''Pseudomonas'' sRNA are

non-coding RNA A non-coding RNA (ncRNA) is a functional RNA molecule that is not Translation (genetics), translated into a protein. The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene. Abundant and functionally imp ...

s (ncRNA) that were predicted by the

bioinformatic Bioinformatics () is an interdisciplinary field of science Science is a systematic discipline that builds and organises knowledge in the form of testable hypotheses and predictions about the universe. Modern science is typically divi ...

program SRNApredict2. This program identifies putative sRNAs by searching for co-localization of genetic features commonly associated with sRNA-encoding genes and the

expression Expression may refer to: Linguistics * Expression (linguistics), a word, phrase, or sentence * Expression (mathematics), Symbolic description of a mathematical object * Fixed expression, a form of words with a specific meaning * Idiom, a type of ...

of the predicted sRNAs was subsequently confirmed by

Northern blot The northern blot, or RNA blot,Gilbert, S. F. (2000) Developmental Biology, 6th Ed. Sunderland MA, Sinauer Associates. is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.Ke ...

analysis. These sRNAs have been shown to be conserved across several ''

pseudomonas ''Pseudomonas'' is a genus of Gram-negative bacteria belonging to the family Pseudomonadaceae in the class Gammaproteobacteria. The 348 members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a ...

'' species but their function is yet to be determined. Using Tet-Trap genetic approach RNAT genes post-transcriptionally regulated by temperature upshift were identified: ''ptxS'' (implicated in virulence) and PA5194.

References

{{Navbox , name = hide the gallery , title = Gallery of Pseudomonas small RNA secondary structure images , titlestyle = background:#e7dcc3 , state = autocollapse , list1 = {{Gallery , Image:P2_secondary_structure.jpg, P2: Secondary structure taken from th
Rfam
database. Famil
RF01672
, Image:P10_secondary_structure.jpg, P10: Secondary structure taken from th
Rfam
database. Famil
RF01668
, Image:P13_secondary_structure.jpg, P13: Secondary structure taken from th
Rfam
database. Famil
RF01665
, Image:P14_secondary_structure.jpg, P14: Secondary structure taken from th
Rfam
database. Famil
RF01669
, Image:P17_secondary_structure.jpg, P17: Secondary structure taken from th
Rfam
database. Famil
RF01670
, Image:P18_secondary_structure.jpg, P18: Secondary structure taken from th
Rfam
database. Famil
RF01671
, Image:P20_secondary_structure.jpg, P20: Secondary structure taken from th
Rfam
database. Famil
RF01673
, Image:P27_secondary_structure.jpg, P27: Secondary structure taken from th
Rfam
database. Famil
RF01674
, Image:P29_secondary_structure.jpg, P29: Secondary structure taken from th
Rfam
database. Famil
RF01675
, Image:P31_secondary_structure.jpg, P31: Secondary structure taken from th
Rfam
database. Famil
RF01676
, Image:P35_secondary_structure.jpg, P35: Secondary structure taken from th
Rfam
database. Famil
RF01677
RNA

See also

References