Subtractive hybridization is a technology that allows for
PCR-based amplification of only
cDNA
In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA (miRNA)) template in a reaction catalyzed by the enzyme reverse transcriptase. cDNA is often used to express a speci ...
fragments that differ between a control (driver) and experimental
transcriptome. cDNA is produced from
mRNA. Differences in relative abundance of transcripts are highlighted, as are genetic differences between species. The technique relies on the removal of dsDNA formed by hybridization between a control and test sample, thus eliminating cDNAs or
gDNAs of similar abundance, and retaining differentially expressed, or variable in sequence, transcripts or genomic sequences.
Suppression subtractive hybridization has also been successfully used to identify strain- or species-specific DNA sequences in a variety of bacteria including ''Vibrio'' species (
Metagenomics).
See also
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Representational difference analysis
External links
Overview at evrogen.com*
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Biotechnology
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