An outron is a

nucleotide sequence A nucleic acid sequence is a succession of bases within the nucleotides forming alleles within a DNA (using GACT) or RNA (GACU) molecule. This succession is denoted by a series of a set of five different letters that indicate the order of the nu ...

at the 5' end of the

primary transcript A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNA ...

of a

gene In biology, the word gene has two meanings. The Mendelian gene is a basic unit of heredity. The molecular gene is a sequence of nucleotides in DNA that is transcribed to produce a functional RNA. There are two types of molecular genes: protei ...

that is removed by a special form of

RNA splicing RNA splicing is a process in molecular biology where a newly-made precursor messenger RNA (pre-mRNA) transcription (biology), transcript is transformed into a mature messenger RNA (Messenger RNA, mRNA). It works by removing all the introns (non-cod ...

during maturation of the final RNA product. Whereas

intron An intron is any nucleotide sequence within a gene that is not expressed or operative in the final RNA product. The word ''intron'' is derived from the term ''intragenic region'', i.e., a region inside a gene."The notion of the cistron .e., gen ...

sequences are located inside the gene, outron sequences lie outside the gene.

Characteristics

The outron is an intron-like sequence possessing similar characteristics such as the G+C content and a splice acceptor site that is the signal for ''trans''-splicing. Such a ''trans''-splice site is essentially defined as an acceptor (3') splice site without an upstream donor (5') splice site. In eukaryotes such as euglenozoans,

dinoflagellates The Dinoflagellates (), also called Dinophytes, are a monophyletic group of single-celled eukaryotes constituting the phylum Dinoflagellata and are usually considered protists. Dinoflagellates are mostly marine plankton, but they are also commo ...

sponges Sponges or sea sponges are primarily marine invertebrates of the animal phylum Porifera (; meaning 'pore bearer'), a basal clade and a sister taxon of the diploblasts. They are sessile filter feeders that are bound to the seabed, and ar ...

nematodes The nematodes ( or ; ; ), roundworms or eelworms constitute the phylum Nematoda. Species in the phylum inhabit a broad range of environments. Most species are free-living, feeding on microorganisms, but many are parasitic. Parasitic worms (he ...

cnidarians Cnidaria ( ) is a phylum under kingdom Animalia containing over 11,000 species of aquatic invertebrates found both in fresh water, freshwater and marine environments (predominantly the latter), including jellyfish, hydroid (zoology), hydroids, ...

ctenophores Ctenophora (; : ctenophore ) is a phylum of marine invertebrates, commonly known as comb jellies, that marine habitats, inhabit sea waters worldwide. They are notable for the groups of cilia they use for swimming (commonly referred to as "combs ...

flatworms Platyhelminthes (from the Greek πλατύ, ''platy'', meaning "flat" and ἕλμινς (root: ἑλμινθ-), ''helminth-'', meaning "worm") is a phylum of relatively simple bilaterian, unsegmented, soft-bodied invertebrates commonly called ...

crustaceans Crustaceans (from Latin meaning: "those with shells" or "crusted ones") are invertebrate animals that constitute one group of Arthropod, arthropods that are traditionally a part of the subphylum Crustacea (), a large, diverse group of mainly aquat ...

, chaetognaths,

rotifers The rotifers (, from Latin 'wheel' and 'bearing'), sometimes called wheel animals or wheel animalcules, make up a phylum (Rotifera ) of microscopic and near-microscopic pseudocoelomate animals. They were first described by Rev. John Harris ...

, and tunicates, the length of spliced leader (SL) outrons range from 30 to 102 nucleotides (nt), with the SL exon length ranging from 16 to 51 nt, and the full SL RNA length ranging from 46 to 141 nt.

Processing

In standard cis-splicing, the donor splice site in upstream position is required together with an acceptor site located on downstream position on the same pre-RNA molecule. By contrast, the SL ''trans''-splicing relies on a 3' acceptor splice site on the outron, and a 5' donor splice site (GU dinucleotide) located on a separate RNA molecule, the SL RNA. Moreover, the outron of the premature mRNA contains a branchpoint

adenosine Adenosine (symbol A) is an organic compound that occurs widely in nature in the form of diverse derivatives. The molecule consists of an adenine attached to a ribose via a β-N9- glycosidic bond. Adenosine is one of the four nucleoside build ...

— followed by a downstream

polypyrimidine tract {{Short description, A pyrimidine-rich sequence involved in pre-messenger RNA maturation The polypyrimidine tract is a region of pre-messenger RNA (mRNA) that promotes the assembly of the spliceosome, the protein complex specialized for carrying ...

— which interacts with the intron-like portion of the SL RNA to form a 'Y' branched byproduct, reminiscent of the

lasso A lasso or lazo ( or ), also called reata or la reata in Mexico, and in the United States riata or lariat (from Mexican Spanish lasso for roping cattle), is a loop of rope designed as a restraint to be thrown around a target and tightened when ...

structure formed during intron splicing. Nuclear machinery then resolves this 'Y' branching structure by ''trans''-splicing the SL RNA sequence to the 3′ ''trans''-splice acceptor site (AG dinucleotide) of the pre-mRNA. When outrons are processed, the SL exon is ''trans''-spliced to distinct, unpaired, downstream acceptor sites adjacent to each

open reading frame In molecular biology, reading frames are defined as spans of DNA sequence between the start and stop codons. Usually, this is considered within a studied region of a prokaryotic DNA sequence, where only one of the six possible reading frames ...

of the polycistronic pre-mRNA, leading to distinct mature capped transcripts.

References

{{reflist Spliceosome RNA splicing Gene expression Non-coding DNA

Characteristics

Processing

See also

References