Neoschizomers are
restriction enzymes
A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class o ...
that recognize the same
nucleotide sequence
A nucleic acid sequence is a succession of bases within the nucleotides forming alleles within a DNA (using GACT) or RNA (GACU) molecule. This succession is denoted by a series of a set of five different letters that indicate the order of the nu ...
, but cleave at different sites. The first restriction enzyme discovered to recognize a sequence is called the prototype, and others that recognize the same sequence are
isoschizomers. Neoschizomers are a subset of isoschizomers.
For example, MaeII is the prototype enzyme for the sequence "ACGT", with the cleavage site A↓CGT. One of its neoschizomers, Tsp49I, also recognizes the sequence "ACGT", but cleaves at ACGT↓.
Another example is SmaI (CCC↓GGG), which is a neoschizomer of XmaI (C↓CCGGG).
Use in molecular biology
External links
* Rebase restriction enzyme database, http://rebase.neb.com/rebase/
See also
*
Isoschizomer
*
Isocaudomer
*
List of restriction enzyme cutting sites
A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria. One special kind of restriction enzymes is the class of " homing endonucleases", these bein ...
References
Restriction enzymes
{{enzyme-stub
pl:Izoschizomery