The selector technique is a method to

amplify Amplification or Amplified or Amplify may refer to: Science and technology * Amplification, the operation of an amplifier, a natural or artificial device intended to make a signal stronger * Amplification (molecular biology), a mechanism leading t ...

and

multiplex Multiplex may refer to: * Multiplex (automobile), a former American car make * Multiplex (comics), a DC comic book supervillain * Multiplex (company), a global contracting and development company * Multiplex (assay), a biological assay which meas ...

genomic DNA.

Process

Genomic DNA is digested with

restriction enzymes A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class o ...

, circularized by hybridisation to selectors and subsequently attached to a

vector Vector most often refers to: *Euclidean vector, a quantity with a magnitude and a direction *Vector (epidemiology), an agent that carries and transmits an infectious pathogen into another living organism Vector may also refer to: Mathematic ...

sequence by ligation. The procedure results in circular DNA

molecules A molecule is a group of two or more atoms held together by attractive forces known as chemical bonds; depending on context, the term may or may not include ions which satisfy this criterion. In quantum physics, organic chemistry, and bioc ...

with an included general primer pair motif that can be used for amplification by

PCR PCR or pcr may refer to: Science * Phosphocreatine, a phosphorylated creatine molecule * Principal component regression, a statistical technique Medicine * Polymerase chain reaction ** COVID-19 testing, often performed using the polymerase chain r ...

RCA The RCA Corporation was a major American electronics company, which was founded as the Radio Corporation of America in 1919. It was initially a patent pool, patent trust owned by General Electric (GE), Westinghouse Electric Corporation, Westin ...

Selector construct

A selector consists of two oligonucleotides, one Vector oligonucleotide and one Selector probe. Together they form one Selector with target specific ends on each side of a general primer motif.

Selection mechanisms

# A selector probe hybridizes with both ends of the selected target. # A selector probe hybridizes with one end to the 3’ end of the target and the other end to an internal sequence of the target. The protruding 5' end is cleaved off using

Taq polymerase ''Taq'' polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism ''Thermus aquaticus,'' from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to ''Taq'' or ''T ...

Publications

Demonstration of the selector methodThe PieceMaker software for designing selector experiments
{{DEFAULTSORT:Selector-Technique DNA