Route 16 may refer to:
* One of several highways - see
List of highways numbered 16
Route 16, or Highway 16, can refer to:
International
* Asian Highway 16
* European route E16
* European route E016
Australia
- Thompsons Road (Victoria)
- South Australia
Canada
;Parts of the Trans-Canada Highway:
*Yellowhead Hi ...
* One of several public transport routes - see
List of public transport routes numbered 16
*
Mystic Valley Parkway station, also called Route 16 - a proposed light rail station in Medford, Massachusetts
*
''Route-16'' (video game), a 1981 video game
{{Disambiguation