O31 may refer to:
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Douglas O-31, an observation aircraft of the United States Army Air Corps
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Healdsburg Municipal Airport, in Sonoma County, California, United States
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Otoyol 31
Otoyol 31, named the İzmir-Denizli Motorway () and abbreviated as the O-31 is a long toll motorway in western Turkey. Beginning at an intersection with İzmir Beltway, the O-31 runs south from İzmir to Denizli and parallels the D550 and D320 ...
, a motorway in Turkey
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