In
chemistry
Chemistry is the scientific study of the properties and behavior of matter. It is a physical science within the natural sciences that studies the chemical elements that make up matter and chemical compound, compounds made of atoms, molecules a ...
, orthochromasia is the property of a
dye or stain to not change color on binding to a target, as opposed to
''metachromatic'' stains, which do change color. The word is derived from the Greek ''
orthos'' (correct, upright), and chromatic (color).
Toluidine blue is an example of a partially orthochromatic dye, as it stains
nucleic acids
Nucleic acids are large biomolecules that are crucial in all cells and viruses. They are composed of nucleotides, which are the monomer components: a 5-carbon sugar, a phosphate group and a nitrogenous base. The two main classes of nucleic a ...
by its orthochromatic color (blue), but stains mast cell granules in its metachromatic color (red).
In
spectral terms, orthochromasia refers to maintaining the position of spectral peaks, while metachromasia refers to a shift in wavelength, becoming either
shorter or
longer.
In photography, an orthochromatic light spectrum is one devoid of red light.
Orthochromatic photography
Orthochromatic photography refers to a
photographic emulsion that is sensitive to blue and green light but not red light. This type of emulsion was a significant advancement in early photography, as it allowed for the production of images with more accurate tonal reproduction than the earlier emulsions that were sensitive only to blue (and ultraviolet) light,
e.g., the wet plate
collodion emulsions.
The development of orthochromatic films can be traced back to the work of
Hermann Wilhelm Vogel in 1873.
[ Vogel experimented with adding small amounts of certain aniline-based dyes to photographic emulsions to extend their sensitivity beyond blue light. This breakthrough allowed for the production of emulsions that could capture a broader spectrum of colors and tones including Josef Maria Eder, who introduced the use of the red dye erythrosine in 1884.]
In addition to their use in still photography, orthochromatic films also played a significant role in the early days of motion pictures. The improved tonal range provided by orthochromatic emulsions allowed filmmakers to create more visually compelling and realistic moving images.
Because orthochromatic emulsions are not sensitive to red light, they can be processed with a red safelight.
Relative to panchromasia
Most modern black-and-white films are panchromatic, which add sensitivity to red light, unlike orthochromatic emulsions, which are sensitive to only blue and green. Panchromatic films have a reduced sensitivity to blue light, compared to orthochromatic films.
The increased blue sensitivity of orthochromatic photography causes blue objects to appear lighter, and red ones darker than panchromatic emulsions. A cyan
Cyan () is the color between blue and green on the visible spectrum of light. It is evoked by light with a predominant wavelength between 500 and 520 nm, between the wavelengths of green and blue.
In the subtractive color system, or CMYK c ...
photographic filter, which blocks red light but allows blue and green to pass, can be used with standard panchromatic film to produce a similar effect. This technique allowed photographers to manipulate the tonal range of their images without relying on specialized orthochromatic films.
Despite the advancements in photographic technology that have occurred since the introduction of orthochromatic films, they continue to be appreciated by some photographers for their unique tonal qualities and artistic potential.
See also
* Panchromatic
References
Photographic film types
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