ROSA26 is a locus used for constitutive, ubiquitous gene expression in

mice A mouse ( : mice) is a small rodent. Characteristically, mice are known to have a pointed snout, small rounded ears, a body-length scaly tail, and a high breeding rate. The best known mouse species is the common house mouse (''Mus musculus' ...

. It was first isolated in 1991 in a gene-trap mutagenesis screen of

embryonic stem cells Embryonic stem cells (ESCs) are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage pre- implantation embryo. Human embryos reach the blastocyst stage 4–5 days post fertilization, at which time they cons ...

(ESCs). Over 130

knock-in In molecular cloning and biology, a gene knock-in (abbreviation: KI) refers to a genetic engineering method that involves the one-for-one substitution of DNA sequence information in a genetic locus or the insertion of sequence information not found ...

lines have been created based on the ROSA26 locus. The human

homolog In biology, homology is similarity due to shared ancestry between a pair of structures or genes in different taxa. A common example of homologous structures is the forelimbs of vertebrates, where the Bat wing development, wings of bats and Ori ...

of the ROSA26 locus has been identified. ROSA stands for Reverse Orientation Splice Acceptor, named after the

lentivirus ''Lentivirus'' is a genus of retroviruses that cause chronic and deadly diseases characterized by long incubation periods, in humans and other mammalian species. The genus includes the human immunodeficiency virus (HIV), which causes AIDS. Le ...

genetrap vector.

References

{{reflist Mouse genetics