RybB is a small
non-coding RNA
A non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein. The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene. Abundant and functionally important types of non- ...
was identified in a large scale screen of ''
Escherichia coli
''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Esc ...
''. The function of this short RNA has been studied using a transcriptomic approach and kinetic analyses of target mRNA decay ''in vivo''. RybB was identified as a factor that selectively accelerates the decay of multiple major omp mRNAs upon induction of the envelope stress response.
This RNA has been shown to bind to the
Hfq protein
The Hfq protein (also known as HF-I protein) encoded by the ''hfq'' gene was discovered in 1968 as an ''Escherichia coli'' host factor that was essential for replication of the bacteriophage Qβ. It is now clear that Hfq is an abundant bacterial R ...
.
In ''
Salmonella
''Salmonella'' is a genus of rod-shaped (bacillus) Gram-negative bacteria of the family Enterobacteriaceae. The two species of ''Salmonella'' are ''Salmonella enterica'' and '' Salmonella bongori''. ''S. enterica'' is the type species and is fur ...
'', direct interactions for RybB with the following targets have been verified experimentally: STM2391 (''fadL''), STM1070 (''ompA''), STM2267 (''ompC''), STM1572 (''ompD''), STM0999 (''ompF''), STM1473 (''ompN''), STM1995 (''ompS''), STM1732 (''ompW''), STM0413 (''tsx''), STM0687 (''ybfM'', ''chiP'') and STM1530.
In ''
Escherichia coli
''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Esc ...
'', direct interactions for RybB with the following targets have been verified experimentally: b0805 (''fiu''), b0721 (''sdhC''), b2215 (''ompC''), b1256 (''ompW''), b2594 (''rluD'') and b0081 (''mraZ'').
See also
*
RyfA RNA
The ryfA RNA gene is a non-coding RNA present in ''E. coli'', ''Shigella flexneri'' and ''Salmonella
''Salmonella'' is a genus of rod-shaped (bacillus) Gram-negative bacteria of the family Enterobacteriaceae. The two species of ''Salmonella' ...
*
RydB RNA
The RydB RNA is a non-coding RNA originally identified in ''E. coli'' in an RNA screen. This gene is only 67 nucleotides in length and is composed of a hairpin like structure. RydB lies between the ''ydiC'' and ''ydiH'' in ''E. coli''. Homolo ...
*
RydC RNA
RydC is a bacterial non-coding RNA. RydC is thought to regulate a mRNA, yejABEF, which encodes an ABC transporter protein. RydC is known to bind the Hfq protein, which causes a conformational change in the RNA molecule. The Hfq/RydC complex is th ...
*
MicA RNA
The MicA RNA (also known as SraD) is a small non-coding RNA that was discovered in ''E. coli'' during a large scale screen. Expression of SraD is highly abundant in Stationary phase (biology), stationary phase, but low levels could be detected in ...
References
External links
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Non-coding RNA
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