TREX1

Three prime repair exonuclease 1 is an enzyme that in humans is encoded by the ''TREX1'' gene.

Function

This gene encodes the major 3'->5' DNA exonuclease in human cells. The protein is a non-processive exonuclease that may serve a proofreading function for a human DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A-mediated cell death. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, RVCL (Retinal Vasculopathy with Cerebral Leukodystrophy), and Cree encephalitis. Multiple transcript variants encoding different isoforms have been found for this gene.

Clinical relevance

Mutations within the ''TREX1'' gene cause familial chilblain lupus. The ''TREX1'' polymorphisms confer susceptibility to systemic lupus erythematosus. Missense mutations of the ''TREX1'' gene significantly downregulate its exonucleolytic capacity and result in the accumulation of nucleic acids. The build-up of the nucleic acids within the cytoplasm stimulates type-I interferon responses that could trigger autoimmune responses. The region containing the ''TREX1'' gene (3p21.31) has been linked to COVID-19 severity in a recent genome-wide association study. This might explain the occurrence of chilblain like lesions in patients infected with SARS-CoV-2.

TREX1 helps HIV‑1 to evade cytosolic sensing by degrading viral cDNA in the cytoplasm

Mutations in TREX1 can give cause failure to appropriately remove ribonucleotides misincorporated into DNA. The removal process is ordinary performed by ribonucleotide excision repair. In humans, a defect in this process can give rise to Aicardi-Goutieres syndrome involving microcephaly and neuroinflammation.

Homology directed DNA repair

TREX1 is the most abundant 3’-5’ DNA exonuclease in mammals. Dominant C-terminal TREX1 genetic variants can disrupt homology-directed DNA repair and thus can trigger DNA damage and premature senescence phenotypes in humans, mice and ''Drosophila''. Such TREX1 variants can also cause adult onset small vessel disease known as vasculopathy with cerebral leukoencephalopathy.

References

Further reading

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External links

OMIM entries on Aicardi-Goutieres syndrome

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