Nephelometry
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A nephelometer or aerosol photometer is an instrument for measuring the concentration of suspended particulates in a
liquid Liquid is a state of matter with a definite volume but no fixed shape. Liquids adapt to the shape of their container and are nearly incompressible, maintaining their volume even under pressure. The density of a liquid is usually close to th ...
or
gas Gas is a state of matter that has neither a fixed volume nor a fixed shape and is a compressible fluid. A ''pure gas'' is made up of individual atoms (e.g. a noble gas like neon) or molecules of either a single type of atom ( elements such as ...
colloid A colloid is a mixture in which one substance consisting of microscopically dispersed insoluble particles is suspended throughout another substance. Some definitions specify that the particles must be dispersed in a liquid, while others exte ...
. A nephelometer measures suspended particulates by employing a light beam (source beam) and a light detector set to one side (often 90°) of the source beam. Particle
density Density (volumetric mass density or specific mass) is the ratio of a substance's mass to its volume. The symbol most often used for density is ''ρ'' (the lower case Greek letter rho), although the Latin letter ''D'' (or ''d'') can also be u ...
is then a function of the light
reflected Reflection is the change in direction of a wavefront at an interface between two different media so that the wavefront returns into the medium from which it originated. Common examples include the reflection of light, sound and water waves. The ...
into the detector from the particles. To some extent, how much light reflects for a given density of particles is dependent upon properties of the particles such as their shape,
color Color (or colour in English in the Commonwealth of Nations, Commonwealth English; American and British English spelling differences#-our, -or, see spelling differences) is the visual perception based on the electromagnetic spectrum. Though co ...
, and
reflectivity The reflectance of the surface of a material is its effectiveness in Reflection (physics), reflecting radiant energy. It is the fraction of incident electromagnetic power that is reflected at the boundary. Reflectance is a component of the respon ...
. Nephelometers are calibrated to a known particulate, then use environmental factors (k-factors) to compensate lighter or darker colored dusts accordingly. K-factor is determined by the user by running the nephelometer next to an air sampling pump and comparing results. There are a wide variety of research-grade nephelometers on the market as well as
open source Open source is source code that is made freely available for possible modification and redistribution. Products include permission to use and view the source code, design documents, or content of the product. The open source model is a decentrali ...
varieties.


Nephelometer uses

The main uses of nephelometers relate to air quality measurement for pollution monitoring, climate monitoring, and visibility. Airborne particles are commonly either biological contaminants, particulate contaminants, gaseous contaminants, or dust. The accompanying chart shows the types and sizes of various particulate contaminants. This information helps understand the character of particulate pollution inside a building or in the ambient air, as well as the cleanliness level in a controlled environment. Biological contaminants include mold, fungus, bacteria, viruses, animal dander, dust mites, pollen, human skin cells, cockroach parts, or anything alive or living at one time. They are the biggest enemy of
indoor air quality Indoor air quality (IAQ) is the air quality within buildings and Nonbuilding structure, structures. Poor indoor air quality due to indoor air pollution is known to affect the health, comfort, and well-being of building occupants. It has also be ...
specialists because they are contaminants that cause health problems. Levels of biological contamination depend on humidity and temperature that supports the livelihood of micro-organisms. The presence of pets, plants, rodents, and insects will raise the level of biological contamination.


Sheath air

Sheath air is clean filtered air that surrounds the aerosol stream to prevent particulates from circulating or depositing within the optic chamber. Sheath air prevents contamination caused by build-up and deposits, improves response time by containing the sample, and improves maintenance by keeping the optic chamber clean. The nephelometer creates the sheath air by passing air through a zero filter before beginning the sample.


Global radiation balance

Nephelometers are also used in global warming studies, specifically measuring the global radiation balance. Three wavelength nephelometers fitted with a backscatter shutter can determine the amount of solar radiation that is reflected back into space by dust and particulate matter. This reflected light influences the amount of radiation reaching the earth's lower atmosphere and warming the planet.


Visibility

Nephelometers are also used for measurement of visibility with simple one-wavelength nephelometers used throughout the world by many EPAs. Nephelometers, through the measurement of light scattering, can determine visibility in distance through the application of a conversion factor called Koschmieder's formula.


Medicine

In medicine, nephelometry is used to measure immune function. It is also used in clinical microbiology, for preparation of a standardized inoculum (McFarland suspension) for antimicrobial susceptibility testing.


Fire detection

Gas-phase nephelometers are also used in the detection of
smoke Smoke is an aerosol (a suspension of airborne particulates and gases) emitted when a material undergoes combustion or pyrolysis, together with the quantity of air that is entrained or otherwise mixed into the mass. It is commonly an unwante ...
and other particles of
combustion Combustion, or burning, is a high-temperature exothermic redox chemical reaction between a fuel (the reductant) and an oxidant, usually atmospheric oxygen, that produces oxidized, often gaseous products, in a mixture termed as smoke. Combustion ...
. In such use, the apparatus is referred to as an aspirated smoke detector. These have the capability to detect extremely low particle concentrations (to 0.005%) and are therefore highly suitable to protecting sensitive or valuable electronic equipment, such as
mainframe computer A mainframe computer, informally called a mainframe or big iron, is a computer used primarily by large organizations for critical applications like bulk data processing for tasks such as censuses, industry and consumer statistics, enterprise ...
s and
telephone switch A telephone exchange, telephone switch, or central office is a central component of a telecommunications system in the public switched telephone network (PSTN) or in large enterprises. It facilitates the establishment of communication circuits ...
es.


Turbidity units

*Because optical properties depend on suspended particle size, a stable synthetic material called "
Formazin Formazine (formazin) is a heterocyclic polymer produced by reaction of hexamethylenetetramine with hydrazine sulfate. The hexamethylenetetramine tetrahedral cage-like structure, similar to adamantane, serves as molecular building block to form ...
" with uniform particle size is often used as a standard for calibration and reproducibility. The unit is called Formazin Turbidity Unit (FTU). *Nephelometric Turbidity Units (NTU) specified by
United States Environmental Protection Agency The Environmental Protection Agency (EPA) is an independent agency of the United States government tasked with environmental protection matters. President Richard Nixon proposed the establishment of EPA on July 9, 1970; it began operation on De ...
is a special case of FTU, where a white light source and certain geometrical properties of the measurement apparatus are specified. (Sometimes the alternate form "nephelos turbidity units" is used''Florida Department of Agriculture and Consumer Services 2006 Codebook Chapter 5L-1: The Comprehensive Shellfish Control code''
) *Formazin Nephelometric Units (FNU), prescribed for 9 measurements of turbidity in water treatment by
ISO 7027 ISO 7027:1999 is an ISO standard for water quality that enables the determination of turbidity. The ISO 7027 technique is used to determine the concentration of suspended particles in a sample of water by measuring the incident light scattered at ...
, another special case of FTU with near infrared light (NIR) and 90° scatter. *Formazin Attenuation Units (FAU) specified by
ISO 7027 ISO 7027:1999 is an ISO standard for water quality that enables the determination of turbidity. The ISO 7027 technique is used to determine the concentration of suspended particles in a sample of water by measuring the incident light scattered at ...
for water treatment standards for turbidity measurements at 0°, also a special case of FTU. *Formazin Backscatter Units (FBU), not part of a standard, is the unit of optical backscatter detectors (OBS), measured at c. 180°, also a special case of FTU. *European Brewery Convention (EBC) turbidity units *Concentration Units (C.U.) *Optical Density (O.D.) *Jackson "Candle" Turbidity Units (JTU; an early measure) *Helms Units *American Society of Brewing Chemists (ASBC-FTU) turbidity units *Brantner Haze Scale (BHS) and Brantner Haze Units (BHU) for purposefully hazy beer *Parts Per Million of standard substance, such as PPM/DE (Kieselguhr) *"Trübungseinheit/Formazin" (TE/F) a German standard, now replaced by the FNU unit. *diatomaceous earth ("ppm SiO2") an older standard, now obsolete A more popular term for this instrument in water quality testing is a turbidimeter. However, there can be differences between models of turbidimeters, depending upon the arrangement (
geometry Geometry (; ) is a branch of mathematics concerned with properties of space such as the distance, shape, size, and relative position of figures. Geometry is, along with arithmetic, one of the oldest branches of mathematics. A mathematician w ...
) of the source beam and the detector. A nephelometric turbidimeter always monitors light reflected off the particles and not
attenuation In physics, attenuation (in some contexts, extinction) is the gradual loss of flux intensity through a Transmission medium, medium. For instance, dark glasses attenuate sunlight, lead attenuates X-rays, and water and air attenuate both light and ...
due to cloudiness. In the
United States The United States of America (USA), also known as the United States (U.S.) or America, is a country primarily located in North America. It is a federal republic of 50 U.S. state, states and a federal capital district, Washington, D.C. The 48 ...
environmental monitoring Environmental monitoring is the processes and activities that are done to characterize and describe the state of the environment. It is used in the preparation of environmental impact assessments, and in many circumstances in which human activit ...
the
turbidity Turbidity is the cloudiness or haziness of a fluid caused by large numbers of individual particles that are generally invisible to the naked eye, similar to smoke in air. The measurement of turbidity is a key test of both water clarity and wa ...
standard unit is called Nephelometric Turbidity Units (NTU), while the international standard unit is called Formazin Nephelometric Unit (FNU). The most generally applicable unit is Formazin Turbidity Unit (FTU), although different measurement methods can give quite different values as reported in FTU (see below). Gas-phase nephelometers are also used to study the
atmosphere An atmosphere () is a layer of gases that envelop an astronomical object, held in place by the gravity of the object. A planet retains an atmosphere when the gravity is great and the temperature of the atmosphere is low. A stellar atmosph ...
. These can provide information on
visibility In meteorology, visibility is the measure of the distance at which an object or light can be clearly discerned. It depends on the Transparency and translucency, transparency of the surrounding air and as such, it is unchanging no matter the amb ...
and atmospheric
albedo Albedo ( ; ) is the fraction of sunlight that is Diffuse reflection, diffusely reflected by a body. It is measured on a scale from 0 (corresponding to a black body that absorbs all incident radiation) to 1 (corresponding to a body that reflects ...
.


See also

*
ISO 7027 ISO 7027:1999 is an ISO standard for water quality that enables the determination of turbidity. The ISO 7027 technique is used to determine the concentration of suspended particles in a sample of water by measuring the incident light scattered at ...
*
Water purification Water purification is the process of removing undesirable chemicals, biological contaminants, suspended solids, and gases from water. The goal is to produce water that is fit for specific purposes. Most water is purified and disinfected for hu ...


References

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