Sf9 cells, a clonal isolate of '' Spodoptera frugiperda'' Sf21 cells (IPLB-Sf21-AE), are commonly used in

insect cell culture The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from ''Bombyx m ...

for recombinant protein production using baculovirus. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.

Sf9 Rhabdovirus

It has previously been shown that some Sf9 cell lines harbor a

negative sense In molecular biology and genetics, the sense of a nucleic acid molecule, particularly of a strand of DNA or RNA, refers to the nature of the roles of the strand and its complement in specifying a sequence of amino acids. Depending on the context, ...

Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV). However, not all tested Sf9 cells appear to be infected with this virus. SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.{{cite journal, author=Maghodia AB, Jarvis DL, title=Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines. , journal=Virology , year= 2017 , volume= 512 , issue= , pages= 234–245 , pmid=29024851 , doi=10.1016/j.virol.2017.09.025 , pmc=5651996

References

External links

Cellosaurus entry for Sf9
Insect cell lines Spodoptera