NSP5 (
nonstructural protein 5) encoded by genome segment 11 of group A
rotavirus
Rotaviruses are the most common cause of diarrhea, diarrhoeal disease among infants and young children. Nearly every child in the world is infected with a rotavirus at least once by the age of five. Immunity (medical), Immunity develops with ...
es. In virus-infected cells NSP5 accumulates in the
viroplasm
A viroplasm, sometimes called "virus factory" or "virus inclusion", is an Inclusion bodies, inclusion body in a Cell (biology), cell where viral replication and assembly occurs. They may be thought of as viral factories in the cell. There are many ...
s. NSP5 has been shown to be autophosphorylated.
Interaction of NSP5 with
NSP2 was also demonstrated.
In
rotavirus
Rotaviruses are the most common cause of diarrhea, diarrhoeal disease among infants and young children. Nearly every child in the world is infected with a rotavirus at least once by the age of five. Immunity (medical), Immunity develops with ...
-infected cells, the non-structural proteins NSP5 and
NSP2 localize in complexes called
viroplasm
A viroplasm, sometimes called "virus factory" or "virus inclusion", is an Inclusion bodies, inclusion body in a Cell (biology), cell where viral replication and assembly occurs. They may be thought of as viral factories in the cell. There are many ...
s, where replication and assembly occur and they can drive the formation of viroplasm-like structures in the absence of other rotaviral proteins and rotavirus replication.
There is no atomic-resolution structure of NSP5 determined as of June 2019. However, the low resolution three-dimensional structure of the NSP2-NSP5 assembly has been observed by
cryo-EM. NSP5 occupies the same site as RNA when binding to NSP2. The EM data from this 2006 study has not been published.
References
Rotaviruses
Viral nonstructural proteins
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