S27 may refer to:

Aviation

* Blériot-SPAD S.27, a French biplane airliner * Kalispell City Airport, in Flathead County, Montana, United States * Short S.27, a British military trainer * Sikorsky S-27, a Russian biplane bomber

Rail and transit

* S27 (Long Island bus), United States * S27 (Munich), a line of the Munich S-Bahn, Germany * S27 (Südostbahn), a railway service in Switzerland * S27, an

Aargau S-Bahn The Aargau S-Bahn ( or ) is an S-Bahn-style regional rail network serving the canton of Aargau, Switzerland, with some services extending into the cantons of Bern, Lucerne, Solothurn and Zug and one service to the German city of Waldshut-Tiengen ...

line in Switzerland and Germany * Rankoshi Station, in Hokkaido, Japan

Other uses

* 40S ribosomal protein S27 * British NVC community S27, a swamps and tall-herb fens community in the British National Vegetation Classification system * County Route S27 (California) * , a submarine of the Royal Navy * S27: Take off immediately all contaminated clothing, a safety phrase * Sulfur-27, an isotope of sulfur * , a submarine of the United States Navy {{Letter-NumberCombDisambig