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Profilin-1 is a
protein Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residue (biochemistry), residues. Proteins perform a vast array of functions within organisms, including Enzyme catalysis, catalysing metab ...
that in humans is encoded by the ''PFN1''
gene In biology, the word gene has two meanings. The Mendelian gene is a basic unit of heredity. The molecular gene is a sequence of nucleotides in DNA that is transcribed to produce a functional RNA. There are two types of molecular genes: protei ...
.


Structure

The PFN1 protein, also known as profilin-1, is a small, monomeric protein composed of 139–140 amino acids with a molecular mass of approximately 15 kDa. Its three-dimensional structure features an antiparallel, seven-stranded
β-sheet The beta sheet (β-sheet, also β-pleated sheet) is a common structural motif, motif of the regular protein secondary structure. Beta sheets consist of beta strands (β-strands) connected laterally by at least two or three backbone chain, backbon ...
core flanked by three
amphipathic In chemistry, an amphiphile (), or amphipath, is a chemical compound possessing both hydrophilic (''water-loving'', polar) and lipophilic (''fat-loving'', nonpolar) properties. Such a compound is called amphiphilic or amphipathic. Amphiphilic c ...
α-helices An alpha helix (or α-helix) is a sequence of amino acids in a protein that are twisted into a coil (a helix). The alpha helix is the most common structural arrangement in the secondary structure of proteins. It is also the most extreme type of l ...
—two on one side of the sheet and one on the other—forming a compact, globular fold known as the "profilin-like" fold. PFN1 contains three key functional domains: an
actin Actin is a family of globular multi-functional proteins that form microfilaments in the cytoskeleton, and the thin filaments in muscle fibrils. It is found in essentially all eukaryotic cells, where it may be present at a concentration of ...
-binding domain, a poly-L-proline (PLP)-binding domain, and a
phosphoinositide Phosphatidylinositol or inositol phospholipid is a biomolecule. It was initially called "inosite" when it was discovered by Léon Maquenne and Johann Joseph von Scherer in the late 19th century. It was discovered in bacteria but later also found ...
-binding domain. These domains enable PFN1 to interact with actin monomers, polyproline-rich motifs in various cytoskeletal proteins, and phosphoinositides, respectively, facilitating its central role in actin
cytoskeleton The cytoskeleton is a complex, dynamic network of interlinking protein filaments present in the cytoplasm of all cells, including those of bacteria and archaea. In eukaryotes, it extends from the cell nucleus to the cell membrane and is compos ...
dynamics. Structural studies, including NMR and X-ray crystallography, have shown that PFN1’s binding sites for actin and PLP are distinct and flexible, allowing the protein to adopt different conformations when free or bound to its ligands. This structural versatility is essential for PFN1’s function in actin polymerization and its involvement in numerous cellular processes.


Function

The protein encoded by this gene is a ubiquitously expressed actin monomer-binding protein belonging to the profilin family. It is believed to regulate actin polymerization in response to extracellular signals. Profilin-1 also functions as a pseudouridine-binding protein, contributing to the stability and translational efficiency of certain
mRNA In molecular biology, messenger ribonucleic acid (mRNA) is a single-stranded molecule of RNA that corresponds to the genetic sequence of a gene, and is read by a ribosome in the process of Protein biosynthesis, synthesizing a protein. mRNA is ...
s. Profilin 1 (PFN1) is a highly conserved actin-binding protein that plays a central role in regulating actin polymerization and, consequently, the dynamics of the actin cytoskeleton. By binding actin monomers, PFN1 promotes their incorporation into growing actin filaments, thereby influencing key cellular processes such as
cell motility Motility is the ability of an organism to move independently using metabolism, metabolic energy. This biological concept encompasses movement at various levels, from whole organisms to cells and subcellular components. Motility is observed in ...
,
membrane trafficking Membrane vesicle trafficking in eukaryotic animal cells involves movement of biochemical signal molecules from synthesis-and-packaging locations in the Golgi body to specific release locations on the inside of the plasma membrane of the secretory c ...
,
endocytosis Endocytosis is a cellular process in which Chemical substance, substances are brought into the cell. The material to be internalized is surrounded by an area of cell membrane, which then buds off inside the cell to form a Vesicle (biology and chem ...
,
cell cycle The cell cycle, or cell-division cycle, is the sequential series of events that take place in a cell (biology), cell that causes it to divide into two daughter cells. These events include the growth of the cell, duplication of its DNA (DNA re ...
progression, and cell survival. In addition to its canonical role in actin dynamics, PFN1 interacts with poly-L-proline–rich motifs found in various signaling and
cytoskeletal The cytoskeleton is a complex, dynamic network of interlinking protein filaments present in the cytoplasm of all Cell (biology), cells, including those of bacteria and archaea. In eukaryotes, it extends from the cell nucleus to the cell membrane ...
proteins, as well as with phosphoinositide lipids, thereby linking cytoskeletal regulation to intracellular signaling pathways. Recent studies have expanded the known functions of PFN1 to include roles in microtubule organization, mitochondrial homeostasis, and the regulation of autophagy and mitophagy. Disruption of PFN1 function through mutation or loss has been implicated in several diseases, including neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS), as well as various cancers and cardiovascular conditions. PFN1 thus acts as a key integrator of cytoskeletal dynamics and intracellular signaling, playing a critical role in maintaining normal cell function and viability.


Clinical significance

Deletion of this gene is associated with Miller-Dieker syndrome. Mutations in this gene may be a rare cause of
amyotrophic lateral sclerosis Amyotrophic lateral sclerosis (ALS), also known as motor neuron disease (MND) or—in the United States—Lou Gehrig's disease (LGD), is a rare, Terminal illness, terminal neurodegenerative disease, neurodegenerative disorder that results i ...
, also called Lou Gehrig's disease.


Interactions

Profilin 1 has been shown to interact with: * FMNL1, *
MLLT4 Afadin is a protein that in humans is encoded by the ''AFDN'' gene. Function Afadin is a Ras (see HRAS; MIM 190020) target that regulates cell–cell adhesions downstream of Ras activation. It is fused with MLL (MIM 159555) in leukemias caused ...
*
Vasodilator-stimulated phosphoprotein Vasodilator-stimulated phosphoprotein is a protein that in humans is encoded by the ''VASP'' gene. Function Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an N-terminal ...
, * WASF1, and * WASL.


References


Further reading

* * * * * * * * * * * * * * * * * * {{Cytoskeletal Proteins