multinucleated cells, and cell death.
Interactions
MID2 has been shown to
interact
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with
MID1.
MID2 (TRIM1) interacts with
Leucine-rich repeat kinase 2 (LRRK2), which is often subject to missense mutations in familial Parkinson's disease (PD).
MID2 (TRIM1) specifically binds to the flexible regulatory loop of LRRK2
853–981.
MID2 (TRIM1) recruits
LRRK2 to the microtubule cytoskeleton where MID2 (TRIM1) ubiquitinates LRRK2 targeting it for proteasomal degradation.
References
Further reading
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