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QuEChERS is a solid phase extraction method for detection of pesticide residues in food. The name is a
portmanteau word A portmanteau word, or portmanteau (, ) is a blend of words

Technique

The sample (
fruit In botany, a fruit is the seed-bearing structure in flowering plants that is formed from the ovary after flowering. Fruits are the means by which flowering plants (also known as angiosperms) disseminate their seeds. Edible fruits in partic ...
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vegetable Vegetables are parts of plants that are consumed by humans or other animals as food. The original meaning is still commonly used and is applied to plants collectively to refer to all edible plant matter, including the flowers, fruits, stems ...
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tobacco Tobacco is the common name of several plants in the genus '' Nicotiana'' of the family Solanaceae, and the general term for any product prepared from the cured leaves of these plants. More than 70 species of tobacco are known, but the ch ...
, etc.) is homogenized and centrifuged with a reagent and agitated for 1 minute. The reagents used depend on the type of sample to be analyzed. Following this, the sample is put through a dispersive solid phase extraction cleanup prior to analysis by gas-liquid chromatography or
liquid-liquid chromatography In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a ...
. Samples prepared using the QuEChERS method can be processed more quickly using a homogenization instrument. Such instruments can homogenize the food sample in a centrifuge tube, then agitate the sample with the reagent of choice, before moving the extracted sample for centrifuging. By using such an instrument, the samples can be moved through the QuEChERS method more quickly. Some modifications to the original QuEChERS method had to be introduced to ensure efficient extraction of pH-dependent compounds (e.g., phenoxyalkanoic acids), to minimize degradation of susceptible compounds (e.g., base and acid labile pesticides) and to expand the spectrum of matrices covered.


Applications

The QuEChERS method has been readily accepted by many pesticide residue analysts.A Novel Sample Preparation Approach to Increase the Throughput of Pesticide Analysis by LC–MS-MS


References

{{pesticides Food safety Separation processes